Viability of Hemoglobin Concentration, Red Blood Cells, White Blood Cells and Platelets Counts in Blood Specimens Stored Overnight at Room Temperature (original) (raw)
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Stability of Selected Hematological Parameters in Stored Blood Samples
Journal of Cell Science & Therapy
1.1 Background: Apheresis platelets are a mainstay therapy in our tertiary cancer centre wherein 3000 donors are tested annually. Platelet drives to register willing donors are conducted. The complete blood counts to determine the eligibility for platelet donation is done on the first donor visit. However, it can be challenging to test these samples on that day due to reasons like manpower shortages, weekends, and a single cell counter in blood banks. National guidelines for blood banks in India do not elaborate on the ideal storage time for such blood samples. Hence we embarked on this study to determine the sample stability. Aim: The aim was to study the stability of blood samples given at first visit for registration as platelet donors. Specific hematologic parameters were studied at time intervals of 4, 48 and 72 hours. Materials and Methods: Eligible donor's venous blood sample was collected in K2-EDTA (Ethylenediaminetetraacetic acid) vacutainers for cell counts. This was tested on an automated cell counter (Humacount, Human, Germany) within 4 hours at room temperature. Then, the samples were stored at 4ºC and retested at 48 and 72 hours. Results: 969 blood samples were tested for hemoglobin, WBC (White blood cell) and Platelet count. There was no statistical difference in mean values of hemoglobin and WBC counts at three time intervals. Even though difference in mean platelet count was statistically significant (p<0.001), it did not impact on donor acceptance criteria. Conclusions: Specific hematologic parameters (Hb, WBC, platelet) were found to be stable at 4ºC for 72 hours. Hemoglobin (Hb) was the best preserved parameter followed by WBC and platelet count.
Introduction: The problem of medical errors has recently received a red alert of attention. For clinical laboratory medicine, the most frequent errors occur in the pre and post analytical phases. In general haematological parameters have been measured from EDTA anticoagulant whole blood, shortly after drawing. The time exciting between the blood drawing and the measurement of hematological parameters and the preparation, staining of blood smears for blood cell morphology examination. Material and Methods: Blood samples from healthy
The stability of sample storage for complete blood count (CBC) toward the blood cell morphology
Bali Medical Journal
Background: Peripheral blood smear examination had a pivotal role in determining diagnosis and as confirmation of the automatic hematology analyzer results. The storage process was highly influential on the morphological stability of the cell. This study aimed to assess the morphological changes in blood cells stored at certain period and temperature. Method: Sample of 30 blood specimens of healthy people with dipotassium ethylenediaminetetraacetic acid (K 2 EDTA) anticoagulants. The specimens were stored at room temperature (18-25ºC) and refrigerator temperature (2-8ºC) and were analyzed at the preliminary examination i.e., 8 hours, 16 hours, 24 hours, 48 hours, 72 hours, and 96 hours. Kappa test was used in validating the reading of PBS (Peripheral Blood Smear). The discrimination testing used were paired t-test and Kolmogorov Smirnov test with p value <0.005, which stated to be significant. Result: The changes in erythrocytes morphology stored at room temperature (18-25ºC) was that the erythrocytes crenation started to happen at 8 hours storage with the grading scale of +2 that were found in 24 (80%) samples, whereas at refrigerator temperature (2-8ºC) the grading scale was +1 and found in 13 (43.3%) samples. Spherocytes on erythrocytes began to form at room temperature (18-25ºC) at 8 hours storage with grading scale of +1 and was found in 2 (6.7%) samples, whereas at refrigerator temperatures (2-8ºC) spherocytes on erythrocytes began to form at 24 hours storage with grading scale of + 2 and was found in 3 (10%) samples. Conclusion: Peripheral blood smear (PBS) examination shall be done immediately to obtain significant results.
Effect of Storage Time and Temperature on Hematological and Biochemical Parameters of Blood
IOSR Journals , 2019
Delay in analysis of hematological and biochemical parameters of blood can occur due to referral from remote centres or due to storage, which may cause changes in blood parameters and erroneous laboratory results. We studied the changes associated with storage of blood at different temperatures and for different time-periods. The serial changes in complete hemogram, reticulocyte count and peripheral blood smear morphology, plasma sugar, urea, creatinine and electrolytes were measured. Blood from 60 patients, each collected &stored in K2-EDTA for hematological tests, sodium fluoride for plasma glucose and clotted blood for serum biochemistry. All the samples were analysed by auto-analysers. 20 samples were stored at room temperature (18-22°C), 20 samples at 4-8°C and 20 samples at 37°C. Analysis of samples was done at 0, 4, 8, 12, 24, 36, 48, 60 and 72 hours. When stored at room temperature, the RBC count, hemoglobin concentration remained relatively stable till 48 hours. Platelet, reticulocyte and absolute neutrophil counts changed significantly after 24 hours. Plasma glucose, serum electrolytes and protein varied significantly after 48 hours of storage. Urea and creatinine levels changed less significantly. Samples stored at 4-8 °C were relatively more stable. Constancy ofparameters, like Reticulocyte count and DLC was noted at this temperature. Urea, creatinine and hemoglobin levels can be measured after 48 hours of delay; but protein, glucose and electrolyte estimation may be unreliable.
Proceedings of the 1st International Conference on Health, 2019
Whole blood contains all the elements of blood such as all blood cells, plasma, and clotting factors. It is used in the treatment of massive bleeding. However whole blood has an expiration time limit. The quality of blood decreases gradually due to storage time and causes blood cell lysis, so it directly affects blood cell counts and pH. The aim of this study was to determine the quality of whole blood during 28day storage. Whole Blood from blood bags containing CPDA-1 was used as a sample. The number of erythrocytes, leukocytes, and platelets was measured by Hematology Analyzer. The results of the study showed that there was a decrease in the number of erythrocytes from 5,02 x 106cell/μL into 4,92x106cell/μL. The number of leukocytes decreased from 6,31x103cell/μL to 3,17 x 103cell/μL. Platelet count also decreased from 195x103cell /μL to 81x103cell/μL. The pH value decreased from 7.2 on to 6.9. This study concluded that there was a decrease in the number of erythrocytes, leukocyte...
Indian Journal of Pathology and Oncology, 2023
Background: Complete hemogram & peripheral blood smear are primary tests done in day to day practice for correct diagnosis & further treatment of patients. EDTA blood samples are used for this regular screening tests which shows less stability in case of delaying sampling. Hence, the final results of these tests can be affected by different storage conditions & duration. This present study provides data analyzed from EDTA blood samples stored upto 24 hour at both room temperature & 4°C refrigeration. Materials and Methods: This study includes total 150 blood samples from indoor & outdoor patient without any specific criteria which were collected randomly. These blood samples were analyzed using haematological analyzer for complete blood count & their peripheral smear after storage in both room temperature & 4°C refrigeration for 24 hrs. Result: There was significant increase in MCV & decrease in MCHC, reduced WBC count & platelet count with storage at room temperature which was prohibited by refrigeration. However, both room temperature & refrigerated storage does not affect RBC count & hemoglobin. Conclusion: Blood samples stored at room temperature for 24 hrs results in changes in haematological parameters & morphology of cells. Hence, refrigerated storage at 4°C is recommended for accurate results in case evaluation of delayed samples. Keywords: Sample stability, Storage conditions, Complete hemogram, EDTA anticoagulated blood
Effects of Storage of Blood at Room Temperature on Hematologic Parameters Measured on Sysmex XE2100
Labmedicine, 2006
Clinical laboratories equipped with modern automated analyzers are capable of processing large volumes of hematologic tests in an efficient and timely manner. These tests include complete blood count (CBC), differential leukocyte count (diff), reticulocyte count (retic), and more recently, the nucleated red blood cell count (NRBC). To ensure reliability of the results generated by the instrument, it is imperative that the specimens are collected appropriately in a suitable anticoagulant and analyzed on a properly calibrated instrument within the time frame considered appropriate or recommended by the manufacturer. Dipotassium ethylenediamine-tetraacetate (K 2-EDTA)-anticoagulated blood is the specimen of choice for all of these tests. Generally, specimens arrive in the laboratory and are analyzed within hours of collection from patients. However, it is not uncommon for laboratories to receive specimens that are 1 to 3 days old. Furthermore, it is a common practice to keep these specimens at room temperature until delivery to the laboratory and processing. Will these older specimens generate clinically reliable results when processed through the automated analyzer for any or all of the hematologic tests cited above? Manufacturers of automated analyzers and published literature usually cite that blood specimens kept at room temperature for 24 hours to 48 hours generally yield reliable results. 1-5 However, specific information concerning the suitability or unsuitability of specimens older than 1 or 2 days for various automated hematologic tests is limited, particularly in the recent literature. The only recent publication, from our own group 3 years ago, reported on changes in automated CBC and diff induced by storage of blood at room temperature. 6 In that study, the Beckman Coulter GenS was used to perform the CBC and diff. The retic count was not included in the study and the automated NRBC count was not available on that analyzer. For the past 2 years, our laboratory has been using XE-2100, an automated analyzer from Sysmex America, Mundelein, IL, for performing automated CBC, diff, retic and NRBC. The purpose of the present study was to delineate changes that occur in various parameters of all tests, including retic and NRBC, performed on Sysmex XE-2100, during storage of blood at room temperature. Material and Methods A total of 40 K 2-EDTA-anticoagulated blood specimens were selected from the routine laboratory workload over several days to represent a range of normal and abnormal values (Table 1). Among the 40 specimens, 12 were leukopenic (white cell count <4.0 x 10 3 /µL), 10 had leukocytosis (white cell count >11.0 x 10 3 /µL), 13 were thrombocytopenic (platelet count <140 x 10 3 /µL), and 6 had thrombocytosis (platelet count >400 x 10 3 /µL). All but 9 were anemic, with hemoglobin values less than 12.5 g/dL. Specimens were kept at room temperature throughout the study. Each specimen was run on 1 of the 2 Sysmex XE-2100 analyzers in our laboratory for CBC, diff, retic, and NRBC once daily for 5 days. To determine within-day precision, 25 of the 40 specimens, representing a range of normal and abnormal values (Table 2), were analyzed a second time, within 8 hours of the initial run, on either the same or a different XE-2100 analyzer. The XE-2100 utilizes impedance technology for red cell count and platelet count, light scattering technology for the white cell count, and a photocolorimetric
2020
The performance of hematological tests deteriorates with the increase in the length of sample preservation. Therefore it has been an issue to characterize the maximum permissible period spent between blood collection and measurement to have the acceptable test report. From this view point, a study was undertaken to know about the effect of preservation length on complete blood count (CBC) in rat of Long Evans strain. A total of 30 samples were collected from 10 apparently healthy rats aged between 45-48 days and the blood samples were kept in commercial test tubes treated with EDTA. The test tubes containing whole blood samples were divided into three different groups based on preservation length and were allowed to keep at 4ºC for three different lengths of time viz. 2 hours, 4 hours and 6 hours until analysis. The samples were then analyzed for their complete blood count (TEC, TLC, Hb, PCV, DLC, Absolute Leukocyte Count, Red Cell Indices, RDW-SD, RDWCV, Platelet, MPV, PCT and PDW) using Sysmex XT-1800i auto hematological analyzer. Result showed that no significant change in CBC with the variation in preservation length. Based on these findings, it can be concluded that blood samples can be preserved for as long as 6 hours to have the same report obtainable when the samples are preserved at 4ºC in refrigerated condition for 2 or 4 hours.
Sample stability for complete blood cell count using the Sysmex XN haematological analyser
Blood transfusion = Trasfusione del sangue, 2015
Sample stability is a crucial aspect for the quality of results of a haematology laboratory. This study was conducted to investigate the reliability of haematological testing using Sysmex XN in samples stored for up to 24 h at different temperatures. Haematological tests were performed on whole blood samples collected from 16 ostensibly healthy outpatients immediately after collection and 3 h, 6 h or 24 h afterwards, with triple aliquots kept at room temperature, 4 °C or 37 °C. No meaningful bias was observed after 3 h under different storage conditions, except for red blood cell distribution width (RDW) and platelet count (impedance technique, PLT-I) at 37 °C. After 6 h, meaningful bias was observed for mean corpuscular haemoglobin (MCH) and mean corpuscular volume (MCV) at room temperature, red blood cell (RBC) count, mean corpuscular haemoglobin concentration (MCHC), MCH, MCV and PLT-I at 4 °C, and RBC, RDW, MCHC, MCH and PLT-I at 37 °C. After 24 h, a meaningful bias was observed...