Effects of nicotine on an in vitro reconstituted model oral mucosa in terms of cytokine production (original) (raw)
Journal of Ayub Medical College, Abbottabad : JAMC
The extensive use of tobacco and its associated problematic health issues have been a concern to mankind. The World Health Organization (WHO) estimates that approximately one-third of the global population aged 15 years or older are smokers and each smoker consumes an average of 15 cigarettes daily. The objective of this study was to establish the effect of nicotine on an in vitro reconstituted oral mucosa model, the effect of treatment with this compound was measured in terms of cytokine production. Observational laboratory based study design was used to carry out the experiment. The reconstituted human epithelium model used in the study was prepared and supplied by Skin Ethic Laboratories, Nice, France. The effect of nicotine on epithelial cytokine production was assessed using commercially available assay kits (R&D systems). This was done using the enzyme linked immuno-sorbent assay. In this study there was evidence that after 5 minutes treatment on un-inflamed mucosa with nicoti...
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