Escherichia coli as uropathogen: antibiotic susceptibility profiling (original) (raw)
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Iranian Journal of Public Health, 2014
Antibiotic resistance among uropathogens is emerging public health problem. This study was done for assessing antibiotic and multidrug resistance (MDR) patterns of Escherichia coli at Bir Hospital, Kathmandu, among suspected urinary tract infection (UTI) patients from January to March, 2011. Altogether, 739 urine samples were analysed by semi-quantitative culture method and uropathogens were identified by conventional methods. E. coli was tested (109 samples) for antimicrobial susceptibility by Kirby Bauer disc diffusion method as per Clinical and Laboratory Standard Institute (CLSI) guidelines. Out of 739 samples, 27.3% gave significant growth while 3.1% and 29.2% samples gave mixed and non-significant growth respectively. E. coli was found to be most predominant isolate (54.0%) followed by coagulase negative Staphylococci (CoNS) (21.3%) and Enterococcus spp. (7.3%). Nitrofurantoin was found to be the most effective antibiotic followed by ciprofloxacin and ofloxacin while cephalexin was least effective. Out of 109 E. coli isolates, 90.8% were MDR strains and most of the isolates had a very high multiple antibiotic resistance (MAR) index, suggesting the origin of the isolates to be of high antibiotic usage. E. coli showed higher rate of resistance towards commonly used oral antibiotics. However, nitrofurantoin is still active against organisms. Thus, nitrofurantoin could be the choice for empirical therapy of UTI.
Antibiotic resistance among uropathogens is emerging public health problem. This study was done for assessing antibiotic and multidrug resistance (MDR) patterns of Escherichia coli at Bir Hospital, Kathmandu, among suspected urinary tract infection (UTI) patients from January to March, 2011. Altogether, 739 urine samples were analysed by semi-quantitative culture method and uropathogens were identified by conventional methods. E. coli was tested (109 samples) for antimicrobial susceptibility by Kirby Bauer disc diffusion method as per Clinical and Laboratory Standard Institute (CLSI) guidelines. Out of 739 samples, 27.3% gave significant growth while 3.1% and 29.2% samples gave mixed and non-significant growth respectively. E. coli was found to be most predominant isolate (54.0%) followed by coagulase negative Staphylococci (CoNS) (21.3%) and Enterococcus spp. (7.3%). Nitrofurantoin was found to be the most effective antibiotic followed by ciprofloxacin and ofloxacin while cephalexin was least effective. Out of 109 E. coli isolates, 90.8% were MDR strains and most of the isolates had a very high multiple antibiotic resistance (MAR) index, suggesting the origin of the isolates to be of high antibiotic usage. E. coli showed higher rate of resistance towards commonly used oral antibiotics. However, nitrofurantoin is still active against organisms. Thus, nitrofurantoin could be the choice for empirical therapy of UTI. Key words: Escherichia coli, multidrug resistance, multiple antibiotic resistance index, urinary tract infection
International journal of health sciences
One of the most prevalent infections, urinary tract infection (UTI) can affect anyone from a newborn to an elderly person. The most common cause is a bacteria called uropathogenic Escherichia coli (UPEC). The ability to adhere to host epithelial cells in the urinary system is the most important predictor of pathogenicity, however fimbriae, pili, flagella, and secreted (toxins) virulence factors all play a role in the pathogenicity of UroPathogenic Escherichia coli strains. Effective management of UTIs requires up-to-date information on the antibiotic susceptibility pattern of uropathogens. Thirty midstream urine samples were collected from patients at Al-Ramadi Teaching Hospital's Urology Clinic. Isolates of E. coli (the most prevalent bacteria) from the cultured samples are tested for susceptibility to the most common antibiotics in use today. Thirty (75%) of the total 40 samples were culture positive; the most common organisms found were Escherichia coli (16 (53.3), Citrobacte...
Journal of the Pakistan Medical Association, 2020
The present research study was done to determine the correlation between antibiotic susceptibility and drug prescription patterns in empirical treatment of uropathogenic Escherichia coli (E. coli) in two hospitals of district Muzaffarabad, Azad Kashmir, Pakistan. One hundred uropathogenic E. coli clinical isolates were collected from UTI patients who attended the Combined Military Hospital (CMH) and Abbas Institute of Medical Sciences (AIMS), district Muzaffarabad, AJK. All isolates were subjected to antibiotic susceptibility against seven commonly prescribed antibiotics by Kirby-Bauer disk diffusion method. However, all the E. coli isolates were susceptible to Imipenem. Eight percent and 10% of isolates from CMH and AIMS were found to be resistant against Nitrofurantoin drug respectively. Similarly, 94% and 74% isolates from CMH and 60% and 64% isolates from AIMS were found to be resistant against Co-trimoxazole and Coamoxiclave, respectively. Pipemedic acid resistance was also detected in 76% and 60% isolates from AIMS and CMH, respectively. Ciprofloxacin resistance was also observed in 54% and 36% isolates from AIMS and CMH, respectively. The finding of the study revealed that both hospitals have different drug susceptibility pattern against uropathogenic E. coli, which may be associated with patterns of drug prescription in empirical treatment of urinary tract infections. There is a vital need for appropriate development of hospital-specific antibiograms to determine appropriate empiric therapy of urinary tract infections.
Antibiotic susceptibility analysis of Escherichia coli as the cause of urinary tract infection
JPBIO (Jurnal Pendidikan Biologi)
The incidence of urinary tract infection (UTI) in Indonesia is still quite high. The UTIs are generally treated with antibiotic. However, the use of antibiotics has recently increased tremendously thus triggering bacterial resistance. This study was to analyze the susceptibility of the Escherichia coli as the cause of the UTI to 15 antibiotics at the UPTD Health Laboratory Center of Maluku Province. The study was an experimental laboratory study using the disk diffusion method. The antibiotic disc was placed on the media and the inhibition zone against E. coli was measured. The results showed that the E. coli was resistant (26.67%) to 4 types of antibiotics, namely amoxilin, ampicillin, sulfametazole dan penicillin. Meanwhile, E. coli was sensitive (46.66%) to 7 types of antibiotics, namely kanamycin, gentamicin, subactam ampicillin, chloramphenicol, amikacin, ceffazedine and azithromycin. Furthermore, E. coli was intermediate (26.67%) against 4 types of antibiotics, namely ciproflo...
Antimicrobial resistance is not only increasing the healthcare costs, but also the severity and death rates from certain infections that could have been avoided by prudent and rational use of the existing and newer antimicrobial agents. Prudent and rational use of antimicrobial is possible by forming local, national and global wide Antibiogram. The present study is undertaken to prepare local antibiogram and to discuss general issues related to antimicrobials use. Total 170 urine samples were processed for culture sensitivity testing. Identification of bacteria was done by gram staining. Isolation of the organisms was done by inoculation of sample on agar medium. After 24 hours of incubation each organism was identified on the basis of morphology of colony in culture media and biochemical reactions. Antibiotic sensitivity Testing was carried out on Muller Hinton Medium by Disc Diffusion Method following Kirby Bauer method.Out of 170 samples were processed, 136 organisms were isolated from urine which includes Escherichia coli (76), Klebsiella (34), Pseudomonas (12), and Staphylococci (14). E.coli is most common organism (55.9 %). E.coli is most sensitive to piperacillin/tazobactam and gatifloxacin. An attempt has been made in this study to recognize the E.coli infection in patients of tertiary care hospital, Surendranagar, Gujarat; to record the antibiogram and probable drug of choice for E.coli. Considering the antibiotic susceptibility testing, cost, side effects and many other factors, gatifloxacin should be preferred for E.coli infection for UTI.
Asian Journal of Pharmaceutical and Clinical Research, 2018
The study was designed to diffuse awareness on the prevalence of Escherichia coli as a causative agent of urinary tract infection (UTI) in Sokoto metropolis as well as to determine the susceptibility to commonly used antibiotics in Specialist Hospital Sokoto (SHS). This is also to raise awareness of the risk of giving antibiotics and their direct impact on the outcome analysis of UTIs. Methods: This study was conducted at SHS, and ethical approval to carry out the study was obtained from the Ethical Committee of the hospital. Informed consent was obtained from each participant. Early morning, mid-stream clean catch urine samples were collected by patients in sterile disposable containers. The antibiotic susceptibility of the isolates was determined against 10 commonly prescribed antibiotics in SHS using the modified Kirby-Bauer disc agar diffusion. Results: A total of 86 urine samples were analyzed over 2 months, and 34 were culture positive giving an isolation rate of 39.5%, while 48 were culture negative giving a rate of 55.8%, and 4 (4.7%) were undecided. A total of 16 isolates were E. coli (47.1%), while 18 accounts for others (52.9%). The results of antimicrobial susceptibility profile to 10 antibiotics showed that E. coli displayed high susceptibility to vancomycin (91.6%), followed by amikacin (89.2%) and then meropenem (88.0%), while high rate of resistance was found in nalidixic acid (81.2%), followed by co-trimoxazole (73.3%) and then norfloxacin (76.2%). Conclusion: When there is an adequate detection of E. coli and other uropathogens, it will aid in selecting the appropriate antimicrobial therapy and this will also serve as a means of infection control. This will go a long way in reducing the cost of treatment and threat of resistance as witnessed in the management of some uropathogens.
American Journal of Internal Medicine, 2018
Urinary Tract Infection (UTI) is very common in our day-today clinical practice. Among all the organisms Escherichia coli (E coli) is the most common but antimicrobial resistance becomes an alarming issue for UTI management now a days. Aim of this study is to assess the pattern of antimicrobial resistance to E coli among the UTI patients in Jashore, Bangladesh. This observational study was conducted from February, 2017 to January, 2018 in the district of Jashore, Bangladesh. We recruited 696 patients of both sex with UTI only infected by E coli. We had excluded the patients with UTI caused by other than E coli, female during menstruation, pregnancy, history of taking antibiotics within last 21 days, catheterization within 1 month and pelvic organ & genito urinary tract surgery within 6 months. Evaluation of antimicrobial resistance was done according to the standard bacteriological methods. Mean age of our study cases was 41.46±17.21 years with the range from 15 to 91 years. More than 85% participants were female with a sex ratio was 8:1.5. Reproductive age group ranged from 21 to 50 years was affected most commonly which constituted approximately two-third of our study. Our study revealed that the maximum antimicrobial resistance to E coli was Cotrimoxazole (95.0%), followed by Ceftazidime (75.7%), Gentamicin (70.3%), Amikacin (69.0%), Imipenam (58.9 %,), Cefixime (58.0%), Ciprofloxacin (57.3%), Azithromycin (56.0%), Cefuroxime (46.6%), Cefotaxime (37.4%), Ceftriaxone (35.2%), Meropenem (32.2%), Nitrofurantion (4.7%). With the high magnitude of antimicrobial resistance to E coli among the UTI patients even with extended generation of Cephalosporins, Carbapenams, Ciprofloxacin, Cotrimoxazole, Azithromycin and Aminoglycosides, our recommendation as first line empirical treatment option in UTI should be Nitrofurantoin due to low resistance pattern.
Sudan journal of medical sciences, 2022
Background: The irrational use of antibiotics for the treatment of urinary tract infections (UTIs) may lead to increased antimicrobial resistance among uropathogenic Escherichia coli (E. coli), as well as multidrug resistance worldwide, which will limit available treatment options for UTIs caused by these organisms. This study aimed to determine the resistance pattern of E. coli causing UTIs in outpatients and in-patients of Soba University Hospital. Methods: Data were collected from the laboratory records in the Department of Microbiology in Soba Teaching Hospital by using a predesigned checklist and then analyzed using the statistical package for social sciences. Bivariate analysis (Chisquare test) was used to compare between variables. Results: Out of the 231 E. coli urine cultures, 160 (69.3%) were collected from females. The results showed high resistance to ampicillin (92.4%), amoxicillin-clavulanic acid (83.3%), cephalexin (90.6%), cefuroxime (72%), ceftazidime (71%), ceftriaxone (72%), ciprofloxacin (68%), and co-trimoxazole (75.3%). Collectively, around 188 (81.4%) were multidrug-resistant. On the other hand, the sensitivities of E. coli isolates were 68.8%, 93.1%, 89.4%, and 100% to gentamicin, amikacin, nitrofurantoin, and carbapenems, respectively. Conclusion: The rate of E. coli resistance was observed to be high to the commonly prescribed drugs for UTIs, including ampicillin, amoxicillin/clavulanic acid, different cephalosporins, fluoroquinolones, and co-trimoxazole. However, E. coli showed lower resistance rates to nitrofurantoin, amikacin, and carbapenems. Thus, these drugs can be reserved for the empirical treatment of UTIs caused by E. coli.