DETERMINING THE PRESENCE OF THE FIG MOSAIC VIRUS (FMV) IN THREE VARIETIES OF Ficus carica L. IN COSTA RICA (original) (raw)
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2019
The study aimed at diagnostic FMV virus from infected Ficus carica from some Saladin nurseries based on the technique of Reverse Transcription RT-PCR. A band 300 bp was given. The result showed FMV similar to that found in Costa Rica, Iran, Lurstan, Japan, and also gave a band of 367pb of FMV RNA1 and the absence of other species. FLMAV-1 isolation was detected in fig leaf with a 352pb band and is similar in some countries. The study also included the use of Bacillus subtilis, Spirulina platensis, Ganoderma lucidum, (G. lucidum +S. platensis), (S. platensis+B. subtilis), (G. lucidum+B. subtilis). The treatment of B. subtilis + S. platensis for yellow and black to study the percentage of the severity of the effect of the injury which is the lowest and reached 23.0% compared with a control treatment, which gave 99.6%. The results of chlorophyll the treatment of mushrooms with moss (G + Sp) with a value of Spad 43.15 which is the highest compared to the treatment of control amounted to...
2019
The study aimed at diagnostic FMV virus from infected Ficus carica from some Saladin nurseries based on the technique of Reverse Transcription RT-PCR. A band 300 bp was given. The result showed FMV similar to that found in Costa Rica, Iran, Lurstan, Japan, and also gave a band of 367pb of FMV RNA1 and the absence of other species. FLMAV-1 isolation was detected in fig leaf with a 352pb band and is similar in some countries. The study also included the use of Bacillus subtilis, Spirulina platensis, Ganoderma lucidum, (G. lucidum +S. platensis), (S. platensis+B. subtilis), (G. lucidum+B. subtilis). The treatment of B. subtilis + S. platensis for yellow and black to study the percentage of the severity of the effect of the injury which is the lowest and reached 23.0% compared with a control treatment, which gave 99.6%. The results of chlorophyll the treatment of mushrooms with moss (G + Sp) with a value of Spad 43.15 which is the highest compared to the treatment of control amounted to 30.77 Spad.
The plant pathology journal, 2017
Fig mosaic disease (FMD) is a viral disease that spreads in all Tunisian fig (Ficus carica L.) orchards. RT-PCR technique was applied to leaf samples of 29 fig accessions of 15 fig varieties from the fig germplasm collection of High Agronomic Institute (I.S.A) of Chatt-Mariem, to detect viruses associated to FMD. Analysis results show that 65.5% of the accessions (19/29) and 80.0% (12/15) of the fig varieties are infected by FMD-associated viruses. From all fig accessions, 41.4% of them are with single infection (one virus) and 24.1% are with multi-infections (2 virus and more). Viruses infecting fig leaf samples are Fig mosaic virus (FMV) (20.7%), Fig milde-mottle-associated virus (FMMaV) (17.25%), Fig fleck associated virus (FFkaV) (3.45%), and Fig cryptic virus (FCV) (55.17%). A reliable protocol for FCV and FMMaV elimination from 4 local fig varieties Zidi (ZDI), Soltani (SNI), Bither Abiadh (BA), and Assafri (ASF) via in vitro culture of 3 meristem sizes was established and opt...
A virus with filamentous particles ca. 700 nm long, denoted Fig latent virus 1 (FLV-1) is widespread in Apulian (southern Italy) fig orchards, in trees showing or not mosaic symptoms and in symptomless seedlings. The virus was transmitted by sap inoculation to a very restricted range of herbaceous hosts without inducing apparent symptoms and was transmitted through fig seeds to a very high percentage (80 to 100 %). It was successfully purified from root tissues of infected figs. A virus-specific antiserum raised in rabbits, proved useful for its detection in fig leaf dips by immunosorbent electron microscopy (ISEM), Western Blot, dot immuno-binding (DIBA), ELISA. The viral genome structure resembles that of members of the genus Trichovirus in the family Flexiviridae.
Prevalence and genetic diversity of fig mosaic virus isolates infecting fig tree in Iran
Acta virologica, 2014
Commercial and outdoor fig orchards in four Iranian provinces were surveyed for the incidence of fig mosaic virus (FMV), fig leaf mottle associated virus 2 (FLMaV-2) and fig mild mottle associated virus (FMMaV) from March 2011 to October 2012. A total of 350 asymptomatic and symptomatic fig samples were collected and tested by dot-immunobinding assay (DIBA) for the fig mosaic disease (FMD) using a polyclonal antiserum. According to DIBA results, FMD was present in 73% of the collected symptomatic samples from all visited regions. Samples with positive reactions in DIBA were then analyzed by RT-PCR using with specific primers. PCR results showed that about 14.8% of the FMD-positive samples from three inspected provinces are infected with at least one virus. FMV was the most widely spread virus (14%) followed by FLMaV-2 (1.5%), whereas FMMaV was not found. Phylogenetic analysis of the glycoprotein nucleotide and amino acid sequences of known FMV isolates showed two independent groups ...
A virus with filamentous particles ca. 700 nm long, denoted Fig latent virus 1 (FLV-1) is widespread in Western Mecca region, Kingdom of Saudi Arabia (KSA) fig orchards. RT-PCR assay was conducted on 80 fig samples collected from four fig-growing provinces including Fatima, Khulais, Rabigh and Al Shifa valleys which consisted of leaf tissues gathered from Capri fig and common fig to investigate the presence of FLV-1. Results showed that the virus was present in the survey areas with different levels of infections. The infection rate reached 33.75%, with a peak of 35% in Alshifa and Fatima valley provinces followed by 40% and 25% in Rabigh and Khulais valley provinces respectively.
Molecular Identification of Fig Cryptic Virus and Fig Fleck-Associated Virus in Turkey
2017
Recently, several new viruses infecting fig trees were identified. To assess the presence, distribution and genetic diversity of Fig cryptic virus (FCV) and Fig fleck-associated virus (FFkaV) in fig trees of Turkey, a total of 65 fig samples, which show yellowing, chlorotic, necrotic spots and vein clearing symptoms, were collected from Aegean and Mediterranean regions, which are the most important fig growing regions of Turkey, in spring 2014 and tested by molecular analysis. After cDNA synthesis, FCV and FFkaV specific primer sets of RNA dependent RNA polymerase (RdRp) regions were used for reverse transcription-polymerase chain reaction (RT-PCR) analysis and the PCR products were directly sequenced. The obtained sequences were analyzed and nucleotide sequence analysis confirmed the FCV and FFkaV infections. According to the results, some of the fig trees are infected in Turkey by FCV and FFkaV with an incidence of 20 % and 9.2 %, respectively. BLAST analysis of both FCV and FFkaV...