In Vitro Antibacterial Activities of Root-Canal Sealers By Using Two Different Methods (original) (raw)
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Antibacterial activity of three endodontic sealers with various bases
Journal of Dental …, 2005
The antibacterial activity of three endodontic sealers, a Ca(OH)2 based sealer (apexit) a Zoe based sealer (Drofill) and a resin based sealer (AH26) was assessed on the growth of an anaerobic bacteria (peprostreptococcus spp.) using the Agar diffusion test (ADT). Materials and Method: Thirty brain heart diffusion agar plates were incubated with peptostreptococcus anaerobics. Each plate was divided into two separate areas. In one area, 0.1ml droplet of a given fresh sealer and in the other area a dry material of the same sealer were placed, such that each plate had two areas with the same sealer in two forms of fresh and dry (setform). There were ten plates for each of the three sealers. The plates were placed into anaerobic jars and incubated at 37°C. After varying periods, zone of inhibition of bacterial growth were observed, measured and compared by t and Paired T-test. Results: There was no statistically significant differences between the activity of AH26 and drofill in the fresh form(P>0.05). However there were significant differences between (the two sealers in set form) (P<0.05) Drofill was more antibacterials in the set form than AH26. All sealers were more active when fresh than ages. Conclusion: It is likely that the eugenol in the Dorifill and the formaldehyde in the AH26 are responsible for their greater antimicrobial activity.
Antibacterial Properties of Four Endodontic Sealers
Journal of Endodontics, 2008
The purpose of this study was to evaluate the antimicrobial effects of root canal sealers. The direct contact test (DCT) was used to assess the antibacterial properties of AH plus, Apexit Plus, Epiphany SE, and Roe-koSeal when in contact with Enterococcus faecalis. The materials were examined immediately after setting and 1, 2, 7, and 14 days after aging in phosphate-bufferedsaline. Statistical analysis with two-way analysis of variance, one-way analysis of variance, and Tukey multiple comparison was applied to the data. Apexit Plus had a short-term antibacterial effect of 1 day on E. faecalis, whereas Epiphany SE enhanced bacterial growth for at least 7 days. AH plus and RoekoSeal were ineffective. (J Endod 2008;34:735-738)
Comparison of antimicrobial activity of three root canal sealers
International journal of health sciences
Background Endodontic sealers with antimicrobial properties can be advantageous as they would cope better with persisting residual infection and bacteria reentering from the oral cavity. Hence we compare the antimicrobial activity of three endodontic sealers AH Plus, Apexit plus and MTA Fillapex on Enterococcus Faecalis, Staphylococcus Aureus and Candida Albicans. Material and Method : Antimicrobial activity of the materials was evaluated by the method of agar diffusion. A base layer of 10 ml of Mueller Hinton agar was poured in Petri plates (90 x 15 mm). Three wells of 6 mm of diameter (one for each material) were obtained by removing a standardized portion of the agar in equidistant points with the aid of sterilized plastic straw. The wells were immediately filled with the sealers to be evaluated. The endodontic sealers AH Plus, Apexit Plus and MTA Fillapex were prepared according to the manufacturer’s instruction. Inhibition halos formed around the wells were measured wi...
The journal of contemporary dental practice, 2016
Aims: The aim of the study was to compare the antimicrobial property of newly introduced EndoSequence BC sealer with commonly used zinc oxide-eugenol-based sealer (Zical) and epoxy resin-based sealer (MM-Seal) against Candida albicans, Lactobacillus, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Materials and methods: The agar diffusion test was done to measure the antimicrobial activity of sealers. The sealers were put in the 4 mm wells prepared in the inoculated agar plates. The agar plates were incubated at 37°C for 24 hours and the zones of inhibition that appeared was measured. Chi-square test was done to evaluate intraobserver bias for all study samples. Intergroup comparison was done for all five parameters using Pearson correlation statistical analysis. Results: EndoSequence BC sealer showed maximum mean of diameter of zones of inhibition against all the microorganisms but with no statistically significant difference with other sealers. Zical did not show any zone of inhibition against the P. aeruginosa. MM-Seal did not show any inhibitory activity against the P. aeruginosa and C. albicans. Conclusion: EndoSequence BC sealer showed antimicrobial activity against all the microorganisms and proved to be a better choice when compared with other two sealers. Clinical significance: Antimicrobial properties of endodontic sealers are important to prevent the persistent infection of the complex root canals. EndoSequence BC sealer has been proved to be a better sealer in this aspect.
Antibacterial activity of two MTA-based root canal sealers
International Endodontic Journal, 2011
Aim This laboratory study evaluated the pH and antibacterial activity of Endo CPM Sealer and MTA Fillapex by two different methods, using White MTA and Endofill as references for comparison. Methodology Antibacterial activity was evaluated against Enterococcus faecalis (ATCC 29212). The agar diffusion test (ADT) was performed to evaluate the effect before setting. The materials were placed in four equidistant wells made in ten agar plates. After incubation at 37ºC for 48 h, the inhibition zones were measured using a digital paquimeter. The direct contact test (DCT) was performed to assess the antibacterial effect after setting. Suspensions of crushed materials were prepared and mixed with E. faecalis. After different periods of time (1, 6, 15 and 60 min), the survival of bacteria was assessed by using 10-fold serial dilution and cultivated on agar plates in triplicate. Colony-forming units (CFU)/mL were calculated after incubation. pH values were also measured in triplicate. Comparison between sealers in the ADT and DCT were performed by the Kruskal-Wallis test. Results In the ADT, inhibition zones were found with MTA Fillapex and Endofill. They were similar to each other and greater than the other sealers (P<0.05). None of the tested sealers demonstrated antibacterial activity in the DCT, thus all sealers had similar bacterial counts compared to the negative control group (P>0.05). White MTA and Endo CPM Sealer suspensions had pH values greater than 11, while MTA Fillapex and Endofill had lower values. Conclusions MTA Fillapex and Endofill had an antibacterial effect against E. faecalis before setting, but none of the sealers maintained antibacterial activity after setting, despite the high pH of the MTA-based materials.
In vitro antibacterial activity of endodontic sealers
Journal of Dentistry, 2006
Objectives. To evaluate the antibacterial activity of four endodontic sealers: one epoxy resin sealer (AH Plus), two zinc oxide eugenol (ZOE)-based sealers (Endomèthasone, Pulp Canal Sealer), and one sealer containing both ZOE and orthophenilphenol (Vcanalare). Methods. A direct contact test (DCT) was performed. A 10 ml suspension of Enterococcus faecalis was placed on the test material 20 min, 24 h and 7 days after mixing. Bacteria were allowed to directly contact the sealers for 1 h at 37 8C. Bacterial growth was then spectrophotometrically measured every 30 min for 7 h, and again after 24 h as well.
Comparison of antimicrobial activity of three root canal sealers: An in vitro study
International Journal of Applied Dental Sciences, 2019
Aim and Objectives: To evaluate the antimicrobial activity of three endodontic sealers AH Plus, Apexit plus and MTA Fillapex on three types of isolated root canal microorganisms: Enterococcus Faecalis, Staphylococcus Aureus and Candida Albicans using agar diffusion test. Material and Method: Three endodontic sealers were evaluated in this study: AH Plus (Dentsply, Rio de Janeiro, RJ, Brasil), Apexit Plus (Vivadent; Schaan, Liechtenstein) and MTA Fillapex (Angelus, Londrina, PR, Brazil). The following reference strains were included in this study: Candida albicans, Enterococcus faecalis, and Staphylococcus aureus. Antimicrobial activity of the materials was evaluated by the method of agar diffusion. A base layer of 10 ml of Mueller Hinton agar was poured in Petri plates (90 x 15 mm). Inhibition halos formed around the wells were measured with the aid of a millimetric rule with 0.5 mm of accuracy. All the experiments were repeated three times in different moments. Result: In inter gro...
In Vitro Evaluation of Antimicrobial Activity of Sealers and Pastes Used in Endodontics
Journal of Endodontics, 2000
The antimicrobial activity of four root canal sealers (AH Plus, Sealapex, Ketac Endo, and Fill Canal), two calcium hydroxide pastes (Calen and Calasept), and a zinc oxide paste was evaluated. Seven bacterial strains were used, six of them standard; Micmcoccus luteus ATCC 9341, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Staphylococcus epidemidis ATCC 12228, Escherichia coli ATCC 25922, and Entemcoccus faecalis ATCC 10541. There was a wild strain of Streptococcus mutans isolated from saliva obtained in an adult dental clinic. Activity was evaluated using the agar diffusion method with Brain Heart Infusion agar and Muller Hinton medium seeded by pour plate. Calcium hydroxidebased sealers and pastes were either placed directly into 4.0 x 4.0 mm wells or by using absorbent paper points. The plates were kept at room temperature for 2 hr for diffusion. After incubation at 37°C for 24 hr, the medium was optimized with 0.05 g% l T C gel and inhibition haloes were measured. All bacterial strains were inhibited by all materials using the well method. However, when the materials were applied with absorbent paper points, Enterncoccus faecalis was not inhibited by zinc oxide, and Pseudomonas aeruginosa was not inhibited by AH Plus, Fill Canal, and the zinc oxidebased paste. We conclude that sealers and pastes presented antimicrobial activity in vitro and culture medium optimization with 0.05 g% l T C gel facilitated observation of the inhibition haloes.