Isolation and characterization of Arcobacter spp. from fresh seafood and the aquatic environment (original) (raw)
Related papers
Relevant aspects of Arcobacter spp. as potential foodborne pathogen
International Journal of Food Microbiology, 2005
Arcobacter species are Gram-negative spiral-shaped organisms belonging to the family Campylobacteraceae that can grow microaerobically or aerobically. The Arcobacter organisms also have the ability to grow at 15 8C, which is a distinctive feature that differentiates Arcobacter species from Campylobacter species. Cultural detection of Arcobacter is generally performed by an enrichment step and takes 4 to 5 days. In the last few years, several studies comparing different culturebased protocols have been published. Furthermore, DNA-based assays have also been established for rapid and specific identification of Arcobacter spp. Recent evidence suggests that Arcobacter, especially A. butzleri, may be involved in human enteric diseases. Moreover, A. butzleri has also occasionally been found in cases of human extraintestinal diseases. However, up to now, little is known about the mechanisms of pathogenicity or potential virulence factors of Arcobacter spp. There is evidence that livestock animals may be a significant reservoir of Arcobacter spp. and over the last few years, the presence of these organisms in raw meat products as well as in surface and ground water has received increasing attention. In view of control measures to be used to prevent or to eliminate the hazard of Arcobacter spp. in food, several treatments have been evaluated for their effectiveness.
Scientific Reports
Arcobacter-like species are found associated with many matrices, including shellfish in marine environments. The culture media and conditions play a major role in the recovery of new Arcobacter-like species. This study was aimed to develop a culture media for isolation and enhanced growth of Arcobacter-like spp. from marine and shellfish matrices. For this purpose, 14 different Arcobacter-like spp. mostly isolated from shellfish, were grown in 24 different formulations of enrichment broths. The enrichment broths consisted of five main groups based on the organic contents (fresh oyster homogenate, lyophilized oyster either alone or in combination with other standard media), combined with artificial seawater (ASW) or 2.5% NaCl. Optical density (OD420nm) measurements after every 24 h were compared with the growth in control media (Arcobacter broth) in parallel. The mean and standard deviation were calculated for each species in each broth and statistical differences (p
Applied and environmental microbiology, 2014
Some Arcobacter species are considered emerging food-borne and waterborne pathogens, and shellfish have been suggested as one of their reservoirs. However, only a few studies have investigated the presence of Arcobacter in this kind of food. This study assesses the prevalence and diversity of Arcobacter spp. in shellfish by multiplex PCR (m-PCR) and culturing methods (under different atmospheric conditions) and evaluates the possible influence of environmental parameters (temperature, salinity, and harvesting bay). Arcobacter was detected by m-PCR and/or culturing in 61 (29.9%) of 204 shellfish samples. Of the positive samples by culturing, 41.1% were obtained under only aerobic incubation conditions, while 23.2% were obtained under only microaerobic conditions. Of 476 investigated isolates, 118 belonged to different enterobacterial repetitive intergenic consensus (ERIC)-PCR genotypes (strains) and to 11 different species. This study shows the highest diversity of Arcobacter species ever observed in samples from any origin. The most prevalent species was Arcobacter butzleri (60.2%), followed by Arcobacter molluscorum (21.2%). The prevalence of Arcobacter was significantly higher during the summer than in other seasons, being associated with an increase in water temperature. Results confirm that shellfish are a reservoir for a remarkable diversity of Arcobacter spp.
Occurrence and Antimicrobial Resistance of Arcobacter spp. Recovered from Aquatic Environments
Antibiotics, 2021
Arcobacter spp. are emerging waterborne and foodborne zoonotic pathogens responsible for gastroenteritis in humans. In this work, we evaluated the occurrence and the antimicrobial resistance profile of Arcobacter isolates recovered from different aquatic sources. Besides, we searched for Arcobacter spp. in seaweeds and the corresponding seawater samples. Bacteriological and molecular methods applied to 100 samples led to the isolation of 28 Arcobacter isolates from 27 samples. The highest prevalence was detected in rivers followed by artificial ponds, streams, well waters, and spring waters. Seaweeds contained a higher percentage of Arcobacter than the corresponding seawater samples. The isolates were identified as Arcobacter butzleri (96.4%) and Arcobacter cryaerophilus (3.6%). All the isolates showed a multi-drug resistance profile, being resistant to at least three different classes of antibiotics. Molecular analysis of genetic determinants responsible for tetracycline resistance...
The Veterinary quarterly, 2017
Arcobacter has emerged as an important food-borne zoonotic pathogen, causing sometimes serious infections in humans and animals. Newer species of Arcobacter are being incessantly emerging (presently 25 species have been identified) with novel information on the evolutionary mechanisms and genetic diversity among different Arcobacter species. These have been reported from chickens, domestic animals (cattle, pigs, sheep, horses, dogs), reptiles (lizards, snakes and chelonians), meat (poultry, pork, goat, lamb, beef, rabbit), vegetables and from humans in different countries. Arcobacters are implicated as causative agents of diarrhoea, mastitis and abortion in animals, while causing bacteraemia, endocarditis, peritonitis, gastro-enteritis and diarrhoea in humans. Three species including A. butzleri, A. cryaerophilus and A. skirrowii are predominantly associated with clinical conditions. Arcobacters are primarily transmitted through contaminated food and water sources. Identification of...
International Journal of Current Microbiology and Applied Sciences, 2019
Arcobacter genus is an emerging pathogen, with three main spp. are pathogenic (A. butzleri, A. skirrowii and A. cryaerophilus) in nature. In present study raw sewage water, chicken, mutton and fish market associated environmental samples were screened for presence of Arcobacter spp. from Bareilly city of India. Out of total 170 samples, 50 from sewage water, 40 each of meat shops (chicken, mutton, fish) associated environment such as swab samples from knife (10), chopper (10), wooden block (10) and utensils (10) from each meat shop respectively. 26/170 (15%) samples were positive for Arcobacter spp. by multiplex PCR detection and culture positive samples were 18/170 (10.58%). The 8/50 from sewage water, 8/40 from chicken shop, 4/40 mutton shop and 6/40 from fish market associated environment detected positive by m-PCR. Out of total 170 samples screened 18 were culture positive with isolation rate 6/50 (12%) from sewage water, 5/40 (12.5%) from chicken shop, 3/40 (7.5%) from mutton shop, 4/40 (10%) from fish market associated environments. Out of 18 Culture positive samples Arcobacter butzleri was predominant spp. isolated, than A. skirrowii and A. cryaerophilus and mix infection. The 18 positive strains were selected for disk diffusion, tested against 12 different types of antibiotic discs. High antibiotic resistance were seen as in cephalothin (17/18), co-trimoxazle (16/18), ampicillin (14/18), Vancomycin (14/18), tetracycline (14/18), kanamycin (12/18), Azitromycin (11/18) and other antibiotics shows greater sensitivity to Arcobacter butzleri with resistance pattern as 0/18 by nalidixic acid, Erytromycin (4/18), Ciprofloxacin (4/18), Gentamicin (5/18) and streptomycin (6/18). Multi drug resistance (MDR) was seen in this the study. Which may concerned to treatment failure in human and animal concerned to Arcobacter MDR strains. Sewage water may acts as reservoir of Arcobacter spp. as human, animal excreta are major part in and associated water. Most of the sewage water is recycled by municipal corporation. Hence water processing plant, purification methods must aware of probable public health risk related to sewage water. More cautions are recommended for personnel hygiene in processing, handling and awareness related to meat and meat products. The unnecessary use or misuse of antibiotics should be limited or avoided for public health safety.