Arene cis -Diol Dehydrogenase-Catalysed Regio- and Stereoselective Oxidation of Arene-, Cycloalkane- and Cycloalkene- cis -diols to Yield Catechols and Chiral α-Ketols (original) (raw)

Benzene cis-diol dehydrogenase and naphthalene cis-diol dehydrogenase enzymes,e xpressed in Pseudomonas putida wild-type and Escherichia coli recombinant strains,w ere used to investigate regioselectivitya nd stereoselectivity during dehydrogenationsofarene,cyclic alkane and cyclic alkene vicinal cis-diols.T he dehydrogenase-catalysedp roduction of enantiopure cis-diols, a-ketolsa nd catechols, using benzene cis-diol dehydrogenase and naphthalene cis-diol dehydrogenase,i nvolvedb oth kinetic resolution and asymmetric synthesism ethods.T he chemoenzymatic production and applications of catechol bioproducts in synthesiswere investigated.