Anti-Neospora caninum antibodies among dairy cattle in a rural settlement, Paraná, Brazil (original) (raw)
Related papers
Veterinary Parasitology, 2004
To determine the prevalence of Neospora caninum antibodies and associated factors, blood sera from 623 female dairy cattle from 23 farms in the north of the state of Paraná, Brazil, were analyzed by means of the indirect immunofluorescent-antibody test (IFAT ! 25). Serum samples from 134 dogs living on the same farms also were tested for N. caninum antibodies (IFAT ! 50), and the presence of dogs was associated with the prevalence of N. caninum antibodies in cattle. The overall seroprevalence in cattle was 14.3%, mainly in animals over 24 months of age. Seroprevalence in Holsteins (15.1% of 558) was greater than in mixed-breed cattle (7.7% of 65). Age (!24 months) of cattle, feeding silage and/or concentrate produced on the farm were associated; antibodies were found in 21.6% of dogs; and the presence of dogs was associated with the prevalence of N. caninum antibodies in cattle.
Prevalence of Neospora caninum antibodies in cattle from Santarém, Pará, Brazil
Research in Veterinary Science, 2008
Prevalence of anti-Neospora caninum antibodies was measured in serum samples randomly collected from dairy (40 cows from four farms) and beef cattle (120 animals from 12 farms) from the municipality of Santarém, Pará State, Brazil, calculated by using the Win Episcope 2.0 statistical program. The presence of anti-N. caninum antibodies was determined by indirect immunofluorescence-antibody test with a cut-off value of 1:100. We found that 13 farms (81.25%) showed infection rates above 10%, which indicates widespread distribution of N. caninum in the region. The frequency per animal was 19%. No difference was observed between the prevalence values in dairy and beef animals or between farms, which was probably due to the small number of dairy farms examined. The results confirm, for the first time, the presence of anti-N. caninum antibodies in cattle from Pará State and the necessity to further investigate the epidemiology of N. caninum in the Amazon region.
Arquivos do Instituto Biológico, 2012
Occurrence of anti-Neospora caninum antibodies in blood samples of 250 bovine beef cattle of the microrregion of Guarapuava, Paraná State was verified by Indirect Fluorescent Antibody Test (IFAT) (≥ 1:200) and correlated to age, sex and breed of animals. The statistical analysis was carried out through Fisher's Exact and qui-square tests (p ≤ 0.05) to associate the results of the serology with the analyzed variables. From 250 evaluated samples, 33 (13.2 %) were positive for N. caninum. The titles obtained for N. caninum were 1:200 (8), 1:400 (14) and 1:800 (11). Seropositives animals were present in 40% (10/25) of the evaluated properties. These results demonstrate wide distribution of the protozoa among the beef cattle in the region of Guarapuava, PR. Animals without defined breed showed higher rates of seropositives for N. caninum (P = 0.002). The age had positive association with the incidence of antibodies against N. caninum (P = 0.02), indicating that horizontal transmission plays an important role in the epidemiology of this coccidia.
Seroprevalence of anti-Neospora caninum antibodies in cows of North-Eastern Algeria
Veterinary World, 2019
Aim: The present cross-sectional study aimed at assessing the seroprevalence of Neospora caninum infection both at herd and within herd and at determining risk factors that are associated with its seropositivity. Materials and Methods: A total of 90 cows distributed over seven herds located in two NorthEastern Algerian provinces were blood sampled in order to be tested for the presence of antibodies against N. caninum using a commercially available enzyme-linked immunosorbent assay kit. Results: The individual seroprevalence of N. caninum was found to be 12.22%, and six of the seven herds tested had at least one seropositive cow. The logistic regression model revealed that abortion (odds ratio [OR]=29.15) and parity (OR=7.38) were positively associated with the seropositivity of animals on an individual basis. Conclusion: The study confirms the existence of N. caninum infection in cattle in NorthEastern Algeria. However, a widespread infection rate of 85.71% and its significant statistical association with previous abortion (OR=29.15) need further investigations.
Seroprevalence of Neospora caninum in dairy cattle in Bahia, Brazil
Veterinary Parasitology, 1999
Article history Among the protozoa of veterinary importance, Neospora caninum is responsible for large economic and productive losses in cattle herds, with dogs being considered the definitive hosts of the parasite. The objective of this research was to study the prevalence of anti-N. caninum antibodies and the infection risk factors in dairy cattle and dogs in the Gado Bravo municipality of the Agreste region in Paraíba State, Brazil. Blood sera from 220 cows and 20 dogs were used, altogether obtained from a total of 21 farms. For detection of the anti-N. caninum IgG antibodies, the indirect immunofluorescence reaction was performed. Sera that reacted at dilutions of ≥1:200 and 1:50 for the cows and dogs, respectively, were considered positive. The analysis of risk factors was performed with the variables most associated with N. caninum infection, obtained by epidemiological questionnaire. The prevalence of anti-N. caninum antibodies was 12.27% among the cows and 57.14% on rural properties where at least one cow was positive. Among the dogs studied, a prevalence of 25% was observed. The non-vaccination of dogs against viruses, leptospirosis, or giardiasis was considered a risk factor associated with seroprevalence for N. caninum infection (odds ratio = 9.33). Therefore, it can be concluded that dairy cattle and dogs from rural properties in the Agreste region are very likely exposed to the infection caused by N. caninum.
Detection of Anti-Neospora caninum Antibodies on Dairy Cattle Farms in Southern Italy
Veterinary Sciences, 2022
Neosporosis is recognized as one of the major causes of bovine abortion worldwide. Canids are the main definitive host for this parasite and the presence of dogs in the farm is an important factor for the Neospora caninum infection in bovines. Since, in the province of Lecce, located in the Apulia region of Southern Italy, there are no studies showing the presence of the infection in farm animals, the objective was to perform a serological evaluation for anti-N. caninum antibodiesin serum from 706 dairy cattle and 21 farm dogs located in 40 farms uniformlydistributed over the territory.The presence of N. caninum infection was confirmed in 90.0% (36/40) of the 40 farms examined. The results obtained on all serum samples by an enzyme-linked immunosorbent assay (ID Screen®Neospora caninum competition ELISA kit) for anti-N. caninum antibodies showed a seropositivity rate of 21.1% (149/706) among dairy cows, with a statistically significant higher percentage of positive subjects in the a...
Tropical Animal Health and Production, 2010
The aims of the present study were to examine the seroprevalence of neosporosis in beef herds from three southern states of Mexico and determine the association with several risk factors. A cross-sectional serological survey for Neospora caninum was carried out by sampling a total of 596 animals from 31 herds in Chiapas, Veracruz, and Yucatan States and tested using an ELISA assay (IDEXX) to detect anti-N. caninum antibodies. The overall prevalence was 11.6%, (95% CI: 0.93-0.14), however, the prevalence for Chiapas was 15% (30/200) (95% CI: 0.11-0.21), in Yucatan 11.3% (21/186) (95% CI: 0.07-0.17) and in Veracruz 8.6% (18/210) (95% CI: 0.05-0.13). Of the 596 serum samples taken, 578 were from females and 18 were bulls., Only one bull sample was found seropositive from one herd in Veracruz State.
Evaluation of a single serological screening of dairy herds for Neospora caninum antibodies
Veterinary Parasitology, 2003
Twenty-one dairy herds with a history of Neospora caninum-associated abortions were used for a longitudinal serological study. A total of 1676 animals were blood sampled 3 times and used to evaluate a single serological screening for N. caninum antibodies. The results of the first serological screening were compared with the results based on three consecutive samples, whereby two or more positive or negative test results per animal were considered to determine its serological status as positive or negative, respectively. In both test regimes 95.3% of the animals had the same interpretation, of which 33.9% were seropositive, and 61.3% seronegative. Relative sensitivity of one-time sampling compared to three consecutive samplings was 94.7%, while relative specificity was 95.6%. Relative specificity differed between herds. Predictive values positive and negative of one-time sampling were 92.4 and 97%, respectively. The agreement between one-time sampling and three consecutive samplings, kappa, was 0.90. For evaluation of discrepant results age distribution and pedigree data were used to provide clues regarding likelihood of transmission. Age clustering of seropositive animals was interpreted to indicate a point source infection. Daughter-mother relationships were used for the interpretation of congenital infections. The proportion of congenital infection decreased with increasing parity of the mother. Seropositive heifers had 80% congenitally infected offspring, while in older cows 66% of the offspring was congenitally infected, possibly due an increased immunity to transplacental infection with age. It is concluded that a single serological screening of a whole herd in connection with an analysis of age distribution and pedigree data is a rapid and valid method to : S 0 3 0 4 -4 0 1 7 ( 0 2 ) 0 0 3 2 3 -0 N. caninum.