Effects of the probiotic, Lactobacillus acidophilus , on the growth performance, haematology parameters and immunoglobulin concentration in African Catfish ( Clarias gariepinus , Burchell 1822) fingerling (original) (raw)
Advances in Biological Sciences Research, 2021
For 45 days, research was conducted to evaluate the administration of probiotic Bacillus NP5 to increase growth, body composition, and feed efficiency on catfish (Clarias sp.). This research was carried out with 3 different treatment of Bacillus NP5 probiotic doses (0, 0.5 and 1% probiotic) and 3 replications. The result showed that the value of the daily growth rate significantly showed the highest value (P<0.05) in 1% probiotic (11.32%), followed by 0.5% probiotic (9.20%) and control (8.20%). The value of protein and lipid composition was significantly in 1% probiotic (14.24%, 5.49%, respectively) than the other treatment. The administration of probiotic Bacillus NP5 not affect to water quality of catfish.
Journal of Pure and Applied Microbiology
The effects of a probiotic Lactobacillus plantarum CR1T5, at various concentrations(0, 10 6 , 10 7 , 10 8 and 10 9 cfu g-1) in dietary supplements for Black Eared Catfish (Pangasius larnaudii) were investigated. Fish were randomly allocated into 5 treatments with 3 replications and reared for 60 days. The growth performance, non-specific immunity and disease resistance were determined at 15, 30, 45 and 60 days. The results revealed that at day 15 the fish received probiotic dietary supplements at 10 7 , 10 8 and 10 9 cfu g-1 had significantly higher Specific growth rate (SGR) and lower feed conversion ratio (FCR) than the fish received control diet. On the other hand, fish fed 10 6 cfu g-1 dietary supplement had significantly higher SGR and lower FCR at day 45. For immune parameters, the respiratory burst activity was significantly increased after 30 days. Probiotic diets at 10 8 and 10 9 cfu g-1 significantly improved complement activity after 15 days and significantly enhanced lysozyme activity after 45 days of feeding. All fish received probiotic dietary supplement displayed a significantly increased survival rate post-challenge with A. hydrophila. This study found that the optimum probiotic dietary supplement at 10 8 cfu g-1 had significantly improved growth performance, immune stimulation and disease resistance in P. larnaudii.
Effects of probiotic as food additives on Nile catfish Clarias lazera
Egyptian Journal of Chemistry and Environmental Health, 2015
Comparison of the concentrations of serum nitric oxide; malonaldhyde; total protein and its electrophoretical profile in Nile catfish Clarias lazera after supplementation with probiotic for 45 days either 1 time/week (W 1) all over the experiment or 3 times/week for the 1 st two weeks then 1 time/week till the end of the experiment (W 3) were investigated. Probiotic supplementation affects on both serum nitric oxide and malonaldhyde. No effect of probiotic supplementation on serum total protein. Twelve fractions were resolved of which 10 and 11 were the maximum numbers that appeared with consistency in females and males respectively. Polymorphism appeared in both males and females of the initial; control; W 1 and W 3 groups. Probiotic feeding altered the relative density of resolved serum protein fractions. In both W 1 and W 3 groups, the 9 th fraction recorded higher values in both sexes, while fractions # 10 and 11 (in γ-globulin area) recorded lower values in both sexes. The relative densities of fractions # 5; 7 and 9-12 were significantly affected by probiotic supplementation. Our study indicated that probiotic supplementation to fish diet is not a long-term effect and preferred to be added periodically according to farm management.
Aceh journal of animal science, 2022
A 10-week feeding trial was carried out to evaluate strain-specific effects of Lactobacillus plantarum on blood profiles of African catfish Clarias gariepinus fingerlings. A catfish diet was formulated and divided into five portions (experimental diets). The first portion served as control (with no probiotic added to it, labeled as NP) while the second portion received 2.5g kg-1 multi-species commercial probiotics (CP). The third, fourth and fifth portions were the experimental diets that contained 2×10 9 cfu/g of L. plantarum strains of ascension numbers LC333558, LC333559 and LC333560, and labeled as LP58, LP59 and LP60, respectively. Fifteen apparently healthy C gariepinus fingerlings (4.86±0.59g) were stocked in an experimental tank of 70 litres capacity, while the experimental feed was fed to the fish in triplicate tanks. Similar rearing conditions were maintained in all experimental tanks. The results showed significant (p<0.05) difference between the fish fed control diet and probiotics supplementation diets in some of the haematological parameters examined. Probiotics supplementations did not affect total and differential leukocytes among different experimental groups. Except for RBC counts, fish groups fed with strains LB59 and LB60 had similar effects and appeared to have better haematological values than groups fed with LB58. Strains-specific effect of L. planatrum was also observed in creatinine levels among the experimental groups. Hence, it can be concluded that strains of L. plantarum affected some haematological parameters of C. gariepinus fingerlings positively, while dietary supplementation of L. plantarum LC333559 strain could be employed to improve haematological parameters of C. gariepinus fingerlings.
2019
The study was carried out to determine the influence of dietary supplementation of commercial probiotic, Bactofort ® on growth, haematological parameters and survival of Clariasgariepinus fingerlings raised in plastic tanks . The experiment was conducted for two months (61days) at the Fisheries laboratory, Department of Fisheries and Aquaculture, Federal University Dutsin-Ma. One hundred and twenty fingerlings of C. gariepinus with average weight of 11.3 g, were weighed and randomly distributed into four treatment groups consisting of twelve plastic tanks as follows: T0, which is the control group with no probiotic inclusion; T1, T2 and T3, representing 1, 2 and 3g of probiotic (Bactofort ® ) supplementation respectively. All treatments were in triplicates. Fish were fed to satiation two times daily, seven days a week (morning 8.00am and evening 5.00pm ). The growth performance (Final Mean Weight, Mean Weight Gain, Percentage Mean Weight Gain, and Specific Growth Rate) and nutrient...
Background The objective of the present study was to evaluate the growth performance and feed utilization of African catfish fed a commercial diet and Clarias gariepinus reared in the biofloc system enhanced with probiotic. Methods The treatment was the frequency of probiotic application into the cultured system, namely, 5-day interval, 10-day interval, and 15-day interval for 60 days of experiment. Biofloc culture was grown in an experiment tank (vol. 2000 L) by mixing the probiotic (sp.) 10 mL and molasses 200 mL per liter of Bacillus water. The fish was stocked into the biofloc system 7 days after cultured at stocking density of 1000 fish tank. The fish was fed a commercial diet that contains 38% crude protein, twice a day at satiation. The application of probiotic was reperformed after 5 days, 10 days, and 15 days after stocking. Results The study showed that the growth performance, survival, and feed utilization of African catfish were higher in the treatment at 5-day intervals over 60 days. The ANOVA test showed that the application frequency of probiotic into biofloc system of cultured media had the significant effect on the growth performance, survival rate, and feed utilization of African catfish. Conclusion The best growth performance and feed utilization were found at the application of probiotic into biofloc system at 5-day intervals over 60 days.
Aquaculture Research
The influence of dietary supplementation of Lactobacillus acidophilus, on growth performance, digestive enzyme activities, gut histomorphology and gut microflora were evaluated in juveniles striped catfish (Pangasianodon hypophthalmus). Five experimental diets were formulated by supplementing 0, 10 3 , 10 5 , 10 7 and 10 9 CFU/g L. acidophilus in fishmeal and casein-based semi-purified diet. Triplicate groups of striped catfish (21.69 ± 0.18 g) were stocked in 15 fiberglass tanks with stocking density of 25 individuals per tank and fed twice daily at 2.5% of the fish body weight for 12 weeks. Weight gain, specific growth rate, feed conversion ratio and the protein efficiency ratio were significantly higher in fish fed with 10 5 and 10 7 CFU/g L. acidophilus supplemented diets compared with the other treatment groups. Compared with the control and fish fed low (10 3 CFU/g) L. acidophilus supplementation, those fed with 10 5 and 10 7 CFU/g had significantly higher (p < 0.05) apparent protein digestibility. Inclusion of L. acidophilus at 10 7 CFU/g diet significantly increased amylase, protease and lipase activities. Microscopic analysis showed that the villi length in both the anterior and posterior gut and microvilli length in the posterior gut increased significantly in fish fed L. acidophilus supplementation at 10 5 and 10 7 CFU/g of diet. The fish fed L. acidophilus supplemented diets significantly increased the total lactic acid bacteria counts in the gut of striped catfish compared with the control-fed group. Based on gut histomorphology and growth performance, inclusion of L. acidophilus at 10 5 CFU/g appears to have the most positive effect on striped catfish farming.
Biodiversitas Journal of Biological Diversity, 2020
The purpose of this study was to investigate the effect of administration of the probiotic Bacillus NP5 in rearing media on water quality, growth, and disease resistance of African catfish. The study consisted of three different concentrations of probiotic Bacillus NP5-C: control, A: probiotic Bacillus NP5 of 1 x 10 9 CFU/mL, and B: probiotic Bacillus NP5 of 1 x 10 10 CFU/mL, on the rearing media of African catfish for 45 days. Based on the results, the lowest significant ammonia (P <0.05) was found in treatment A (1.91 ± 0.17 mg/L), then followed by B (2.12 ± 0.14 mg/L) while the highest was in the control (2.36 ± 0.19 mg/L). Treatment A also had a better immune response compared to other treatments shown with the highest significant number of leukocytes at 6.69 ± 0.17 x 10 4 cells/mm 3 and phagocyte activity at 65.19 ± 0.04%. Furthermore, treatment A significantly resulted in the best specific growth rate and feed conversion ratio (2.02 ± 0.07%.day-1 , 1.05 ± 0.07, respectively). The administration of probiotic Bacillus NP5 of 1 x 10 9 CFU/mL in rearing media significantly improved the ammonia value, growth, and resistance of African catfish to Aeromonas hydrophila infection.
Use of Probiotics as First Feed of Larval African Catfish Clarias gariepinus (Burchell 1822
African catfish larvae do not have fully developed digestive system at onset of first exogenous feeding. Live feed like artemia have been used more than dry diets but is also costly. The gut microbiota may be influential in larval diet utilization. We therefore made three larval feed from two commercial probiotics and tested their growth and nutritional effects on first feeding hatchlings (larvae) of African catfish. The three diets were labeled as feed 1 (F1) made up of mixtures of mixtures of Lactobacillus acidophilus, Bacillus subtilis and Lactobacillus bugaricus, feed 2 (F2) was made up of 100% Saccharomyces cerevisiae and feed 3 (F3) was made up of 50% Lactobacillus mixtures and 50% Saccharomyces cerevisiae. The control diet F4 was decapsulated artemia. African catfish hatchlings were produced by artificial dry fertilization of brooders maintained in our lab. First feeding hatchlings 48 hours post fertilization (48 h pf) were stocked in well aerated 15l plastic aquariums at density of 100 larvae aquarium-1. There were three replicate aquariums per treatment feed. The photoperiod of the larval rearing system was 12 h D: 12 h L and the light intensity were maintained at 8 lux using black nylon coverings. The hatchlings were fed to satiation Original Research Article
F1000Research, 2017
Background The objective of the present study was to evaluate the growth performance and feed utilization of African catfish Clarias gariepinus fed a commercial diet and reared in the biofloc system enhanced with probiotic. Methods The treatment was the frequency of probiotic application into the cultured system, namely, 5-day interval, 10-day interval, and 15-day interval for 60 days of experiment. Biofloc culture was grown in an experiment tank (vol. 2000 L) by mixing the probiotic (Bacillus sp.) 10 mL and molasses 200 mL per liter of water. The fish was stocked into the biofloc system 7 days after cultured at stocking density of 1000 fish tank-1. The fish was fed a commercial diet that contains 38% crude protein, twice a day at satiation. The application of probiotic was reperformed after 5 days, 10 days, and 15 days after stocking. Results The study showed that the growth performance, survival, and feed utilization of African catfish were higher in the treatment at 5-day inter...