Assessing Anti-malaria Effect of Naja Naja Oxiana Snake Venom by Real-time Polymerase Chain Reaction Method (original) (raw)
Related papers
Expression, Purfication and Endotoxin Removal of Brucella Melitensis Dnak and OMP31 Proteins
ISMJ, 2014
Background: New strategies are needed to protect against Brucella melitensis infection. Subunit vaccines offer a promising approach because they can stimulate both cellular and humoral immunity, so high production of recombinant protein with less content of endotoxin is desired. In present study, we described a method for expression and purification of B.melitensis recombinant DnaK(rDnaK) and Omp31(rOmp31) proteins while less content of endotoxins were detected in final product. Material and Methods: Recombinant pET-dnak and pDEST-omp31 plasmids were transformed into competent expression host E.coli BL21 (DE3). After induction by IPTG, bacteria were grown at 20 • C for 22h. Then recombinant proteins were purified by Ni-NTA Agarose. Purification was done while two methods using Triton X-114 in washing steps and standard protocol (without detergent) were used in parallel. Results: rDnak and rOmp31 were purified by using Urea. We could obtain 20 and 8 mg recombinant proteins from rDnak and rOmp31 from 1 liter medium, respectively. The amount of endotoxins in final products was less content of 0.05 EU/mg. Furthermore, recovery of protein was up to 80% as compared to the standard protocol. Conclusion: The method used in this study, gives a product with very low extent of endotoxin, but 20% of recombinant proteins were lost. So we think the method described here can be used for purification and endotoxin removal of other recombinant proteins with similar physiologic properties.
The Antiamoebic Effect of Oliveria Decumbens and Peganum Harmala Extract on Acanthamoeba
Arak Medical University Journal, 2022
Background and Aim Acanthamoeba is one of free-living amoebas, which are very abundant in nature. As a free-living amoeba, this parasite has a very high lethality, especially in people with underlying diseases, so researchers are always looking for a way to combat it. Drug plants are a good way to fight Acanthamoeba species. In this study, we aimed to investigate the lethal effect of the extract of Oliveria decumbens vent and Peganum harmala alcoholic extract on Acanthamoeba. Methods & Materials In this study using the extract from an extract of Oliveria decumbens vent and Peganum harmala with concentrations of 1.25, 2.5, 5, 10, and 20 mg/ml to investigate the lethal effect of this extract. The plant was treated with Acanthamoeba amoebae after three times (24, 48, and 72) hours. Ethical Considerations This study was approved by the Ethics Committee of the Jahrom University of Medical Sciences (Code: IR.JUMS.REC.1398.029). Results The present research showed that using different concentrations at three times (24, 48, and 72) hours the effect of the extract on trophozoites and cysts of Acanthamoeba was shown. The highest lethality is related to the concentration of 20 mg/ml using a combination of both extracts at the time 72 hours and the lowest lethality is related to the concentration of 1.25 mg/ml of Oliveria decumbens vent at the time 24 hours. Conclusion Observations indicate that the alcoholic extract of Oliveria decumbens vent and Peganum harmala had a perfect effect both separately and in a combination of both extracts. These two extracts had a synergistic effect on the lethal effect of Acanthamoeba amoeba.
Journal of Entomological Society of Iran, 2020
The cotton bollworm, Helicoverpa armigera (Hubner),is a wide host range pest that causes severe economic damages to agricultural crops in Iran and all around the world. During recent years, chemical insecticides have been used as the most effective strategy in control of this pest, but due to their hazardous effects, most of the researches are being conducted to offer an alternative approach for chemical control. In this regard, digestive systems, in particular inhibition of insect digestive enzymes, are considered as a target for pest control. Here, we used the original pro-region of H. armigera chymotrypsin as a potent and specific inhibitor of the pest enzyme. The structural model of the insect chymotrypsin was predicted based on homology modeling and the crystal structure of Bos taurus L. as template. The reliability of the model was assessed using VERIFY_3D, ERRAT, PROCHECK, WHAT-IF and Z-scores, and the results confirmed that the predicted structural model has an appropriate q...
Nova Biologica Reperta, 2021
Coccidian (Apicomplexa: Coccidia) parasites have been reported in almost all groups of vertebrates. These parasites may cause serious ecological and pathological effects to their hosts. Reptiles were found the be the host of the parasites of the genera Schellackia and Lankesterella, as these parasites undergo their entire life cycle in the reptilian body, with an intermediate stage of dormancy in the tissues of a hematophagus transmitter (generally a mite or a mosquito), until the next reptilian host swallows the infected insect. However, very little is known about the prevalence and intensity of these parasites in most of their distribution area, which is the focus of the current study. To do so, the prevalence and intensity of these blood parasites in three species of lizards, including Ophisops elegans, Trapelus lessonae and Eremias monticola from Markazi Province of Iran were investigated. Samples of blood were collected from the caudal vein of 51 lizards of the aforementioned s...
Geographical distribution of Malaria in Jahrom, South of Iran(2006 – 2016)
Journal of Jahrom University of Medical Sciences, 2019
Introduction: Public health issues and spread of diseases have a direct relationship with the regional geography. The present study was designed and conducted to determine spatial distribution of the incidence of malaria in the city of Jahrom, using Geographical Information System (GIS). Materials and Methods: In the present descriptive study, the geographical longitude and latitude of each patient's place of residence was determined using Moran's spatial self-correlation method, and the disease incidence spots and risk using Kringing's interpolation technique and Jeefoo et al. classification in GIS-10.3. Results: A total of 136 cases occurred over the study years, with the highest Annual Parasitic Incidence (API) in 2008, 2006 and 2007 (0.116, 0.115, and 0.108 per population of 1000 respectively) and since 2008, API has had a slightly fluctuating descending trend. The spatial analysis, Kringing interpolation and Jeefoo classification showed that Jahrom's suburbs have had a very high risk of incidence (0.275 per population of 1000), and Moran's spatial self-correlation showed that the disease was randomly distributed (Moran Index=0.04) (P=0.73) (Z-score=0.33). Conclusion: The incidence of malaria was found to be higher in Jahrom's suburbs than other regions, and spatial analysis showed that high risk regions can provide a useful malaria prevention and control planning tool.
2017
Abatract In this study investigated effect of iron nanoparticles and encapsulated Lactobacillus plantarum KC426951 on growth and survival of rainbow trout (Oncorhynchus mykiss) as separate and combined. After biometry, fish introduced to tanks)30 fish per 500 l tanks) randomly with initial weight of 12.94±0.35 g and fed with different diets for 8 weeks containing 3 dosages of Fe 3 O 4 nanoparticles (30, 60 and 90 mg.kg food-1) and two dosage of encapsulated Lactobacillus plantarum (10 7 and 10 8 cfu.g-1) as separate (5 treatments) and combined (6 treatments) and control diet without any supplement. There were 12 treatments and had three replications for each other's (36 tanks). Results showed that the highest weight gain enhanced in 60 mg/kg iron nanoparticles (T4), 10 7 cfu/g Lactobacillus plantarum (T1) and their combined (T8) and the lowest in 30 mg/kg iron nanoparticles and their combined with Lactobacillus plantarum (T6 and T7) (p<0.05). The minimum FCR and maximum SGR was observed in T1 (p<0.05). Also survival rate in T6 and T7 were lowest respectively (p<0.05). In totally, it is recommended to use of 60 mg/kg iron nanoparticles and 10 7 cfu/g encapsulated Lactobacillus plantarum in rainbow trout diet.
Productions of Rice Bran Protein Concentrate and Study Some of Its Functional Properties
2014
Large quantities of rice bran are generated as by-products during the milling of rice in Iran. Although the rice bran protein has high quality and usability in food industry but rice bran is used less now. For better use of rice bran protein, it is desirable to develop effective methods to separate or concentrate the protein component from this by product. In this investigation, with using of 1 N NaOH and in alkaline condition the rice bran protein concentrate was prepared by extraction of rice bran protein. Proximate composition, amino acid profile, and functional properties including water solubility, foam capacity and foam stability, emulsifying properties, water and oil absorption of rice bran protein concentrate were determined. The protein concentrate yield was 75%. The results of amino acid composition analysis showed that the lysine content, at 2.25%, and methionine content, at 2.17, of rice bran protein concentrate were lower than other amino acids. Water and oil absorption of rice bran protein concentrate were 2.01(g water/g protein) and 2.09 (g oil/g protein), respectively. Rice bran protein concentrate showed good emulsifying properties. Foam capacity was good in rice bran protein concentrate (P<0.05) but foam stability had decreased after 60 min until 60%. In conclusion, the amino acid composition and functional properties of rice bran protein concentrate was showed that this value-added product could be used as protein ingredient in livestock and aquaculture feed industry.
MEDICAL SCIENCES JOURNAL, 2020
Background: Removal of heavy metal ions from contaminated systems by agricultural and fruit and vegetable processing waste materials is an innovative and auspicious technology .The current study was designed for utilization of bio-adsorption white hard eggshell, as less expensive and much frequently available food waste materials, due to prospective metal bio-sorption capacity to toxic heavy metal ions, including cadmium (Cd) and lead (Pb) from Oryza sativa in Iran. Materials and methods: The effect of soaking rinsed rice samples by NaCl 2% and modified white eggshell adsorbent by different concentration as adsorbent, pH, contact time and association of cooking methods on cadmium and lead contents were studied. Heavy metal contents in raw, rinsed, soaked by adsorbent and cooked and drained rice samples were determined by flame atomic absorption spectrophotometer in 925 samples in 4 different state of raw, soaking, cooked and drained. Results: Cooking rice by soaking rinsed rice samples by NaCl 2% and white eggshell for 1 hour had the greatest effect with regards to removal of Pb and Cd levels in cooked rice. It significantly reduced the cadmium content by 90.35% and lead content by 91.21% from the cooked rice, when soaked by white eggshell for one hour contact time. Conclusion: The results of the current study suggest that eggshell waste adsorbent can be used beneficially in treating rice containing heavy metal ions.