Bacillus subtilis NRRL B-30408: A potential inoculant for crops grown under rainfed conditions in the mountains. Journal of Soil Science and Plant Nutrition 12 (4), 811-824 (original) (raw)
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On-farm inoculation trials have been conducted to evaluate the potential of Bacillus subtilis NRRL B-30408 in respect of plant growth promotion, for two consecutive years, under rainfed conditions at a mountain location in the Indian Himalayan Region (IHR). For this, one cereal (Oryza sativa) and two millets (Eleusine coracana and Echinochloa frumentacea), were used as test crops. Observations were recorded in terms of plant growth, yield, chlorophyll content, and root colonization by various groups of microorganisms. Positive influence of bacterial inoculation on all the three crops, statistically significant in case of most parameters examined as compared to untreated controls, was recorded in both the years. There was a significant increase in chlorophyll concentration in leaves of inoculated plants. While the bacterial inoculation stimulated the free living bacterial and actinomycetes populations in the rhizosphere, the fungal population was somewhat suppressed; indicative of the antifungal property of the inoculant. The treatment also enhanced the colonization of arbuscular mycorrhizae (AM), and the endophytes associated with the roots. In conclusion, the plant growth promotion, recorded as a result of microbial inoculation, is attributed to the microbial shift in the native microflora of the rhizosphere. Based on the results of this field study, B. subtilis NRRL B-30408 can be proposed as an efficient bioinoculant for the hill crops, grown under rainfed conditions of the mountain ecosystem. The main aim of this study was on farm demonstration of the microbe based technology with peoples' participation, with a view of adoption of the technology by the farmers.
Field-based experiments were conducted to evaluate the promotion abilities of Bacillus subtilis NRRL B-30408 for growth of lentil (Lens esculenta Moench) at a mountain location of Indian Himalaya in two consecutive years. Observations were recorded for plant growth, yield, nodulation, root colonization by arbuscular mycorrhizal and endophytic fungi, and other related parameters. A positive influence of bacterial inoculation on plant biomass and yield-related parameters was recorded in both years. The significant increase in growth and nodule numbers as well as leghaemoglobin and protein concentrations of nodules indicated an enhancement in efficiency of the Rhizobium-legume symbiosis due to bacterial inoculation. An increase in protein concentration was also recorded for shoots, leaves, and seeds. Due to bacterial inoculation, there was an increase in colonization by endophytic fungi and a simultaneous decrease in colonization by arbuscular mycorrhizal fungi in roots. Based on the results of this field study, inoculation with suitable plant growth-promoting rhizobacteria instead of dual inoculation is suggested as a better option for improving the yield and related attributes of a primary dietary legume such as lentil.
2019
The effects of a microbial inoculant (Thervelics®: a mixture of cells of Bacillus subtilis C-3102 and carrier materials) on rice (Oryza sativa cv. Milkyprincess) and barley (Hordeum vulgare cv. Sachiho Golden) were evaluated in four pot experiments. In the first and second experiments, the dry matter production of rice and barley increased significantly by 10–20% with the inoculation of the mixture at a rate of 107 cfu ⋅ g–1 soil compared with the non-inoculated control. In the third experiment, the growth promoting effects of the mixture, the autoclaved mixture and the carrier materials were compared. The dry mater production of rice grains was the highest in the mixture, and it was significantly higher in the three treatments than in the control, suggesting that the carrier materials may also have a plant growth promoting effect and the living cells might have an additional stimulatory effect. To confirm the efficacy of the living cells in the mixture, only B. subtilis C-3102 cell...
Bio-efficacy of Bacillus subtilis against Damping off Disease in Brinjal
Annual Research & Review in Biology
Bacillus sp is one of the biocontrol agents are extensively used in management of fungal diseases of crop plants, exhibiting mycoparasitism against a wide range of plant pathogens. In the present investigation Efficacy of Bacillus subtilis was tested against Pythium aphanidermatum under glass house and field conditions. With regard to the germination and seedling growth parameters, the treatment T2 (liquid formulation of Bacillus subtilis @10 ml/L) recorded the highest germination percentage (92.59%), shoot length (59.3 cm), root length (24.3 cm), vigor index (7740.52) and yield (22.67 mt/ha). This was followed by the treatments T3, T1 and T4 in the decreasing order of merit. In the biometric observations also, 10 ml/L and 20 ml/L concentration of liquid formulation of Bacillus subtilis recorded statistically significant results. Observations on total protein content in brinjal plants treated with different concentrations of Bacillus subtilis revealed that treatment T2 was found be...
Microbial inoculation of soil for improving crop yield: a reality
A growing population exerts pressure on food demand, hence on food production per unit acreage. Thus, increasing crop yields has been mainly concerned with achieving food security in the way of sustained novel agricultural strategies. However, with the new tendency to accept natural products, natural fertilizers, bio-control methods and modern biotechnological approaches are being considered for this purpose while reducing the dependency on chemical methods. Since the rhizosphere is strategically placed between root and soil, much attention is given to manipulate it in order to achieve better crop production by increasing nutrition and health of plants. The mechanisms of action demonstrated by various microbes on various crops in providing these benefits to the plant is discussed. Particular emphasis is given to discuss the appropriateness of usage of the term biofilmed biofertilizer. The present status of the applicability of the rhizosphere microflora in improving crop productivity is also questioned. The modern studies on microbiomics of the rhizosphere in relation to genetics of the plant is given consideration as the means of manipulating the rhizosphere. Identifying the genes and transferring genes to different varieties of crops is suggested as the way forward.
The use of a microbiological preparation based on Bacillus subtilis in organic viticulture
BIO Web of Conferences
In organic farming, microbiological preparations are an alternative to chemical pesticides and mineral fertilizers. The article summarizes the experience of a three-year use of the biofungicide Extrasol in combination with colloidal sulfur in the vineyards of the Crimea. The use of the biofungicides makes it possible to control the development of powdery mildew of grape at the level of chemical plant protection products, including during the years of epiphytoties: on the cv. Bastardo magarachskiy grapes – 7.1–14.7 %, on the Italy grape cultivar – up to 5.2%. The degree of influence of the biofungicide Extrasol on the phenolic and oxidase complex of grapes depends on the background level of development of Uncinula necator and the biological effectiveness of the treatment. In comparison with chemical means of protection, the smallest effect of processing cv. Bastardo magarachskiy grapes with a biological product in relation to the accumulation of phenolic compounds was observed at a l...
Applied Soil Ecology, 2012
The rhizobacterial isolate RM-2 was characterized on the basis of sequencing of a partial 1270-bp fragment of 16S rDNA amplicon and validated as Bacillus sp. In view of using this strain as bioinoculant for sustainable agriculture in semi arid deserts, the plant growth promoting attributes were tested both in laboratory and field conditions. The isolate was found to have many beneficial attributes such as P solubilization, ACC deaminase activity, antifungal activity and ammonia and indole acetic acid production. The isolate positively influenced the growth and nutrient uptake of cowpea plants. Seeds coated with RM-2 showed a significant (P < 0.05) increase in seed germination, shoot and root length, fresh weight and dry weight, and leaf area. It was also able to colonize the rhizosphere of plants. Yield parameters such as number of pods and seeds and grain yield plant −1 were also enhanced significantly (P < 0.05) in comparison to control in pot and field trials in natural semi arid conditions. RM-2 strain can be considered as a good addition to consortium of beneficial microbes for growth promotion of cowpea plants.