Effects of the probiotic, Lactobacillus acidophilus , on the growth performance, haematology parameters and immunoglobulin concentration in African Catfish ( Clarias gariepinus , Burchell 1822) fingerling (original) (raw)
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Action of Dietary Lactobacillus in catfish - Akinwande and Awodiran
The efficacy of dietary Lactobacillus acidophilus as an immunostimulant against Aeromonas hydrophilia in Heterobranchus longifilis broodstocks was investigated. Forty-eight H. longifilis broodstocks (720 ± 35 g) were randomly distributed in twelve concrete tanks (4 m 3 ) with continuous flow-through system at a stocking density of 1 fish/m 3 . Two groups of fish were fed a diet not supplemented with L. acidophilus (the non-probiotics diet), while the other two groups were fed a diet supplemented with L. acidophilus, comprising about 4.22 x10 4 colony-forming units per gram of diet (the probiotics diet). In treatment 1, H. longifilis broodstocks were injected with 0.5 mL physiological saline and fed the non-probiotic diet (non-probiotic control; T1). In treatment 2, broodstocks were injected with 0.5mL of A. hydrophila, respectively and were all fed the non-probiotic diet (designated as non-probiotic treatment; T2). In treatment 3, broodstocks were injected with 0.5 mL physiological saline but fed the probiotic diet (probiotic control; T3), while broodstocks in treatment 4 were injected with 0.5 mL, of A. hydrophilia and were all fed the probiotic diet (and designated as probiotic treatment; T1). All the fishes were fed for four weeks at 3% of their biomass during the experimental period. Haematology and immunological assay of blood sample after the 14 th and 28 th post-infection period revealed that these parameters were better in the challenged fish (infected) maintained on the probiotic diet than those in the groups fed the non-probiotic diet. This study shows that dietary L. acidophilus is quite effective as an immunostimulant against Aeromonas hydrophila infection in H. longifilis broodstocks in routine hatchery management.
Probiotics are mono or mixed cultures of live organisms which improve the host’s health status and indigenous flora properties. The use of probiotics in enhancing growth and as replacement for synthetic growth promoters in animal health management and nutrition has been scientifically proven. This study was carried out to evaluate the intestinal flora of the African catfish, Clarias gariepinus for probiotic properties, develop suitable probiotic formulation/s from them; and evaluate the efficacy of the developed probiotic for promoting growth and health in the African catfish in comparison with a commercial probiotic, Mito yeast. Laboratory screening of the gut Microflora of cultured C. gariepinus using standard methods revealed Lactobacillus plantarum as a viable organism with probiotic properties. Lactobacillus plantarum (Accession no: KP 410238) was thereafter cultured, processed and included as supplement in the diet of C. gariepinus and evaluated for growth and health enhanceme...
Aquaculture Research, 2010
This study was carried out to evaluate the use of Lactobacillus acidophilus as a biocontrol agent against some common fish pathogenic bacteria (Staphylococcus xylosus, Aeromonas hydrophila gr.2 and Streptococcus agalactiae) in African catfish, Clarias gariepinus. Eight treatments were designed inclusive of 10 C. gariepinus juveniles (mean weight 190 g) per tank, each in triplicate. Four groups of fish were fed a diet supplemented with L. acidophilus, comprising about 3.01 × 107 colony-forming units per gram of diet (the probiotics diet), while the other four groups were fed a diet not supplemented with probiotics (the non-probiotics diet). In the first group, fish were injected with 1 mL physiological saline and fed the non-probiotic diet (non-probiotic control); in the second, third and fourth groups, fish were injected with 1 mL each of S. xylosus, A. hydrophila gr.2 and S. agalactiae, respectively, and were all fed the non-probiotic diet (designated as non-probiotic treatments; NPsx, NPah and NPsa respectively). In the fifth group, fish were injected with 1 mL physiological saline but fed the probiotic diet (probiotic control), while fish in the sixth, seventh and eighth groups were each injected with 1 mL of S. xylosus, A. hydrophila gr.2 and S. agalactiae, respectively, and were all fed the probiotic diet (and designated as probiotic treatments; Psx, Pah and Psa respectively). Blood samples were collected for haematology analysis, while samples of the liver and kidney were examined for pathohistology after 7 and 21 days of infection. The results showed that the haematology parameters, packed cell volume, haemoglobin, erythrocyte sedimentation rate, red blood cell, white blood cell, total serum protein, Mg2+, Ca2+, Cl−, glucose, cholesterol and total immunoglobulin concentrations and the pathohistology of the liver and kidney were better in the challenged fish (infected) maintained on the probiotic diet than those in the groups fed the non-probiotic diet. It is concluded, based on these results, that L. acidophilus is useful as a probiotic agent in C. gariepinus against these pathogenic bacteria (S. xylosus, A. hydrophila gr.2 and S. agalactiae).
Journal of Pure and Applied Microbiology
The effects of a probiotic Lactobacillus plantarum CR1T5, at various concentrations(0, 10 6 , 10 7 , 10 8 and 10 9 cfu g-1) in dietary supplements for Black Eared Catfish (Pangasius larnaudii) were investigated. Fish were randomly allocated into 5 treatments with 3 replications and reared for 60 days. The growth performance, non-specific immunity and disease resistance were determined at 15, 30, 45 and 60 days. The results revealed that at day 15 the fish received probiotic dietary supplements at 10 7 , 10 8 and 10 9 cfu g-1 had significantly higher Specific growth rate (SGR) and lower feed conversion ratio (FCR) than the fish received control diet. On the other hand, fish fed 10 6 cfu g-1 dietary supplement had significantly higher SGR and lower FCR at day 45. For immune parameters, the respiratory burst activity was significantly increased after 30 days. Probiotic diets at 10 8 and 10 9 cfu g-1 significantly improved complement activity after 15 days and significantly enhanced lysozyme activity after 45 days of feeding. All fish received probiotic dietary supplement displayed a significantly increased survival rate post-challenge with A. hydrophila. This study found that the optimum probiotic dietary supplement at 10 8 cfu g-1 had significantly improved growth performance, immune stimulation and disease resistance in P. larnaudii.
Advances in Biological Sciences Research, 2021
For 45 days, research was conducted to evaluate the administration of probiotic Bacillus NP5 to increase growth, body composition, and feed efficiency on catfish (Clarias sp.). This research was carried out with 3 different treatment of Bacillus NP5 probiotic doses (0, 0.5 and 1% probiotic) and 3 replications. The result showed that the value of the daily growth rate significantly showed the highest value (P<0.05) in 1% probiotic (11.32%), followed by 0.5% probiotic (9.20%) and control (8.20%). The value of protein and lipid composition was significantly in 1% probiotic (14.24%, 5.49%, respectively) than the other treatment. The administration of probiotic Bacillus NP5 not affect to water quality of catfish.
2019
The study was carried out to determine the influence of dietary supplementation of commercial probiotic, Bactofort ® on growth, haematological parameters and survival of Clariasgariepinus fingerlings raised in plastic tanks . The experiment was conducted for two months (61days) at the Fisheries laboratory, Department of Fisheries and Aquaculture, Federal University Dutsin-Ma. One hundred and twenty fingerlings of C. gariepinus with average weight of 11.3 g, were weighed and randomly distributed into four treatment groups consisting of twelve plastic tanks as follows: T0, which is the control group with no probiotic inclusion; T1, T2 and T3, representing 1, 2 and 3g of probiotic (Bactofort ® ) supplementation respectively. All treatments were in triplicates. Fish were fed to satiation two times daily, seven days a week (morning 8.00am and evening 5.00pm ). The growth performance (Final Mean Weight, Mean Weight Gain, Percentage Mean Weight Gain, and Specific Growth Rate) and nutrient...
Effects of probiotic as food additives on Nile catfish Clarias lazera
Egyptian Journal of Chemistry and Environmental Health, 2015
Comparison of the concentrations of serum nitric oxide; malonaldhyde; total protein and its electrophoretical profile in Nile catfish Clarias lazera after supplementation with probiotic for 45 days either 1 time/week (W 1) all over the experiment or 3 times/week for the 1 st two weeks then 1 time/week till the end of the experiment (W 3) were investigated. Probiotic supplementation affects on both serum nitric oxide and malonaldhyde. No effect of probiotic supplementation on serum total protein. Twelve fractions were resolved of which 10 and 11 were the maximum numbers that appeared with consistency in females and males respectively. Polymorphism appeared in both males and females of the initial; control; W 1 and W 3 groups. Probiotic feeding altered the relative density of resolved serum protein fractions. In both W 1 and W 3 groups, the 9 th fraction recorded higher values in both sexes, while fractions # 10 and 11 (in γ-globulin area) recorded lower values in both sexes. The relative densities of fractions # 5; 7 and 9-12 were significantly affected by probiotic supplementation. Our study indicated that probiotic supplementation to fish diet is not a long-term effect and preferred to be added periodically according to farm management.
Veterinary world/Veterinary World, 2024
Background and Aim: Catfish has a high economic value and is popular among consumers. To ensure well-stocked catfish stocks, good fisheries management must also be ensured. The high demand for catfish must be supplemented by preventive measures against pathogenic bacterial infections using probiotics with high potential for Lactobacillus casei and Bacillus subtilis. The aim of this study was to determine the effect of probiotic supplementation consisting of a combination of L. casei and B. subtilis probiotics on the growth, immune system, water quality, proximate value of feed, and body composition of catfish infected with Aeromonas hydrophila. Materials and Methods: This study used a completely randomized study with eight treatments and three replications. The manipulated factor was the probiotic concentration [0% (A), 0.5% (B), 10% (C), and 15% (D)] in groups of catfish infected and uninfected with A. hydrophila. Combination of B. subtilis, and L. casei that were used in a 1:1 ratio of 10 8 colony forming unit/mL. The study lasted for 42 days. On the 35 th day, A. hydrophila was infected by intramuscular injection into fish. The Statistical Package for the Social Sciences (SPSS) software version 23.0 (IBM SPSS Statistics) was used to analyze data on growth, immune system, and water quality. Results: Providing probiotics in feed can increase the nutritional value of feed based on proximate test results. There were significant differences in average daily gain (ADG), feed conversion ratio (FCR), and survival rate (SR) parameters in the group of catfish infected with A. hydrophila (p > 0.05); however, there were no significant differences in final body weight, specific growth rate (SGR), and percentage weight gain. Interleukin-1β (IL-1β) levels were significantly different between treatments C and D. The tumor necrosis factor (TNF) α parameters were significantly different between treatments A and C, whereas the phagocytic activity of treatment A was significantly different from that of treatment D. There was a significant difference (p > 0.05) in the growth parameters of SGR, ADG, and FCR in the group of fish that were not infected with A. hydrophila, with the best treatment being a probiotic concentration of 15%, but there was no significant difference in the SR parameters. IL-1β and TNF-α levels significantly differed between E and E0 (15% probiotics) but were not significantly different in terms of phagocytosis parameters. Conclusion: Based on the results of this study, it can be concluded that using a combination of probiotics L. casei and B. subtilis can improve the growth, immune system, water quality, proximate value of feed, and body composition of catfish infected with A. hydrophila.
Biodiversitas Journal of Biological Diversity, 2020
The purpose of this study was to investigate the effect of administration of the probiotic Bacillus NP5 in rearing media on water quality, growth, and disease resistance of African catfish. The study consisted of three different concentrations of probiotic Bacillus NP5-C: control, A: probiotic Bacillus NP5 of 1 x 10 9 CFU/mL, and B: probiotic Bacillus NP5 of 1 x 10 10 CFU/mL, on the rearing media of African catfish for 45 days. Based on the results, the lowest significant ammonia (P <0.05) was found in treatment A (1.91 ± 0.17 mg/L), then followed by B (2.12 ± 0.14 mg/L) while the highest was in the control (2.36 ± 0.19 mg/L). Treatment A also had a better immune response compared to other treatments shown with the highest significant number of leukocytes at 6.69 ± 0.17 x 10 4 cells/mm 3 and phagocyte activity at 65.19 ± 0.04%. Furthermore, treatment A significantly resulted in the best specific growth rate and feed conversion ratio (2.02 ± 0.07%.day-1 , 1.05 ± 0.07, respectively). The administration of probiotic Bacillus NP5 of 1 x 10 9 CFU/mL in rearing media significantly improved the ammonia value, growth, and resistance of African catfish to Aeromonas hydrophila infection.