Title: Serum and Follicular Fluid Vitamin D Levels and Outcomes of Assisted Reproductive Techniques; A Prospective Cohort Study (original) (raw)
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Vol 15, No 4, October-December 2021
Background: Based on studies on animal models, vitamin D plays an essential role in reproduction by controlling Ca and Mg levels. Despite these findings, the effects of vitamin D deficiency and supplementation on the outcome of assisted reproductive techniques (ART) remain controversial. Therefore, the aim of the present study was to assess the relationship between serum and follicular fluid 25-OH vitamin D levels on reproductive outcomes of infertile women. Materials and Methods: This prospective cohort study included 150 infertile women who underwent in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). The participants were allocated to one of the three groups according to their serum and follicular fluid 25-OH vitamin D concentrations (less than 10 ng/ml, between 10 and 30 ng/ ml and more than 30 ng/ml), and fertilization, cleavage and biochemical and clinical pregnancy rates were compared among the groups. Data was analyzed by SPSS software and using Chi-square and Spearman correlation coefficient. Results: Serum and follicular fluid vitamin D levels significantly correlated with biochemical (P=0.008), (P=0.003) and clinical pregnancy (P=0.017), (P=0.001) rates respectively. However, the quality of embryos (P=0.125), (P=0.106) and fertilization rate (P=0.082), (P=0.059) were not associated with the level of serum and follicular fluid vitamin D. Conclusion: This study found that women with higher levels of vitamin D in their serum and follicular fluid are significantly more likely to achieve pregnancy but without affecting the quality of embryo and fertility rate.
Correlation between follicular fluid 25-OH vitamin D and assisted reproductive outcomes
Iranian journal of reproductive medicine, 2015
Vitamin D in complex with its receptor by regulating gene expression, endometrium immune response and stimulation of endometrium decidualization can be involved in implantation. So, it seems that the amount of vitamin D in follicular fluids (FF) may have an association with ART success. First, we intended to investigate the possible association between levels of follicular fluids 25-OH vitamin D with assisted reproductive outcomes. Second, we examined relationship between 25-OH vitamin D levels with number and quality of oocytes. In a prospective study, 80 infertile female candidates for IVF/ICSI were enrolled. Blood samples (on the day of human chorionic gonadotropin administration) and follicular fluids were taken, and then levels of serum estradiol and follicular fluids 25-OH vitamin D were measured. Also clinical characteristics of patients (duration of infertility, causes of infertility, menstrual status), number and quality of oocytes, number of fertilized oocytes, estradiol l...
Journal of Parathyroid Disease, 2020
Introduction: There are controversies about relationship between serum and follicle vitamin D levels and in vitro fertilization (IVF) outcomes. Objectives: The goal of this study was to determine association between serum and follicular vitamin D levels and IVF outcome in the Iranian population. Patients and Methods: This cohort study was conducted in a women’s hospital. One hundred and sixty patients (mean age = 28 years ) who were candidates (by simple sampling) for IVF enrolled in the study. Levels of 25 (OH)-D in follicular fluid and serum were measured. Biochemical pregnancy was determined by detecting serum β-hCG. A detectable gestational sac was considered as a characteristic of clinical pregnancy. Results: Biochemical pregnancy was detected in 49 (30.6) and clinical pregnancy in 39 (24.4). Mean vitamin D levels of serum and follicular fluid were not significantly different between cases that had clinical pregnancy or not. In cases with serum vitamin D level less than 30, mea...
Archives of Medical Science
IntroductionFollicular and serum vitamin D are considered potential markers of the oocyte and embryos' quality and predictors of IVF outcomes.Material and methodsThis retrospective cross-sectional study correlated vitamin D in sera and follicular fluid of women with unexplained infertility mutually and with IVF outcomes. ELISA was used for measuring Vitamin D.ResultsResults show only a positive correlation between follicular and serum levels of Vitamin D (Rho=0.615, p=0.025) and between follicular levels of Vitamin D with the percentage of embryo fragmentation (Rho=0.544; p=0.036).ConclusionsResults suggests that serum and follicular fluid vitamin D measurements could be complementary tools to the routine assessment of embryos.
BMC Pregnancy and Childbirth, 2019
Background Vitamin D plays an important role in human physiology and pathology. The receptor for vitamin D regulates 0.5–5% of the human genome. Accordingly, vitamin D insufficiency has been shown to increase the risk of several diseases. In recent years, based on growing evidence, on a role of vitamin D has been also postulated in reproductive health both in animals and humans, especially in female fertility female fertility. In vitro fertilization success was shown to be higher in women with appropriate reserves of vitamin D. However a causal relation has not been demonstrated and randomized controlled trials testing the effectiveness of vitamin D supplementation in IVF are warranted. Methods This is a multicenter randomized double blinded placebo controlled study aimed at determining the benefits of vitamin D [25(OH)D] supplementation in improving clinical pregnancy rate in women undergoing IVF. Eligible women with a serum level of 25-hydroxyvitamin D [25(OH)D]
Purpose This study investigated the relationship between the vitamin D [25(OH)D] level in individual follicles and oocyte developmental competence. Methods A prospective cohort study in a private infertility center. Infertile women (N = 198) scheduled for intracytoplasmic sperm injection (ICSI) and a single embryo transfer (SET) provided serum samples and 322 follicular fluid (FF) specimens, each from a single follicle on the day of oocyte retrieval. Results FFs corresponding to successfully fertilized oocytes (following ICSI) contained significantly lower 25(OH)D level compared with those that were not fertilized (28.4 vs. 34.0 ng/ml, P = 0.001). Top quality embryos on the third day after fertilization, when compared to other available embryos, developed from oocytes collected from follicles containing significantly lower 25(OH)D levels (24.56 vs. 29.59 ng/ml, P = 0.007). Positive hCG, clinical pregnancy, and live birth rates were achieved from embryos derived from oocytes that grew in FF with significantly lower 25(OH)D levels than in follicles not associated with subsequent pregnancy. The concentration of 25(OH)D in FF in women with negative hCG was 32.23 ± 20.21 ng/ml, positive hCG 23.62 ± 6.09 ng/ml, clinical pregnancy 23.13 ± 6.09 ng/ml, and live birth 23.45 ± 6.11 ng/ml (P < 0.001). Women with serum 25(OH)D < 20 ng/ml had not only a higher fertilization rate (71 vs. 61.6%, P = 0.026) and a higher clinical pregnancy rate (48.2 vs. 25%, P = 0.001), but also higher miscarriage rate (14.5 vs. 3.8%, P = 0.013) compared with those with levels ≥ 20 ng/ml. Conclusion This study reveals that the level of 25(OH)D in FF correlates negatively with the oocytes' ability to undergo fertilization and subsequent preimplantation embryo development. Oocytes matured in FF with low 25(OH)D concentration are more likely to produce top quality embryos and are associated with higher pregnancy and delivery rates. On the other hand, low serum vitamin D concentration is associated with higher miscarriage rates. Keywords Oocyte quality. Vitamin D [25(OH)D]. Follicular fluid. ICSI (intracytoplasmic sperm injection). Single embryo transfer (SET)
Vitamin D deficiency and infertility: insights from in vitro fertilization cycles
Context: Vitamin D deficiency has been proven to affect fertility in mammals but data in human is less convincing. In particular, data on in vitro fertilisation (IVF), an attractive model to draw information on this topic, are sparse and conflicting. Objective: To investigate IVF outcome in women with deficient 25-hydroxy-vitamin D [25(OH)D] serum levels (<20 ng/ml). Design: Prospective cross-sectional study. Setting: Infertility Unit of an academic setting. Patients: The main inclusion criteria were as follows: 1) indication to IVF, 2) age 18-42 years, 3) BMI 18-25 Kg/m 2 , 4) adequate ovarian reserve according to Bologna criteria. Eligible women provided a serum sample for 25(OH)D measurement at the time of cycle preparation. Subjects were subsequently excluded if the cycle was cancelled or if the attempt was excessively delayed. Intervention: Quantitative detection of serum 25(OH)D. Main outcome measure: Clinical pregnancy rate. Results: The number of recruited women with serum 25(OH)D <20 ng/ml and ≥20 ng/ml was 154 and 181, respectively. The clinical pregnancy rates were 20% (30/154) and 31% (56/181), respectively (p=0.02); the adjusted Odds Ratio for clinical pregnancy in women with vitamin D ≥20 ng/ml was 2.15 (95%CI: 1.23-3.77). Subgroup analyses showed that the group of women with the highest serum levels (>30 ng/ml) had the highest chances of pregnancy. Conclusions: Vitamin D is an emerging factor influencing female fertility and IVF outcome. Additional studies are pressingly needed to confirm a causal relationship and to investigate the potential therapeutic benefits of vitamin D supplementation.
Do Serum Vitamin D Levels Have Any Effect on Intrauterine Insemination Success
Volume 12, Number 2, Jul-Sep 2018
Background Recent studies have shown that vitamin D has an essential role in the reproductive system. In this study, we aimed to investigate the effect of vitamin D levels in patients undergoing ovulation induction (OI), and subsequent intrauterine insemination (IUI) procedure. Materials and Methods One hundred and four infertile and one hundred and three fertile women were recruited in this cross-sectional study which was conducted in a tertiary level maternity hospital. Infertile patients were divided into pregnant and non-pregnant subgroups after treatment. Individual characteristics and 25-hydroxyvitamin D3[25 (OH) D3] levels were compared between the groups. Results The vast majority of our study population consisted of women who had vitamin D deficiency (96.6%). There was no statistically significant difference between infertile and fertile groups in terms of serum 25 (OH) D3lev- els (P=0.512). Similarly, no significant difference was observed between the pregnant and non-pregnant subgroups of infertile patients regarding 25 (OH) D3levels (P=0.267). Conclusion There is no association between female infertility and serum vitamin D levels. Vitamin D does not pre- dict pregnancy in infertile women undergoing OI with IUI. Further research which will provide a comparison between much more women who have deficient and sufficient 25 (OH) D3levels is warranted.
Serum and Follicular Fluid Vitamin D and Follicular Response among Infertile Women Undergoing ICSI
World Family Medicine Journal/Middle East Journal of Family Medicine, 2017
Background Some studies have shown the relation between vitamin D levels and the success rate of fertility in couples treated with Assisted Reproductive Technology (ART). The aim of this study is to determine Association of vitamin D level in serum and follicular fluid with follicular response in infertile women in an ART program. Correlation analysis revealed a high relationship between serum and follicular fluid vitamin D (P<0.001). Conclusion: Different concentrations of vitamin D in serum or follicles have no significant correlation with the number of ovarian follicles and mature oocytes.