Cell cycle and differentiation of Sca-1⁺ and Sca-1⁻ hematopoietic stem and progenitor cells (original) (raw)

2018

Abstract

Hematopoietic stem and progenitor cells (HSPCs) are crucial for lifelong blood cell production. We analyzed the cell cycle and cell production rate in HSPCs in murine hematopoiesis. The labeling of DNA-synthesizing cells by two thymidine analogues, optimized for <i>in-vivo</i> use, enabled determination of the cell cycle flow rate into G2-phase, the duration of S-phase and the average cell cycle time in Sca-1⁺ and Sca-1⁻ HSPCs. Determination of cells with 2n DNA content labeled in preceding S-phase was then used to establish the cell flow rates in G1-phase. Our measurements revealed a significant difference in how Sca-1⁺ and Sca-1⁻ myeloid progenitors self-renew and differentiate. Division of the Sca-1⁺ progenitors led to loss of the Sca-1 marker in about half of newly produced cells, corresponding to asymmetric cell division. Sca-1⁻ c...

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