Interaction of a Cationic Dye “Nile Blue A”with Bile Salts (original) (raw)

Interaction of a reactive dye with serum albumins and with aminoacids: the dye as a chiral label

Dyes and pigments, 2002

HSA and BSA, human and bovine serum albumins, react with a reactive dye, C.I. Reactive Blue 29 (levafix brillant blue EB) giving adducts containing two dye molecules per protein molecule. While cysteine was able to react with the dye, the cysteine(s) present in the proteins was not. The dye-protein adducts reveal strong circular dichroism in the 300-720 nm spectral region where only absorption bands of the achiral dye molecule are active. The intensity of these bands depends on the conformation of the protein: particularly the 530-720 nm band. On tuning the denaturation degree by means of SDS addition and removal, the intensity of this band varies between zero and a maximum when the protein is in fully denaturated and native conditions, respectively. This behavior evidences a chiral label function of the dye that can be useful in studying processes regarding proteins.

Green toxicology and chemistry: Hand in glove

Toxicology Letters, 2016

On the territory of modern Uzbekistan there are a lot of historical and architectural monuments of different eras -this magnificent, unique in its beauty palaces, mausoleums and minarets. Many of them are in the treasury of human values and are included in the list of UNESCO "World Heritage" and are protected by the international community. Their monumentality, beauty and reliability have always attracted many scientists to study the secrets of the construction of such structures on difficult soil and seismic conditions of construction sites in the region. This article presents the research design solutions, engineering-geological conditions and the results of surveys and the minaret of the mosque Kalon in Bukhara