Association between some serum oxidative stress biomarkers and lipid profile in type 2 diabetic patients in Erbil City (original) (raw)
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Oriental journal of chemistry, 2023
Diabetes, a group of metabolic disorders characterized by dysregulation of oxidative stress and elevated blood glucose levels. It has been studied with emphasis on malondialdehyde (MDA) and glutathione (GSH) levels as biomarkers of lipid peroxidation and antioxidant activity in the serum of type II Diabetes examines patients. The study involved the analysis of 105 serum samples from 75 type II diabetes patients and 30 healthy individuals. MDA and GSH levels served as measures of oxidative stress and antioxidant activity, respectively. In addition, lipid profiles were examined, which include measurements such as total cholesterol (Total C), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), and highdensity lipoprotein cholesterol (HDL-C). The results showed significantly increased MDA, total cholesterol, triglycerides and glucose levels in the diabetic group compared to controls. In contrast, GSH and HDL levels were significantly lower in diabetics. In the type 2 diabetes patient group, a correlation between glucose levels and MDA concentration was observed, while no other significant associations were found between lipid profile parameters, glucose levels and MDA or GSH levels. Studies show the complex connection between diabetes and the increase in free radicals and the corresponding decrease in antioxidant synthesis. This dynamic interaction is an important factor in the development of oxidative stress.
Bioscientia Medicina : Journal of Biomedicine and Translational Research, 2020
Hyperglycemia in Type 2 Diabetes Mellitus (T2DM) can cause individuals to experience oxidative stress conditions, accompanied by a decrease in antioxidant activity in protecting cellular components against the attack of reactive oxygen species (ROS). ROS oxidized lipid components can produce Malondialdehyde (MDA). Evaluation of MDA can be used as a marker Oxidative stress which can indirectly describe the effects of ROS because ROS compounds are reactive. This study aims to determine the analysis of MDA in T2DM subjects at the Sentinel Public Health Center in Bengkulu City in 2017.research Analytic with a case control study design in 40 samples of T2DM subjects (cases) and 40 Non-DM samples (control) were carried out at the Sentinel DM Health Center in Bengkulu City. Data collection used questionnaires, measurements of Body Mass Index, examination of Fasting Blood Glucose and plasma MDA (spectrophotometry). The results of this study there were differences in characteristics (Gender,...
2019
Introduction. The pathogenesis of type 2 diabetes mellitus (T2DM) is strongly linked to oxidative stress mainly caused by chronic hyperglycaemia. The present study investigates the association between hyperglycaemia with oxidative stress markers, antioxidants and lipid profile. Materials and methods. The case-control study involved two groups, T2DM patients (n = 83) and age and sex matched controls (n = 81). Serum levels of various molecular markers malondialdehyde (MDA), reactive oxygen species (ROS) and nitric oxide (NO), superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), vitamin C, total antioxidant capacity (TAC) and lipid parameters total cholesterol, triglycerides, low density lipoprotein (LDL) and high density lipoprotein (HDL) were measured using spectrophotometric assays. Results were analysed to compare and correlate glycaemic levels with these molecular markers. Results. T2DM patients had a higher body mass index (BMI) and body fat percentage. 2 hour blood glucose, glycated haemoglobin A 1c % (HbA 1c ), total cholesterol, triglycerides and LDL were higher in diabetics, HDL was found to be lower in diabetics than in controls. Mean levels of enzymatic and non-enzymatic antioxidants SOD, CAT, GSH, vitamin C and TAC were signifi-cantly lower while oxidative stress markers NO, ROS and MDA were higher in T2DM patients. NO showed a positive correlation (r = 0.3993, p < 0.0001) whereas TAC showed a negative correlation with glycaemia (r = -0.4796, p < 0.0001). Conclusions. Poor glycaemic control in T2DM causes elevated ROS and NO levels with increased lipid peroxidation and lowered antioxidant capacity. MDA and NO being the major risk factors could be used as a parameter along with antioxidants to assess oxidative stress in T2DM patients. (Clin Diabetol 2019; 8, 4: 215-222)
TO STUDY THE OXIDATIVE STRESS AND ANTIOXIDANTS STATUS IN DIABETES MELLITUS WITHOUT COMPLICATION
National Journal of Medical Research, 2019
Objective: To study the oxidative stress and Antioxidants status in Diabetes mellitus without complication Methods: Twenty Two patients of type II diabetes mellitus without complication and Fourteen healthy, non-diabetic, age and sex matched individuals were selected as the controls. Oxidative stress markers (NO, LPO) and antioxidant markers (GSH, SOD) measured by appropriate methods in both the groups. Results: In type II diabetes mellitus without complication Free Radicals measurement were LPO MDA (23.95±0.85 μmol/L) and NO (702.1±19.1 nmol/L) and in healthy, non-diabetic, age and sex matched individuals Free Radicals measurement were LPO MDA (17.05±2.10) and NO (527.7±16.0) with p value 0.007 and <0.001 respectively which is statistically significant. Where as In type II diabetes mellitus without complication antioxidant measurement GSH (78.3±2.77μmol/L)and SOD (891.6±52.3 U/L) and in healthy, non-diabetic, age and sex matched individuals antioxidant measurement were GSH (96.2±1.69) and SOD (1323.2 ± 105) with p value <0.001 and 0.002 respectively which is statistically significant. Conclusion: The current study suggests that the oxidative stress may have role in the pathogenesis of diabetes, as indicated by the increased levels of lipid peroxidation product malondialdehyde in patients than age and sex matched healthy controls. The decreased levels of the Glutathione peroxidase and Superoxide dismutase in the antioxidant enzyme mechanisms indicates that the anti-oxidative enzyme system gets impaired more diabetic patients.
Oxidative Stress and Antioxidant Levels in Diabetes Mellitus Patients
https://www.ijhsr.org/IJHSR\_Vol.8\_Issue.10\_Oct2018/IJHSR\_Abstract.09.html, 2018
High glucose level in diabetic mellitus can lead to free radical production causing oxidative stress. The present study has been undertaken to study the oxidative stress by measuring the antioxidant levels in diabetes mellitus patients. The level of malondialdehyde (MDA), ischemia modified albumin (IMA), ascorbic acid and vitamin E concentration in serum of normal and diabetic subjects of Chhattisgarh has been studied. A total of 125 diabetic and normal healthy subjects both male and female ranging in the age group 30-65 years were included in this study. The concentration of vitamin E, MDA, IMA and ascorbate in the serum of diabetic subjects was quantitatively determined and compared with that of normal control subjects. Student's t test was used to evaluate the significant of differences between the parameters measured. The concentration of MDA and IMA increased significantly in diabetic patients as compared to normal control subjects. The ascorbate content of diabetic subjects significantly declined as compared to that of control subjects. Also, the level of the vitamin E declined about 40-50% in diabetic subjects of both the sexes compared to control subjects. The increased level of oxidative stress biomarkers such as MDA and IMA in diabetic patients accompanied with decreased level of non enzymatic antioxidants such ascorbic acid and vitamin E as observed in the present work could cause complications in patients leading to oxidative damage to proteins, lipid and nucleic acids.
Age (years)
Diabetes mellitus (DM) is associated with hyperglycemia and oxidative stress. It is associated with various life threatening complications like nephropathy, retinopathy, atherosclerosis and coronary artery disease which adds up to the mortality among diabetics. These complications develop due to late presentation of the disease and delayed diagnosis. Oxidative stress which indicates the imbalance between reactive oxygen species (ROS) and the defensive antioxidant system is increased due to prolonged exposure to hyperglycemia. Increased oxidative damage plays an important role in the pathogenesis of diabetic complication. Malondialdehyde (MDA) a marker of lipid peroxidation, is thought to play an important role in development of atherosclerosis and other complications of DM. The study was undertaken to determine the MDA levels in the membrane of erythrocytes of diabetic patients and to study the relationship of lipid peroxidation with severity of diabetes. Fasting plasma glucose (FPG), glycated hemoglobin (HbA1c), erythrocyte malondialdehyde (MDA) levels were determined in 30 normal controls and 50 type 2 diabetic cases. The cases included 27 diabetic males and 23 diabetic females. Diabetic patients, irrespective of their gender, showed an increase in the erythrocyte MDA levels which was statistically significant. The erythrocyte MDA levels showed a significant strong positive correlation with glycated haemoglobin. Increase oxidative stress in Type 2 diabetic subjects contributes to the development of diabetic complications. Therefore, measuring the MDA level during the follow up of diabetic patients would help to early diagnosis of diabetic complication.
OXIDATIVE STRESS STATUS IN PATIENTS WITH TYPE2 DIABETES MELLITUS.
Background: Oxidative stress due to chronic hyperglycemia has been implicated in the pathogenesis of type 2 diabetes and its complications. Objectives: To estimate the levels of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and lipid peroxidation marker i.e. malondialdehyde (MDA) in type 2 DM patients with and without complications i.e., neuropathy, nephropathy and cardiovascular diseases compared to normal subjects. To access the association between oxidative stress and diabetes mellitus and development of its complication. Materials and methods: Plasma levels of SOD, CAT and MDA were estimated in 50 Control and 140 diabetic patients divided into: DWC group consisted of diabetic patients without complications, CAD group consisted of patients with coronary artery disease, DN group consisted of patients with diabetic nephropathy, DNe group consisted of patients with diabetic neuropathy. Spectrophotometry and enzyme-linked immunosorbent assay (ELISA) techniques have been applied for determination of MDA, CAT and SOD respectively. Results: comparing the levels of SOD and CAT in all groups, it was observed that the lowest concentration was in CAD, DN, DNe groups followed by DWC and control groups. Oxidative stress was found increased in CAD, DN, DNe groups as compared to DWC and control groups since, the highest concentration of MDA levels were observed in these groups. A significant inverse correlation was observed of HbA1c, insulin and MDA with SOD, CAT in all diabetic groups. And a significant direct correlation between HbA1C and MDA was also observed in all diabetic groups. Conclusion: The present study confirms susceptibility of diabetic patients to oxidative stress and that poor glycaemic control is associated with free radical-mediated lipid peroxidation. The present study arrived to the conclusion that hyperglycemia and oxidative stress present a high risk for development of diabetic complications and need early intervention.
Oxidative stress and its relation to glycemic control in patients of type 2 diabetes mellitus
International Journal of Medical Science and Public Health, 2015
Background: Diabetes mellitus (DM) is one of the most frequent chronic diseases worldwide and is characterized by absolute or relative deficiencies in insulin secretion and/or insulin action associated with chronic hyperglycemia. Objective: This study was conducted with an objective to evaluate oxidative stress in patients of DM and correlate it with glycemic control. Materials and Methods: A total of 90 subjects of both sex groups, aged between 45-60 years were enrolled for this study. Of 90 subjects, 60 were patients with type 2 diabetes and 30 were healthy without diabetes. Glycemic status was categorized as good glycemic control if glycated hemoglobin (HbA1c) <7% and poor glycemic control if HbA1c ≥7%. Serum fasting blood sugar (FBS) was measured by Glucose Oxidase-Peroxidase (GOD-POD) method. Serum malondialdehyde (MDA) was measured by the method described by Kei Satoh. HbA1c was measured by turbidimetric immunoassay. Result: Patients who were diabetic with poor glycemic control have increase in the levels of MDA and HbA1c as compared with patients who were nondiabetic (p < 0.0001). The MDA level in diabetic patients with good glycemic control was increased as compared with nondiabetic controls (p = 0.0186) whereas the differences of HbA1c level between diabetic with good glycemic control and nondiabetic control were statistically insignificant (p = 0.3297). The HbA1c and MDA levels in diabetic patients with poor glycemic control were increased compared with diabetic patients with good glycemic control (p < 0.0001). In all the studied groups, MDA was positively correlated with HbA1c. Conclusion: The study suggests that MDA and antioxidants should be measured along with routine parameters of disease and antioxidants should be incorporated in medication given to diabetic patients so that it can counterbalance the oxidative stress produced during diabetes.
Effect of Oxidative Stress on Lipid Profile in Type 2 Diabetes Mellitus
International Journal of Medical and Biomedical Studies
Background: Diabetes mellitus is a group of metabolic diseases characterized by hyperglycemia resulting from defects in insulin secretion and insulin action or both. The chronic hyperglycemia is associated with long-term damage, dysfunction, and failure of normal functioning of various organs. Hyperglycaemia generates oxidative stress and progressive inflammation due to organ damage. In this study, we have analysed the serum levels of some oxidative and inflammatory markers and Lipid profile in newly diagnosed type 2 diabetes patients. Methods: Case-control study comprising of aged-sex matched subjects: newly diagnosed T2DM cases (n=30) and controls (n=30). The serum samples of subjects were analysed for levels of CRP by turbidimetry, MDA by Buege and Aust method, while NO levels by Cortas and Wakid’s kinetic cadmium reduction method using spectrophotometer. Lipid profile was analysed using agarose gel electrophoresis. Statistical analysis was done using Mini-tab 17 software with 9...
Aims and Objectives: Diabetes mellitus (DM) is often termed as a disease of premature aging. Several studies have indicated lopsided redox balance due to pro oxidant environment as one of the important etiological factors. Some recent researches also indicate a causal relationship with oxidative stress (OS). So far, no study has been undertaken on this aspect in Nepali populations. We, therefore, aimed this maiden study in Nepali population to examine redox balance by measuring OS and antioxidant status along with lipid profile in 37 patients of DM type-2 and 30 matched normal subjects. Methodology: Thirty seven patients of DM type-2 without any complications (mean age= 57.6± 10.6 years) and 30 normal subjects (mean age= 55.8 ± 14.8 years) were included in this study. Body Mass Index (BMI) and Waist/Hip (W/H) ratio were measured. Fasting blood sample was collected for the analysis of total antioxidant activity (TAA), plasma and urinary thiobarbituric acid reactive substances (TBARS) and lipid profile by standard procedures in both the groups. The statistical analysis was done with SPSS 10 version. Results: Total cholesterol, triglyceride, VLDL-cholesterol, LDL-cholesterol, plasma and urinary TBARS were significantly raised whereas, plasma TAA was significantly reduced in DM type-2 patients as compared to controls. The comparison of old and fresh cases revealed that though TAA was lower and PTBARS and UTBARS were higher in patients but did not attain the level of significance. W/H ratio is significantly higher in patients compared to normal subjects. But, no significant correlation of BMI and W/H with lipid profile is observed in both control and patients. Conclusion: Oxidative stress is raised in type 2 DM patients. This along with deranged lipid profile and decreased antioxidant status could be the risk factors in the development of complications associated with DM.