Analysis of monolayer formation of α-mycolic acid derived from Mycobacterium bovis BCG pasteur strain by infrared reflection-absorption spectrometry with two-dimensional correlation analysis (original) (raw)

AIP Conference Proceedings, 2000

Abstract

Monolayer formation mechanism of α-mycolic acid (α-MA) isolated from Mycobacterium bovis BCG Pasteur strain was investigated by infrared reflection-absorption (IRRA) spectrometry with two-dimensional (2D) correlation analysis. The raw IRRA spectra did not characterize the precise feature of the MA monolayer. 2D correlation analysis, however, clearly revealed that the longer or the major chain of the MA stood up earlier than the shorter chain or the α-alkyl group when the monolayer was compressed, and that the upright chains were in the form of ordered conformation.

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