Microbiological Quality and Antibiotic Susceptibility of E. coli Isolated from Agricultural Water in Gyeonggi and Gangwon Provinces (original) (raw)
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The Open Nutraceuticals Journal, 2012
The purpose of this study was to isolate cellulolytic and hemicellulolytic anaerobic bacteria inhabiting from gut of ruminants and investigate their hydrolytic enzyme activities. Extracellular CMCase activities of H-strains isolated from the rumen of a Holstein dairy cow were higher than those of D-and DC-strains from the rumen and large intestine of Korean spotted deer. Most isolated bacteria utilized more efficiently Dehority's artificial medium containing starch, glucose and cellobiose (DAS) than those in Dehority's artificial medium containing cellulose only (DAC). The results of biochemical reactions and sugar fermentation indicated that the isolated bacteria belong to one of bacterial strains of Peptostreptococcus spp., Bifidobacterium spp., Prevotela ruminicola/buccae, Clostridium beijer/butyricum and Streptococcus intermedis which are not highly cellulolytic. Activities of Avicelase, xylanase, β-D-glucosidase, α-L-arabinofuranosidase and β-xylosidase of the isolated anaerobic bacteria in DAS were higher than those in DAC. In conclusion, the results indicated the higher enzyme activities of the isolated strains cultured in DAS medium were mainly caused by their specific carbohydrate utilization for enzyme production and growth rate. The highly cellulolytic bacteria were not isolated in the present experiment. Thus further research is required to investigate characteristics of gut bacteria from Korean spotted deer.
Analisis Tingkat Cemaran Bakteri Coliform DI Sungai Batang Masumai Kabupaten Merangin
2020
To examine the optimal temperature, light intensity, and shell-type for shell-living conchocelis production, we tested the shell infiltration of free-living conchocelis fragments under various environmental conditions. Under a combination of various temperatures (10, 15, 20, 25 and 30°C) and light intensities (1, 5, 10, 20, 40, and 80 μmol m-2 s-1), the optimal infiltration conditions of the evaluated three Pyropia species were 20-25°C and 5-80 μmol m-2 s-1 for P. yezoensis, 20-30°C and 20-80 μmol m-2 s-1 for P. seriata, and 20-25°C and 20-80 μmol m-2 s-1 for P. dentata. The infiltration efficiency of free-living conchocelis for different shell types was greater in Korean and Chinese oyster Crassostrea gigas shells than that in scallop Argopecten irradians and clam Meretrix lusoria shells. These results suggest that oyster shells are suitable substrates for shell-living conchocelis production. In conclusion, the present results for optimal infiltration conditions for free-living conchocelis of the three examined Pyropia species will contribute significantly to the production of stable shell-living conchocelis.
The Korean Journal of Mycology, 2017
Twenty-two yeast strains of 15 species were isolated from the leaves of 20 trees on Oknyeobong Peak, and 24 yeast strains of 12 species were isolated from the leaves of 20 trees on Yeonjasan Mountain in Daejeon city, Korea. Cryptococcus bestiolae (5 strains) and Aureobasidium pullulans (8 strains) were the predominant isolates from Oknyeobong Peak and Yeonjasan Mountain, respectively. Of a total of 46 yeast strains, Cryptococcus kuetzingii JSL508, Cryptococcus vishniacii JSL509, and Dioszegia takashimae JSL510 from Okyeobong Peak, and Plowrightia periclymeni JSL514, Erythrobasidium hasegawanium JSL0193, and Rhodotorula nothofagi JSL0196 from Yeonjasan Mountain were determined to be yeast strains that were yet unrecorded in Korea. Morphological and cultural characteristics of these unrecorded yeasts were investigated. Erythrobasidium hasegawanium JSL0193 and Rhodotorula nothofagi JSL0196 did not form ascospores and pseudomycelia. All the strains, except Dioszegia takashimae JSL510, were halotolerant or halophilic, and Cryptococcus kuetzingii JSL508 and Dioszegia takashimae JSL510 were thermophilic, growing at 37°C.
Korean Journal of Soil Science and Fertilizer, 2018
Long-term monitoring of soil chemical properties is the main agricultural practices to improve crop yield and soil fertility. Field monitoring was performed to evaluate the chemical properties of 200 greenhouse soil samples every 4 years from 2000 to 2016 in Gyeongnam province, South Korea. Soil properties such as pH, electrical conductivity (EC), amount of organic matter (OM), available phosphate (P 2 O 5), nitrate nitrogen (NO 3-N), exchangeable potassium (K), calcium (Ca), magnesium (Mg), and sodium (Na) were analyzed. In 2016, the average concentration of soils under greenhouse condition showed 6.8 for pH, 3.52 dS m-1 for EC, 40 g kg-1 for OM, 1,065 mg kg-1 for P 2 O 5 , 154 mg kg-1 for NO 3-N, 2.35 cmol c kg-1 for K, 12.8 cmol c kg-1 for Ca, 4.0 cmol c kg-1 for Mg, and 0.91 cmol c kg-1 for Na. In addition, the average concentrations of OM, K, Ca, and Na have tended to increase with year. In 2016, the frequency distribution of excessive level of soils under greenhouse condition was 43% for pH, 61% for OM, 94% for P 2 O 5 , 89% for K, 96% for Ca, and 83% for Mg. Soil EC values of green pepper, tomato, and lettuce were significantly higher than those of carrot, strawberry, and squash (p < 0.05), whereas soil pH was significantly lower in the carrot and lettuce than that in the strawberry (p < 0.05). Soil pH was significantly correlated with the EC, NO 3-N, K, Ca, Mg, and Na. The value of EC was also positively correlated with the OM, P 2 O 5 , NO 3-N, K, Ca, Mg, and Na. In conclusion, the long-term information on soil chemical properties will be helpful to improve sustainable soil and nutrient management for greenhouse farming.
The Korean Journal of Helicobacter and Upper Gastrointestinal Research, 2015
Background/Aims: Helicobacter pylori eradication rates achieved by first-line triple treatment with proton pump inhibitor (PPI), clarithromycin, and amoxicillin have decreased to 70∼85%, in part due to increasing antimicrobial resistance. This study evaluated the trend in H. pylori eradication rates during the most recent 8 years in Daegu, Korea and analyzed different clinical factors between success group and failure group of H. pylori eradication. Materials and Methods: This was a retrospective study at a single institution. A total of 768 H. pylori-positive patients who received one or two weeks of first-line triple regimens were included between January 2007 and October 2014. Results: The overall H. pylori eradication rate was 86%. The eradication rate from years 2007 to 2014 was 80.5%, 89.4%, 95.6%, 85.5%, 87.9%, 75.8%, 83.3%, and 85.8%, respectively (P=0.027). There was no significant difference in the eradication rate among various PPIs (P=0.358). In addition, there were no significant difference of clinical factors between success and failure group of H. pylori eradication. Conclusions: The eradication rates of first-line triple therapy for H. pylori over 8 years were 75.8∼95.6%. No significant difference in clinical factors were noted between success and failure group of H. pylori eradication. Triple therapy may be a useful regimen for
Current Status of EM (Effective Microorganisms) Utilization
KSBB Journal, 2011
- Effective Microorganisms (EM), a fermented medium developed by Professor Higa at the University of the Ryukyus, is a mixed culture containing dozens of microorganisms which are beneficial to nature including people, animals, plants and many microbial species in environment. EM is known to contain more than 80 kinds of anaerobic or aerobic microbes including photosynthetic bacteria, lactic acid bacteria, yeast, actinomycetes, fungi and so on, with yeast, lactic acid bacteria and photosynthetic bacteria as the main species of EM. Antioxidant effect generated by the concert of complex coexistence and coprosperity among these microbes is considered to be the main source of EM benefits. Currently, EM is earning an increasing attention with applications in agriculture, forestry, animal husbandry, fisheries, environment and medicine among others. At the same time, however, a quantitative interpretation of EM system based on a mixed culture model needs efforts from biochemical engineers for efficient production and further promotion of EM. In this paper, we describe the functions of major microbes in EM and current researches and applications of EM in agriculture, forestry, animal husbandry, fisheries, environment and medicine.
Korean Journal of Fisheries and Aquatic Sciences, 2020
No established method can be used to select effective antibiotics in antibiotic susceptibility tests for fish bacterial pathogens quickly and accurately. Here, we established the optimal conditions for determining the minimal inhibitory concentration (MIC) of major fish bacterial pathogens (Streptococcus spp., Edwardsiella tarda, Vibrio spp., Aeromonas spp., and Pseudomonas spp.) using the KRAQ1 and CAMPY2 panels. The MIC panel used 18 antibiotics of two types and we conducted experiments to establish the optimal culture medium and temperature for each species. The optimal conditions for incubating Streptococcus spp. were in cation-adjusted Mueller-Hinton broth with TES buffer (CAMHBT) at 28°C, using 5% lysed horse blood (LHB) as recommended by the Clinical Laboratory Standards Institute. For Vibrio spp., the optimal culture conditions were 28°C in CAMHBT supplemented with 1% NaCl. The optimal conditions for culturing E. tarda, Aeromonas spp., and Pseudomonas spp. were in CAMHBT at 28°C.
Report on the External Quality Assessment Scheme of Hepatitis Viral Markers in Korea, (2016-2017)
Journal of Laboratory Medicine and Quality Assurance, 2018
As part of the immunoserology program of the Korean Association of External Quality Assessment Service, we organized two trials on the external quality assessment of hepatitis viral markers in 2016 and 2017. The hepatitis viral antigens and antibodies program consisted of 10 test items. We delivered two and three types of pooled sera specimens to 965 and 965 institutions for the first and second trials of external proficiency testing in 2016, respectively. The number of participating laboratories was 915 (94.8%) and 913 (95.0%) in the first and second trials in 2016, respectively. We also delivered three kinds of pooled sera specimens to 936 and 1,015 institutions for the first and second trials of external proficiency testing in 2017, respectively. The number of participating laboratories was 920 (98.3%) and 996 (98.1%) in the first and second trials in 2017, respectively. The most commonly tested items were hepatitis B surface antigen, followed by the antibodies to hepatitis B surface antigen, anti-hepatitis C virus, hepatitis B envelope antigen, antibodies to hepatitis B envelope antigen, anti-hepatitis A virus and antibodies to hepatitis B core antigen. The most frequently used methods for detecting viral markers were the chemiluminescence immunoassay and the electrochemiluminescence immunoassay, but they yielded a few-false positive results due to the matrix effect. The immunochromatographic assay yielded falsenegative results for anti-hepatitis A virus due to low sensitivity. Continuous improvement in the quality of viral hepatitis testing through participation in the survey seems necessary.
Isolation and screening of pyocyanin producing Pseudomonas spp. from soil
International Journal of Advanced Research in Biological Sciences (IJARBS), 2017
In 2017, as a study to discover indigenous prokaryotic species in Korea, a total of 6 bacterial strains assigned to the genus Pseudomonas were isolated from soil. From the high 16S rRNA gene sequence similarity (≥ 99.5%) and phylogenetic analysis with closely related species, the isolated strains were identified as independent Pseudomonas species which were unrecorded in Korea. The six Pseudomonas species were Pseudomonas mandelii, P. canadensis, P. thivervalensis, P. jessenii, P. lurida, and P. brenneri. Gram reaction, culture conditions, colony and cell morphology, basic physiological and biochemical characteristics are described in the species description section.