Comparison Of Antibiotic Resistance In Bacterial Flora Of Shrimp Farming Systems (original) (raw)
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A study was accomplished in regard to the microbiological abundance in the muscle of Indian White Shrimp (Penaeus indicus) collected from three departmental chain shops and three local markets of Dhaka metropolitan city has been assessed in terms of total bacterial count (TBC), coliform and Salmonella-Shigella (SS) counts. The TBC ranged from 2.1 ± 0.20 × 104 to 4.7 ± 0.50 × 105 CFU/g for departmental shop frozen shrimp and 4.2 ± 0.45 × 106 to 1.3 ± 0.50 × 108 CFU/g for local market iced shrimp. The total coliform count ranged between 1.6 ± 0.20 × 102 and 2.1 ± 0.25 × 103 CFU/g for the chain shop frozen shrimp and 2.8 ± 0.30 × 103 and 7.8 ± 0.50 × 105 CFU/g for the retail market iced shrimp. Furthermore, the Salmonella-Shigella (SS) count for chain shop frozen and local market iced shrimp varied from 0.5 ± 0.0 × 102 to 1.3 ± 0.10 × 102 CFU/g and 0.7 ± 0.0 × 102 to 2.1 ± 0.25 × 102 CFU/g, respectively. Presence of Vibrio spp. was confirmed in 11 samples (5 from departmental shops and 6 from local markets). In case of antibiotic sensitivity pattern of the indicator and pathogenic isolates, all of them were resistant to penicillin and bacitracin. Most of the isolates were sensitive to streptomycin, chloramphenicol and kanamycin. The results confirmed that the samples of local markets contained high pathogenic bacterial load which are supposed to be threat to food safety creating food borne diseases.
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Microbial community inhabiting the intestine of the shrimp (Penaeus monodon) and their surrounding environments (e.g., water and sediment) is considered as a key contributing factor for the sustainable farming of shrimp. Indiscriminate application of antibiotics in aquaculture is a growing concern due to the emergence of antibiotic resistant bacteria (ARB), more specifically the antibiotic resistance genes (ARGs). The present study investigates the microbiome composition and 19 ARGs from four different shrimp farming systems; (i) cluster, (ii) extensive, (iii) semi-intensive, and (iv) improved extensive in the southwest coastal region of Bangladesh. In doing so, the study applied advanced 16S rRNA-based metagenomic sequencing to study the bacterial composition. Moreover, gene specific polymerase chain reaction (PCR) was employed to detect the ARGs in shrimp, water, and sediments of different farming systems. In the current study, bacteria from the phylum Proteobacteria and Firmicute...
Global Journal of …, 2012
The shrimp sector is the second largest export earner for Bangladesh, which is a major contributor in the national economy of Bangladesh. It also provides millions of employment. Every year a huge amount of shrimps are rejected to export due to its pathogenic bacterial contamination. A comparative study regarding prevalence and antimicrobial susceptibility of pathogenic bacteria in the black tiger shrimp (Penaeus monodon Fabricius) were analyzed in terms of coliform, fecal coliform, Salmonella-Shigella and Vibrio spp. Total coliform, total faecal coliform, total Salmonella-Shigella and total vibrio counts were ranged from 6×10 to 8.6×10 cfu/gm, 2 5 0.0 to 3.8×10 cfu/gm, 0.0 to 3.7×10 cfu/gm and 0.0 to 2.5×10 cfu/gm, respectively. Among 11 tested isolates 5 5 3 selected from shrimp, 7 isolates displayed multidrug resistance to more than three antibiotics. Inadequate fish storage facilities, limited use of ice to preserve the shrimp, improper and unhygienic handling of shrimp could be considered as some of the factors contributing to the occurrence of these pathogens. The main reason of antibiotic resistance could be the contamination of shrimps with the antibiotic resistant bacteria through the environment and human handling.
The present study was carried out on microbial investigation to screening of pathogenic microorganism of selected commercially important shrimp samples Parapenaeopsis stylifera (Milne-Edwards, 1837); Penaeus merguiensis (de Man, 1888) Penaeus japonicas (Spence Bate, 1888) Ganjampenaeopsis uncta (Alcock, 1905) and Penaeus indicus (Milne Edwards, 1837) collected from fishing harbour of Visakhapatnam, east coast of India. The study was performed during the period of April 2015 to March 2016. Selectively, five commercially important shrimp were used to evaluate the quality of samples in terms of microbial content, using Agar plate method, Total Bacterial Count (TBC), Total coliform counts (TCC) was performed. The TBC ranged from 1.82X10 2 cfu/g to 3.23X10 2 cfu/g and TCC was found in between 2.14X10 2 and 5.22X10 2 whereas the total coliform count was not exceeded the acceptable limit recommended by FAO. The highly pathogenic bacteria Salmonella sp., Vibrio sp., was found in the collected shrimp samples.
Antibiotic resistance of bacteria from shrimp ponds
Aquaculture, 2001
The incidence of antibiotic resistance was compared in bacteria isolated from pond water, pond Ž sediment, water and sediment from the receiving environment area where water from pond . drains, which is 0 and 50 m away from the exit gate, in this study and cultured shrimp from ponds that have not used any antimicrobials, ponds that have previously used antimicrobials and ponds that are currently using oxolinic acid. Most of the bacteria isolated from all sample and pond type were Vibrios. Among the Vibrios, V. harÕeyi were most commonly isolated. Multiple Ž . antibiotic resistance MAR to at least two antimicrobials was highest in ponds currently using Ž . oxolinic acid 24% of bacteria isolated from such ponds , followed by those that have previously Ž . used antimicrobials 19% and the least was those from ponds that have not used any antimicro-Ž . bials 17% . The lowest incidence of antibiotic resistance was observed in ponds that have not Ž . used any antimicrobials 41% of the isolates from such ponds . Among the individual antibiotics, Ž . incidence of resistance to oxytetracycline was highest 4.3% of the total number of isolates Ž . Ž . Ž . followed by furazolidone 1.6% , oxolinic acid 1% and chloramphenicol 0.66% .