Antimicrobial and Toxicological Studies of Ricinodendron Heudelotii (BAILL.) (original) (raw)

Acute toxicity studies, antioxidant and in vitro antibacterial activities of extract from the barks of Ricinodendron heudoletti (Euphorbiaceae)

Journal of Pharmacognosy and Phytotherapy

Many bacteria are involved in infectious diseases. Most of these bacteria become resistant to the most commonly used synthetic drugs. In Cameroon, natural substance seem to be an alternative to this problem. Thus the aim of this research was to investigate the acute toxicity, antioxidant activities and the in vitro antibacterial of the methanol extract of Ricinodendron heudelotii (Euphorbiaceae) against twelve pathogenic bacteria involved in infectious diseases. The major bioactive components were also screened. The antibacterial activity of the extract was investigated against 12 strains including 10 Gram-and 2 Gram+ bacteria by disc diffusion method and micro dilution method, followed by another agar disc diffusion for the determination of inhibition diameters, the minimum inhibitory concentration (MICs) and the minimum bactericidal concentration (MBC), respectively. 2,2-Diphényl-1-Picrylhydrazyl (DPPH) assay was used to evaluate antiradical activity. The acute toxicity study was ...

In Vitro Analysis, Secondary Metabolite Screening and GC-MS Profile of Ricinodendron Heudelotii(Muh.Arg) Essential Oil Extracts Against Selected Multiple Drug Resistance Clinical Isolates

International Journal of Research and Innovation in Applied Science (IJRIAS) |

Essential oils are valuable natural products used as raw materials in many fields and can be define as a volatile and liquid aroma compounds from natural sources usually plants. They may be found in different parts of plant, some could be leaves, seed, flower, peel, and Stem bark. This study aims at screening the antimicrobial property from the essential oil extracted from Ricinodendron huedelotii fresh seed and stem bark using soxhlet method with two distinct solvents(N-hexane and ethyl acetate). Antibacterial activity was carried out using well agar diffusion technique against multiple resistance clinical isolates. The Nhexane oil extracts from Ricinodendron heudelotii seed revealed Bacillus subtilis and Pseudomonas aeruginosa having the widest zone of inhibition (15.0mm) at 100mg/ml while the least zone of inhibition (1.0mm) was recorded for Escherichia coliat 12.5mg/ml with essential oil ethyl acetate extracts from Ricinodendron huedelotiistem bark. Phytochemical analysis of the plant showed the present of active components such as cardiac glycoside, steroid, flavonoid, phenol, alkaloid, reducing sugar,tannin, saponin, pyrrolizidine, terpenoid and volatile oil. The present of these components enhances the effectiveness of plant essential oilin treating various diseases and helped to act as an antibacterial agent. Essential oil extracted from Ricinodendrn huedelotii seed using two solvents was further analysed by gas chromatography – mass spectrophotometer. The main constituents were Oxycycloheptadec 8-en-one (20.48%), Pentadecanoic acid (11.95%), n-propyl 9,12-Octadecadienoic acid (6.40%), Octadesetrienoic acid ethyl ester (10.30%), Ascorbic acid(11.27%), Gamolenic acid (17.74%), Linolenic acid, methyl ester (6.74%) and Eicososatrienoic acid (8.94%).Those components aid the antibacterial activities of essential oil from the seed and stem bark of Ricinodendron heudelotii. The results obtained suggest that the Ricinodendron heudelotii essential oil can serve as an effective antibacterial agent.

Bioprospective Screening of Ricinodendron Heudelotii Seeds

Submit Manuscript | http://medcraveonline.com This study therefore screens for the presence (qualitative) and concentration (quantitative) phytochemical constituents of the extract of the dried Ricinodendron heudelotii seed, aimed at enhancing the therapeutic value and its use in pharmaceutical industries.

Phytochemical Screening and Antibacterial Activity on Ricinus Communis L

2011

PG and Research Centre, Department of Botany, S.T. Hindu College, Nagercoil, Kanyakumari District, Tamil Nadu, India. Email* surejkensa@gmail.com The medicinal plants represent an enormous reservoir of potential microbial compounds that could be useful as an alternative to synthetic microbicides and are being used to develop drugs. In the present study, the leaf, stem and root powder extracts of Ricinus communis L. (Euphorbiaceae) were tested against three different bacteria by disc diffusion method. It was found that acetone and hexane extract possess good zone of inhibition where as ethanolic extract having antibacterial activity only on higher concentration. © 2011 LifeSciFeed Ventures

Cytotoxic Effects of Compounds Isolated from Ricinodendron heudelotii

Molecules, 2019

This study was designed to explore the in vitro anticancer effects of the bioactive compounds isolated from Ricinodendron heudelotii on selected cancer cell lines. The leaves of the plant were extracted with ethanol and partitioned in sequence with petroleum ether, ethyl acetate, and n-butanol. The ethyl acetate fraction was phytochemically studied using thin layer chromatography (TLC) and column chromatography (CC). Structural elucidation of pure compounds obtained from the ethyl acetate fraction was done using mass spectra, 1H-NMR, and 13C-NMR analysis. The isolated compounds were subsequently screened using five different cancer cell lines: HL-60, SMMC-7721, A-549, MCF-7, SW-480, and normal lung epithelial cell line, BEAS-2B, to assess their cytotoxic effects. Nine compounds were isolated and structurally elucidated as gallic acid, gallic acid ethyl ester, corilagin, quercetin-3-O-rhamnoside, myricetin-3-O-rhamnoside, 1,4,6-tri-O-galloyl glucose, 3,4,6-tri-O-galloyl glucose, 1,2,...

Evaluation of phytochemicals and in vitro antimicrobial activity of aqueous and ethanolic extract from seeds of Ricinus communis Linn

The current study has been carried out to evaluate phytochemical components from aqueous and ethanolic extract of seeds of Ricinus communis and also deals with its in vitro antimicrobial activity. The aqueous extract showed presence of steroids, tannin, saponin, alkaloids, phytosterol and cardial glycosides while ethanolic extract showed presence of diterpenes, steroids, flavonoids, tannin, coumarin, phenols, alkaloids and saponin. The in vitro antimicrobial activity of aqueous and ethanolic extract of seeds of Ricinus communis were tested against Bacillus subtilis, Salmonella typhimurium, Peudomonas auruginosa, Staphylococcus aureus, Proteus vulgaris, Aspergillus niger and Penicillium chrysogenum by agar well diffusion method. The aqueous extract of the sample showed significant range of inhibitory effect against Salmonella typhimurium and Peudomonas auruginosa, however the ethanolic extract is more effective and showed inhibitory effect against all the bacteria. The GCMS data revealed that novel bioactive components are present in both the extract from seeds of Ricinus communis. The aqueous and ethanolic extract doesn’t showed antifungal activity against Aspergillus niger and Penicillium chrysogenum. Thus, these results confirms the presence of active antibacterial compounds in seeds of Ricinus communis, might be used as a source of antibiotics.

Essential oil of the leaves of Ricinus communis L.: In vitro cytotoxicity and antimicrobial properties

Lipids in Health and Disease, 2012

Background The aim of the present study was to appraise the antimicrobial activity of Ricinus communis L. essential oil against different pathogenic microorganisms and the cytotoxic activity against HeLa cell lines. Methods The agar disk diffusion method was used to study the antibacterial activity of Ricinus communis L. essential oil against 12 bacterial and 4 fungi strains. The disc diameters of zone of inhibition (DD), the minimum inhibitory concentrations (MIC) and the concentration inhibiting 50% (IC50) were investigated to characterize the antimicrobial activities of this essential oil. The in vitro cytotoxicity of Ricinus communis L. essential oil was examined using a modified MTT assay; the viability and the IC50 were used to evaluate this test. Results The essential oil from the leaves of Ricinus communis L. was analyzed by GC–MS and bioassays were carried out. Five constituents of the oil were identified by GC–MS. The antimicrobial activity of the oil was investigated in o...

Ricinodendron heudelotii Extract Strongly Protects Mice againstAcetaminophen-Induced Liver Injury

2020

Ricinodendron heudelotii (Baill.) Pierre ex Pax. (Euphorbiaceae) is used in Cameroonian traditional medicine to cure liver diseases, malaria, and stomach pain. The present study investigates the effect of R. heudelotii stem bark aqueous extract on acetaminopheninduced acute liver injury in BALB/c mice. R. heudelotii stem bark aqueous extract (100 and 200 mg/kg, p.o.), distilled water and ascorbic acid (50 mg/kg, p.o.) were administered to mice 1 and 12h before liver injury induction by acetaminophen (500 mg/kg, p.o.). Mice were sacrificed 6h later. Biochemical and histological analyses were used to assess liver injury. Acetaminophen administration resulted in significant (P < 0.01) increase of liver relative weight, liver enzymes [aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP)] serum activities, nitrite and malondialdehyde (MDA) liver content. R. heudelotii extract significantly (P < 0.05) reduced ALT and ALP serum activity, MDA...

Anti-inflammatory and antioxidant activities of fractions and compound from Ricinodendron heudelotii (Baill.)

Heliyon

Medicinal plants have been documented over the years to play vital role in promoting human health. The study evaluated the anti-inflammatory and anti-oxidant activities of different fractions and isolated compound from Ricinodendron heudelotii leaves. The leaves of Ricinodendron heudelotii were extracted with ethanol and further partitioned sequentially using petroleum ether, ethylacetate and butanol. Bioassay-guided fractionation of the ethylacetate fraction was done using repeated column chromatographic technique while the structural elucidation of pure compound was carried out using mass spectra, 13 C and 1 H NMR analyses. Antioxidant potential of the fractions and isolated compound were evaluated with 2,2-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays and anti-inflammatory effect of fractions was measured by their inhibitory potency on nitric oxide (NO). Corilagin, an amorphous tannin was isolated and structurally elucidated. Corilagin showed scavenging effect against ABTS and DPPH radicals which vary in a dose dependent manner. It also showed an antioxidant potential with IC 50 value of 0.003 mg/mL comparable to vitamin C 0.001 mg/mL) used as standard. The butanol and ethylacetate fractions exhibited significant (p < 0.05) NO inhibition of 60 and 69% respectively after treatment of RAW 264.7 macrophages with lipopolysaccharide. These results demonstrated the role of isolated corilagin as a promising potent antioxidant while the ethylacetate and butanol fractions suppressed the expression of an inflammation mediator by inhibiting nitric oxide.

Antimicrobial potential of Ricinus communis leaf extracts in different solvents against pathogenic bacterial and fungal strains

Asian Pacific journal of tropical biomedicine, 2012

To investigate the in vitro antimicrobial activities of the leaf extract in different solvents viz., methanol, ethanol and water extracts of the selected plant Ricinus communis. Agar well diffusion method and agar tube dilution method were carried out to perform the antibacterial and antifungal activity of methanol, ethanol and aqueous extracts. Methanol leaf extracts were found to be more active against Gram positive bacteria (Bacillus subtilis: ATCC 6059 and Staphylococcus aureus: ATCC 6538) as well as Gram negative bacteria (Pseudomonas aeruginosa: ATCC 7221 and Klebsiella pneumoniae) than ethanol and aqueous leaf extracts. Antifungal activity of methanol and aqueous leaf extracts were also carried out against selected fungal strains as Aspergillus fumigatus and Aspergillus flavus. Methanolic as well as aqueous leaf extracts of Ricinus communis were effective in inhibiting the fungal growth. The efficient antibacterial and antifungal activity of Ricinus communis from the present ...