Molecular Diagnosis of Mycoplasma pneumoniae in Synovial Fluid of Rheumatoid Arthritis Patients (original) (raw)
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مجله دانش و تندرستي, 2013
Introduction: Cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) is an important negative regulator of Tcell responses. The 49AG polymorphism of the CTLA-4 gene may be associated with systemic lupus erythematous (SLE) risk, but the results from previous published studies have been inconsistent. We carried out a meta-analysis search to assess this association more precisely. Methods: A systematic search of six electronic databases (PubMed, Science Direct, OVID, Iran doc, Iran Medex and SID (Scientific Information Database) was performed for relevant articles published between 1978 and 2011. The odds ratio (OR) and its 95% confidence interval (95%CI) were used to assess the strength of the association. We evaluated both fixed and random effect models, depending on the presence of between-study heterogeneity. The data were analyzed using STATA software. Results: A total of 15 independent studies on the CTLA-4 gene 49AG polymorphism and SLE, including 1705 cases and 2299 controls were used in the meta-analysis. No significant association was found between 49AG polymorphism and SLE risk in the overall or subgroup analyses. Conclusion: This meta-analysis showed no significant association between 49AG polymorphism and SLE susceptibility. Large-scale and well-designed case-control studies are suggested.
New Cellular and Molecular Biotechnology Journal, 2019
Aim and Background: Klebsiella pneumoniae is a common cause of β-lactamase-associated infections in hospitals. The present study aimed to determine the frequency of antibiotic resistance genes in Klebsiella pneumoniae strains producing IMP-1 and TEM β-lactamase. Materials and methods: The present research identified 94 samples of K. pneumoniae, using antibiogram for the phenotypic confirmation of ESBLs. The antibiotic resistance of the isolates and the prevalence of TEM and IMP-1 genes were determined using PCR method. Findings: Of 94 samples, 77.6% were ESBL-positive and 22.3% ESBL-negative. A total of 4.1% of the samples carried the IMP-1 gene and 43.8% the TEM gene, while 43.8% of the samples carried both genes. Conclusion: Given that TEM and IMP-1 genes were commonly present in a large number of the resistant samples, physicians are recommended to use therapeutic measures properly, and to prescribe antibiotics rationally.
Journal of Mazandaran University of Medical Sciences, 2016
Background and purpose: Platelets communicate with different immune cells and can activate B-lymphocytes and induce the production of antibodies from these cells. Platelet microparticles (MPs) originate from platelets and express the surface markers of platelets. This study aimed at investigating the ability of these microvesicles on production of antibodies from B-lymphocytes. Materials and methods: In this experimental study, platelet MPs were isolated from platelet concentrates and B cells were isolated from human whole blood. Then MPs were co-cultured with Blymphocytes. In different days of culture, the production of IgG antibodies was studied in the supernatants of culture medium using ELISA method. The results were analyzed by paired-samples t-test. Pvalue < 0.05 was considered statistically significant. Results: Platelet MPs stimulate the production of antibodies by B-lymphocytes. During 5-day coculture, significant increase was observed in the production of IgG antibodies...
Qom University of Medical Sciences Journal, 2017
Accepted: 29 Mar, 2016 Abstract Background and Objectives: AmpC beta-lactamases are among cephalosporinases encoded on the chromosomes of many Enterobacteriaceae. In many bacteria, induction of AmpC enzymes can be made at a very high level by numerous mutations. In this study, the prevalence of chromosomal AmpC genes, was investigated in the isolates of Pseudomonas aeruginosa isolated from teaching hospitals in Zahedan city in 2015.
Acinetobacter baumannii: Researchers' Scientific Cooperation Network in Three Decades
Iranian Journal of Medical Microbiology, 2020
Background: Acinetobacter baumannii is one of the most common challenging pathogens in causing serious infections in intensive care units of modern hospital systems around the world and poses a serious threat to public and patient health. This study aims to analyze the network of scientific and empirical collaborations of A. baumannii researchers in the last three decades. Materials & Methods: The present study was performed using the Co-citation analysis technique. All A. baumannii publications indexed in the Web of Science Core Collection for the period 1990-2019 are the statistical population of the study. After an advanced search, 4473 documents were retrieved. A total of 18343 authors contributed to the publication of the retrieved documents. Ravar PreMap 1.0.0.0, NetDraw, and UCINET 6.528.0.0 software were utilized for data analysis. Results: Data analysis showed that the global publication of A. baumannii has risen. "Clinical Infectious Diseases," was the best journal, and "Seifert, Harald," the most influential researcher, and "Seifert, Harald * Higgins, Paul G," were identified as the best co-citation pair. Top researchers in A. baumannii were "Beceiro," "Alejandro," "HSU Li Yang," and "Seifert, Harald," respectively, based on degree, betweenness and closeness centrality indicators. Conclusion: Analysis of social networks A. baumannii presents an objective and realistic view to experts and planners in Medical Sciences. Also, the structure of A. baumannii's internal relationships and researchers' connections is determined objectively. Finally, researchers get acquainted with journals, scientists and organizations that are proliferated and effective and plan to collaborate with them in the future
The first report of pneumocystis carini pneumonia in a patient with HIV infection in Kermanshah
Journal of Kermanshah University of Medical Sciences, 2003
Background and Objective : Pneumocystis carini pneumonia is one of the most important opportunistic infections in patients with HIV/AIDS. Occurrence of this disease reflects marked immunodeficiency and a CD4 count of less than 200 per microliter of serum. Case report: A 25 years old HIV positive man was referred to the Sina hospital with aspiration pneumonia following a suicide. Because of severe dyspnea, cyanosis, continuous fever and cough, and lack of response to ordinary treatments, pneumocystis carini pneumonia was suspected and proved by sputum examination. The patient's condition improved after 2 weeks of therapy, but soon after discharge, clinical deterioration occurred and the patient died within 24 hours. Discussion: The patient's death may be the result of lack of therapeutic compliance by the patient, or concomitant occurrence of other opportunistic infections. Occurrence of such diseases as pneumocystis carini pneumonia may sugges begining of a new stage in ...
Iranian Journal of Medical Microbiology, 2019
Article Subject: Molecular Microbiology DOI: Background and Aims: High prevalence of Methicillin Resistant Staphylococcus Aureus isolates (MRSA) as well as the multi-drug resistance in this bacterium causes difficulties in the treatment of infections due to these bacteria. Hence, detection of MRSA isolates by rapid and accurate methods is necessary. PCR-ELISA is an accurate and molecular technique that is used for the detection of several pathogens. The aim of this study is the detection of MRSA using PCR-ELISA. Materials and Methods: Specific primers for mecA gene were designed. Then, dNTP labeled with Digoxigenin was applied for amplifying mecA gene. DIG-labeled PCR products were seeded on the well coated streptoavidin and identified by anti-DIG-peroxidase conjugate. Furthermore, Biotin-labeled DNA probe specific for mecA gene was used. Sensitivity and specificity of the method was determined. Resistance to methicillin among 70 clinical isolates was determined by the disk diffusion, agar dilution and PCR-ELISA methods. Results: MecA gene of S. aureus was amplified using gene specific primers resulted in a fragment with 310 bp length. Findings from the PCR-ELISA technic showed no cross-reactivity with Klebsiella Pneumoniae, Bacillus subtilis and Esheriashia coli as control bacteria and its sensitivity was 0.5 ng. The prevalence of MRSA clinical isolates by the disk diffusion, agar dilution and PCR-ELISA methods was 60%, 58.5% and 60%, respectively. Conclusion:The PCR-ELISA technique was known as an accurate and rapid test for the detection of infection agents using their specific gene. This technic can applied as an appropriate alternative method for time-consuming, less sensitive and expensive techniques such as Real-time PCR and differential biochemical tests which are currently used in laboratory.
Journal of Mazandaran University of Medical Sciences, 2018
Background and purpose: Increasing resistance to Quinolones in Escherichia coli and Klebsiella pneumonia in Sari, has caused many problems in treatment. Mutation in gyrA gene lead to changes in amino acids and resistance against Fluoroquinolones in E. coli and K. pneumonia. This study aimed at identifying remarkable mutations in E. coli and K. pneumonia isolates using PCR-SSCP analysis. Materials and methods: Antibiotic sensitivity test (ciprofloxacin, nalidixic acid) was performed using Agar Disk Diffusion method. Resistance to fluoroquinolones was confirmed by E-test. (MIC experiment). We used PCR-SSCP method to detect mutation in gyrA (ser83 – asp 87) genes. Then, the PCR products were randomly sequenced. Results: From 103 isolates, 65 (63.2 %) were E. coli and 38 (36.8%) were K. pneumoniae. In all E.coli isolates resistant to Ciprofloxacin, at least one mutation was observed. Also, in all K. pneumoniae samples resistant to Ciprofloxacin, at least one mutation was seen and in 14 ...
Iranian Journal of Medical Microbiology
Background and Aims: Oncolytic viruses (OVs) are a new approach in treatment of cancer. Antitumor efficacy of OVs were limited due to insufficient and non-specific viral delivery to tumor sites. To overcome this issue, mesenchymal stem cells (MSCs) were used for their ability to specifically homing into tumors. The main aim of this study was to use MSCs as carriers and investigate the effect of oncolytic reovirus infection in MSCs, induction of apoptosis, nitric oxide (NO) secretion and their effects for selectively killing tumor cells, to use in future. Materials and Methods: MSCs isolated from mice adipose tissue and confirmed. Then, the ability of the virus to infect MSCs and the effect of reovirus infection in induction of apoptosis and NO secretion in MSCs were evaluated. Results: The results demonstrate that reovirus could replicate on MSCs. The finding indicated that the NO production significantly was higher at 72 h post infection with different MOI in comparison to the control cells. Also, reovirus induced high level of apoptosis in the MSCs at 48 h post infection compared with the control cells. Conclusions: Based on observed results, reovirus increased the secretion of iNOS (inducible nitric oxide) in the infected MSCs at 48 h post infection; therefore, high amounts of NO and reovirus replication were found to trigger apoptosis at 48 h post infection. Therefore, by optimizing the replication time of virus in the MSCs, specific viral delivery to tumor sites are available and causes cancer cells' death.