Frequency of antitoxoplasma antibodies in patients with ocular pathology (original) (raw)
Related papers
2019
Toxoplasma gondii causes a vision-threatening parasitic disease (ocular toxoplasmosis) which has been associated with posterior uveitis worldwide both in immunosuppressed and immunocompetent individuals. This study investigated the sero-prevalence of anti-Toxoplasma gondii antibodies among undergraduate Students of Babcock University, Ilishan-Remo, Ogun State. The serum samples of 150 participants (75 males and 75 females, aged 16-36 years) with history of eye infection were randomly collected and screened using a one-step Bio-check Toxo IgM/IgG Rapid Antibody Test Cassette (Blue Cross Bio-Medical Co., Ltd, Beijing, China). The demographic and clinical information of the participants were also collected using a structured questionnaire. The outcome of the study shows that out of the 150 participants screened, 3 (2.0%) participants tested positive for anti-Toxoplasma gondii IgG antibody only, 1 (0.7%) tested positive for anti-Toxoplasma gondii IgM antibody only, while 1 (0.7%) person...
Memórias do Instituto …, 2000
We studied the frequency of specific anti-Toxoplasma IgM, IgA and IgE antibodies in serum of 28 immunocompetent Colombian patients, selected by ophthalmologists and with lesions that were compatible with ocular toxoplasmosis. Patients were classified in three groups: (i) group 1 consisted of ten patients with a first episode; (ii) group 2, with seven patients with a recurrence and (iii) group 3, consisted of eleven patients with chronic chorioretinal lesion without uveitis. We found that 10/28 (35%) of Colombian patients with ocular toxoplasmosis possessed at least one serological marker for Toxoplasma infection different from IgG. In group 1 (first episode), we found simultaneous presence of specific IgM plus IgA plus IgE in 1/10 (10%). In group 2 (recurrences) in 1/7 (14%) we found IgM and IgA test positives and in 1/7 (14%) we found IgM and IgE tests positives. In group 3 (toxoplasmic chorioretinal scar) the IgA serological test was positive in 2/11 (18%). These results show that serum IgM or IgA or IgE can be present during recurrences.
Serology in ocular toxoplasmosis
British Journal of Ophthalmology, 1986
The diagnostic value of toxoplasma serology in ocular disease was evaluated in the following groups of patients: (I) uveitis cases of various causes (n=291); (II) consecutive posterior and panuveitis patients (n=60); (III) patients with definite congenital and ocular toxoplasmosis (n=8); (IV) cases of clinical ocular toxoplasmosis (n=25); and control patients with uveitis of nontoxoplasma origin (n= 12). No relation was observed between the level of the dye test titres and the diagnosis of ocular toxoplasmosis (groups I and II). During the active stages of the disease no typical change of the titres occurred in several longitudinally studied patients with toxoplasmosis. In group III one case was discovered to be negative by the dye test despite active ocular disease; however, IgG antibodies against toxoplasma were detected by the ELISA technique. In group IV, which was investigated by the ELISA technique, 100% of the toxoplasmosis patients were positive for IgG versus 58% of the control patients. Circulating immune complexes containing IgG and toxoplasma antigen were detected in seven of 25 toxoplasmosis patients (28%) and in two of 12 control patients (16%). Our study shows that the definite diagnosis of ocular toxoplasmosis or its exclusion by serological means only is not yet feasible. The possible superiority of the ELISA test to the dye test warrants further investigation.
Journal of Clinical Microbiology, 1999
Toxoplasma chorioretinitis result from congenital infection. Patients are often asymptomatic during life, with a peak incidence of symptomatic illness in the second and third decades of life. Diagnosis is mainly supported by ophthalmological examination and a good response to installed therapy. However, establishment of a diagnosis by ophthalmological examination alone can be difficult in some cases. To determine the diagnostic value of PCR for the detection of T. gondii, 56 blood and 56 aqueous humor samples from 56 immunocompetent patients were examined. Fifteen patients with a diagnosis of ocular toxoplasmosis had increased serum anti-T. gondii immunoglobulin G levels but were negative for anti-T. gondii immunoglobulin M (group 1), and 41 patients were used as controls (group 2). Samples were taken before antiparasitic therapy was initiated, and only one blood sample and one aqueous humor sample were obtained for each patient. Single nested PCRs and Southern blot hybridization were performed with DNA extracted from these samples. The results obtained showed sensitivity and specificity values of 53.3 and 83%, respectively. Interestingly, among all patients with ocular toxoplasmosis, a positive PCR result with the aqueous humor sample was accompanied by a positive PCR result with the blood sample. This result suggests that ocular toxoplasmosis should not be considered a local event, as PCR testing of blood samples from patients with ocular toxoplasmosis yielded the same result as PCR testing of aqueous humor samples. PCR testing may be useful for discriminating between ocular toxoplasmosis and other ocular diseases, and also can avoid the problems associated with ocular puncture.
International Journal of Health Sciences (IJHS), 2022
Toxoplasma gondii is an intracellular protozoan parasite that causes ocular toxoplasmosis with most complications such as developing eye lesions and impaired vision. During the period from October 2021 to April 2022, a total of 174 blood samples were taken from ocular infected patients whom visited Shahid Dr. Aso Hospital in Sulaimani City, Iraq. These samples were tested serologically for detection of anti-Toxoplasma gondii antibodies (IgG and IgM) with both imunochromatograhpy (rapid test) and ELISA. The serological test demonstrated that 63 IgG (36.2%) and 4 IgM (2.2%) were positive by rapid test imunochromatograhpy. While, 80 IgG (45.9%) and 2 IgM (1.1%) were positive by ELISA. Most of the infections (58.75%) were between 50-69 years old. All patients (80) were examined for intraocular toxoplasmosis lesion, only 10 patients (12.5%) including 2 males (2.5%) and 8 females (10%) had ocular toxoplasmosis lesions. Most of the lesions were scars (old type) while, in some patients they were active lesions. The types of lesions, numbers per each eye, lesion diameter and comparison with the IgG and IgM titers were demonstrated. The present study indicates that ELISA diagnoses are highly sensitive to detect T. gondii than rapid test. In addition, the ocular toxoplasmosis lesions were more prevalent in the elderly and the number of the lesions increased with the age among ocular infected patients.
An age-adjusted seroprevalence study of Toxoplasma antibody in a Malaysian ophthalmology unit
Transactions of the Royal Society of Tropical Medicine and Hygiene, 2012
Toxoplasma gondii is a public health risk in developing countries, especially those located in the tropics. Widespread infection may inflict a substantial burden on state resources, as patients can develop severe neurological defects and ocular diseases that result in lifelong loss of economic independence. We tested sera for IgG antibody from 493 eye patients in Malaysia. Overall age-adjusted seroprevalence was estimated to be 25% (95% CI: [21%, 29%]). We found approximately equal age-adjusted seroprevalence in Chinese (31%; 95% CI: [25%, 38%]) and Malays (29%; 95% CI: [21%, 36%]), followed by Indians (19%; 95% CI: [13%, 25%]). A logistic regression of the odds for T. gondii seroprevalence against age, gender, ethnicity and the occurrence of six types of ocular diseases showed that only age and ethnicity were significant predictors. The odds for T. gondii seroprevalence were 2.7 (95% CI for OR: [1.9, 4.0]) times higher for a patient twice as old as the other, with ethnicity held constant. In Malays, we estimated the odds for T. gondii seroprevalence to be 2.9 (95% CI for OR: [1.8, 4.5]) times higher compared to non-Malays, with age held constant. Previous studies of T. gondii seroprevalence in Malaysia did not explicitly adjust for age, rendering comparisons difficult. Our study highlights the need to adopt a more rigorous epidemiological approach in monitoring T. gondii seroprevalence in Malaysia.
Serological investigation of ocular toxoplasmosis
British Journal of Ophthalmology, 1991
The limitations of serological assessment in toxoplasma infection of the eye are well recognised, but the predictive value of clinical examination is not defined. We undertook a prospective investigation into the role of clinical examination and of serological findings in cases of suspected toxoplasma infection of the eye by means of the dye test and multiple IgM assays. Seventy-four cases of retinal disease and 202 control patients were studied. Patients with retinal disease had a significantly higher incidence of toxoplasma seropositivity than the control group. This was because some patients with retinal disease had acquired the infection congenitally. Half the patients investigated for toxoplasmosis were seronegative. Possible explanations for these findings included misdiagnosis, clinical uncertainty, or, the use of serology testing in the confirmation of other diseases. An excess of IgM reactivity among the retinal disease group may indicate low level immunoglobulin-M production associated with an acute exacerbation of ocular toxoplasmosis. There is a need to consider invasive procedures in cases of ocular infection and for novel techniques to aid the diagnosis of toxoplasma retinochoroiditis.
Avicenna Journal of Clinical Microbiology and Infection, 2016
Background: Toxoplasmosis is one of the most widespread parasitic infections worldwide. The infection is mostly benign, although severe disease can be expected in immunocompromised/suppressed individuals and infants who are infected during pregnancy. Objectives: The aim of the present study was to determine the anti-Toxoplasma IgG and IgM seroprevalence in subjects who were referred to the Central Laboratory in Tabriz, Iran, for the diagnosis of toxoplasmosis. Methods: This retrospective cross-sectional study was carried out among 6393 individuals who were referred to the central laboratory, Tabriz University of Medical Sciences, Tabriz, Iran, from March 2013 to January 2014. The subjects were referred from different areas of East Azerbaijan province. The quantitative determination of anti-Toxoplasma IgG and IgM was performed using an antibody capture chemiluminescence immunoassay. The other available variables, including sex and age, were also recorded and analyzed. Results: In total, 1910 (29.87%) out of 6393 subjects were found to be infected with Toxoplasma gondii. In terms of the seropositivity, 1871 (32.42%) out of 5770 and 128 (2.14%) out of 5965 subjects were anti-Toxoplasma IgG and IgM seropositive, respectively. In addition, 103 (1.69%) out of 5828 subjects were equivocal with regard to anti-Toxoplasma IgM, while 57 (0.97%) out of 6068 individuals were equivocal with regard to anti-Toxoplasma IgG. The mean concentration of anti-Toxoplasma IgM was observed to be higher in male subjects than in females (P = 0.016). Conclusions: Based on the findings of the present study, the frequency of Toxoplasma infection is high in the studied population, although it is fairly close to the findings of reports from the other areas of Iran.
Seroepidemiologic evaluation of anti-toxoplasma antibodies among women in north of Iran
2007
This study is a cross sectional and descriptive study. Sample population was the women referred for marriage consultation to Gorgan 1.1arriage Consultation Center in 2004. The prevalence of toxoplasma antibodies (IgG, IgM) was determined by ELISA method. Blood samples were collected randomly from 300 women referred to the center for consultation. They were transferred to the laboratory for antibodies determination. Gorgan located in north of Iran and south east of Caspian sea. The results showed that the general prevalence to the positive cases based on high titer of IgG was 48.3% and of IgM was 11. 7%. There was not any relation between positive cases and age, education, place of residence, job, keeping domestic animals (except cat) vegetable consumption and wash-up, but there was a relation between the positive cases of IgM and keeping cat at home (PV<0.025). More over 51.7% of pregnant women in Gorgan were seronegative and the were prone to acute toxoplasmosis during their pregnancy. The results of this study confirm that the determination of the diagnostic toxoplasmosis is an necessary test during pregnancy.