Comparison between Conventional Decalcification and a Microwave-Assisted Method in Bone Tissue Affected with Mycetoma (original) (raw)
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This study was designed to evaluate the effect of decalcifying fluid types on bone tissue architecture and its staining properties following decalcification at varying temperatures. A decalcification methodology using Golding and Stewards (GS) fluid, and Jenkings fluid (JK), and a modern household microwave oven to accelerate the decalcification rate of bone tissue samples, was adopted for microscopic investigations at room temperature, 30 0 C, 40 0 C, and 50 0 C respectively. Bone biopsy was obtained from a rabbit's limb and fixed immediately for 24hrs in 10% formalin. The result showed that micrographs from bone tissue sections decalcified with 5% GS presented excellent histological architecture when compared with those decalcified with 5% JK at 30 0 C while 5% JK presented good histological architecture when compared with those decalcified with 5% GS at 40 0 C.Severe cytoarchitectural distortion was observed for bone tissues decalcified with 5% GS at 50 0 C, while bone tissues decalcified with 5% JK at 50 0 C, presented cyto-architectural distortions that were however, not as severe as those of 5% GS at 50 0 C. Our findings suggest therefore, that decalcification with 5% GS at 30 0 C, comparatively gives optimal histological results and preserves the tissues' best staining properties.
Journal of Pharmaceutical Research International, 2021
Background: In bony specimens, elimination of calcium is obtained by a method known as “Decalcification”. It is completed through the means of chemical agents such as acids, chelators etc that combine with ions of calcium. Decalcifying agent is used in regular conventional method where the hard tissue is placed at a room temperature (20-25°C) with modifications of the solution at orderly intervals until the final cutoff point is obtained. Usage of microwave oven for the process of decalcification is a new and fast method in contrast to the routine conventional method of decalcification. In this study, an attempt has been made to regulate and compare the conventional procedure of decalcification with decalcification done by microwave oven of hard tissue specimens by using nitric acid of 10% concentration with regards to decalcification speed, conservation of tissue architecture as well as productiveness of staining. Objectives: The study will made a comparison of Conventional and Mic...
Histopathological examination of bone lesions
Background: Bone tumors are relatively uncommon and poses difficulty byclinicoradiological diagnosis alone, when added by histopathology makes the diagnosis towards accuracy. Aims and objective: Assessment of the histopathological features of bone lesions and to correlate the bony lesions with age, sex and site of presentation. Materials and methods: AtNalanda Medical College, Patna, total of 64 scraped,incisional, and excisional biopsy specimen which were clinicoradiologically diagnosed as bone lesion were taken for study over a period of one year from august 2018 to July 2019. All clinical data was collected such as age, sex, site of involvement on a proforma after informed consent to patient. Bony tissues were put for decalcification (10% nitric acid) and soft tissue was immediately fixed into 10 % formalin, thenprocessed and embedded in paraffin blocks. Sections were stained by haematoxylin and eosin stain. Detailed microscopic study was done and findings and diagnosis were noted and compared to other studies. Results: Out of the 64 cases studied over the period of 1 year, 30 lesions were non-neoplastic (46.87%), and 34 lesions were neoplastic (53.17%), out of which 27 cases are benign (42.21%) and 7 cases are malignant (10.93%). Males (59.37%) were affected more commonly than females (40.13%), with a male: female ratio of 1.46:1. Bone lesions were more common in between the age group of 10 to 30years (67.18%). Amongst nonneoplastic lesions, Chronic osteomyelitis were the most common (16 cases, 25%), followed by Tuberculous osteomyelitis (7 cases, 10.93%).Amongst the benign neoplastic lesions, Osteochondroma was the most common (14 cases, 21.87%), Giant cell tumour was the second most common (8 cases, 12.5%).The most common malignant lesion was Osteosarcoma (3 cases, 4.68%), followed by Ewing sarcoma (2 cases, 3.12%). Most of the lesions were found in the femur (18 cases, 28.12%). Conclusion: Histopathological study enables us to understand the spectrum of bone lesion and give an idea of different bone tumors in population and different age groups and sex.
Dental research journal, 2020
Background: The aim of this study was to compare conventional and microwave-assisted decalcification of sheep bone with and without teeth and to detect any difference in tissue detail preservation, staining quality, and rate of decalcification. Materials and Methods: In this method analysis study, twenty-four specimens consisting of 12 blocks of mandibular molars with their surrounding bone and 12 blocks of mandibular osseous tissue were allocated into two microwave or routine decalcification groups using 5% nitric or formic acid as decalcifying agents. In addition to decalcification rate, a number of variables were used to assess staining quality and tissue detail preservation which were compared between the two groups using Mann-Whitney test (P < 0.05). Results: Time to complete decalcification was significantly reduced in the microwave-treated samples as compared to the conventional method, regardless of the decalcifying agent (P = 0.025). For both acids, most variables related to staining quality and tissue detail preservation were similar between the techniques (P > 0.05). Patchy staining in bone samples and tissue tears in bone + teeth specimens were more common in the routine method when using nitric acid (NAc) (P = 0.046) and formic acid (FAc) (P = 0.046), respectively. In comparing acids, the performance of FAc was slightly inferior to that of NAc, especially for specimens containing both tooth and bone. Conclusion: The use of microwave technology can accelerate decalcification of bone and teeth of sheep mandibles and at the same time preserve tissue structure and staining quality. Further studies are required to help select the best demineralizing agent, especially in specimens containing bone and teeth.
Histomorphological Spectrum of Bone Lesions at Tertiary Care Centre
Background: The spectrum of pathological bone lesions ranges from inflammatory to neoplastic conditions. Bone tumours are comparatively uncommon among wide array of lesions. The roentgenogram helps in defining exact location of lesion but becomes difficult to differentiate them. They often pose diagnostic problem as they constitute a small portion of diagnostic experience among pathologist. Objective: To study histopathological spectrum of bone lesions & correlate them with age, gender and site of occurrence. Results: All bone biopsies from January 2011 to December 2015 received at department of pathology, S.Nijalingappa Medical College, India. Total 121 cases of bone biopsies were analysed. They were decalcified & processed routinely. Out of 121 bone biopsies, 35 (28.9%) cases are non-neoplastic, 77 (63.6%) are neoplastic and 9 (7.4%) were inadequate for evaluation. The incidence of benign lesions are more than malignant with 51(66.2%) and 26(33.7%) cases respectively. Chronic osteomyelitis is the most common non-neoplastic lesion. Giant cell tumor and osteosarcoma are common benign and malignant lesions respectively. Femur is the common bone involved and metaphysis, the commonest site. The maximum numbers of cases are in the age group between 11-30 years with male preponderance. Conclusion: Though bone lesions are less common, if viewed in perspective of clinico-radiology and histopathology, correct diagnosis can be reached.
Histopathological study of bone lesions-A review of 102 cases
2016
Background: A spectrum of pathological bone lesions can be presented in any form from inflammatory to neoplastic conditions. Diagnosis of all bone lesions is made by radiological modalities like plain X-ray, CT scan, MRI and bone scintigraphy. Aim and Objectives: To study histopathological features of bone lesions and correlate them with age, site and type of lesions. Material and Methods: The study was carried out at tertiary care center from April 2013 to November 2013. A total of 102 bone lesions were analyzed. Bone biopsy was performed after detailed clinical and radiological examination. After fixation, decalcification, processing and H&E staining, histopathological diagnosis was made. Results: Out of all 102 cases, 44.11% bone lesions were found between 25-50 years with male predominance. The incidence of non neoplastic lesions was 74.5% and neoplastic lesions were 25.4%. Amongst neoplastic lesions, incidence of benign tumors was 17.64% and malignant tumors were 7.8%. The Tube...
This study evaluates the rate of bone decalcification at varying temperatures, decalcification fluid and fluid concentrations. A decalcification methodology was adopted using modern household microwave oven to accelerate the decalcification rate of rabbit compact bone sample. Bone biopsy was obtained from a rabbit limb and fixed immediately for 24hrs in 10% formalin. Traditional decalcification was carried out at room temperature (RT) with 5% Jenkins fluid (JK) and 5% Gooding and Stewart (GS) fluid as controls. Microwave oven decalcification with 5% JK and 7% JK, as well as 5% GS and 10% GS served as the tests at 300C, 400C and 500C in microwave oven. Results showed that at 300C, 5%-GS and 10%-GS as well as 5%-JK and 7%-JK presented a decalcifying time of 11hrs:30mins and 10hrs:30mins as well as 15hrs and 13hrs respectively. At 400C, a decalcifying time of 8hrs, 6hrs:30mins, 10hrs:30mins and 12hrs were recorded for the respective concentrations of decalcifying fluid. Also, at 50C, the decalcifying times were 6hrs, 5hrs, and 9hrs:30mins and 8hrs respectively. The observed differences between RT and microwave oven decalcification was significant while the rate between the two decalcifying fluid were not. Our findings further encourage the use of microwave oven for bone decalcification.
Evaluation of Decalcification Techniques for Rat Femurs Using HE and Immunohistochemical Staining
BioMed research international, 2017
Aim. In routine histopathology, decalcification is an essential step for mineralized tissues. The purpose of this study is to evaluate the effects of different decalcification solutions on the morphological and antigenicity preservation in Sprague Dawley (SD) rat femurs. Materials and Methods. Four different decalcification solutions were employed to remove the mineral substances from rat femurs, including 10% neutral buffered EDTA, 3% nitric acid, 5% nitric acid, and 8% hydrochloric acid/formic acid. Shaking and low temperature were used to process the samples. The stainings of hematoxylin-eosin (HE) and immunohistochemical (IHC) were employed to evaluate the bone morphology and antigenicity. Key Findings. Different decalcification solutions may affect the quality of morphology and the staining of paraffin-embedded sections in pathological examinations. Among four decalcifying solutions, 3% nitric acid is the best decalcifying agent for HE staining. 10% neutral buffered EDTA and 5%...
H - 2 / 94, Bengali Colony, Mahavir Enclave, Part - 1, New Delhi - 110045, India, 2016
Background: Decalcification of bone is a very important procedure in pathology especially in an oncology set up for proper staging, chemotherapy response or even in diagnosis of tumors. Objectives-To evaluate the use of tissue floatation bath (TFB) for rapid decalcification of mandibulectomy specimens in an oncology setup. Materials and Methods: Bony specimens received at pathology department were sliced using bone saw and then placed in 10% formalin at 45 0 C for 4 hours. These sections were then kept overnight in 10% nitric acid at 45 0 C in TFB for decalcification. Decalcification was checked manually next day morning and if decalcification is complete, and then the tissue transferred to tissue processor for routine tissue processing. Results: Decalcification in tissue floatation bath takes approximately 18-20 hours with better cytomorphological details as compared to routine decalcification process which takes around 7-8 days for complete decalcification. Conclusion: Tissue floatation bath is a common instrument available in all laboratories performing histopathological examination. Staining quality is comparable and time required for decalcification is improved as compared to routine decalcification technique. It can be used as an alternative to the more expensive microwave technique which requires additional instrument and space. To the best of our knowledge this is the first study using tissue floatation bath for rapid decalcification process.