APPLICATIONS OF HPTLC IN HERBAL DRUGS ANALYSIS (original) (raw)
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Phytochemical Fingerprinting through Computerized HPTLC System for Quality Control of Herbal Drugs
International Journal for Research in Applied Science & Engineering Technology, 2018
High performance thin layer chromatography (HPTLC) is a sophisticated instrumental technique based on the full capabilities of thin layer chromatography. The advantages of automation, scanning, full optimization, selective detection principle, minimum sample preparation, hyphenation, etc. enable it to be a powerful analytical tool for chromatographic information of complex mixtures of inorganic, organic, and bio-molecules. Development of chemical fingerprints using HPTLC is an effective tool for linking the chemical constituent's profile of the plant with botanical identity as well as qualitative and quantitative estimation of chemical and biochemical markers. It has the potential to determine authenticity and reliability of chemical constituent of herbal drugs and formulations.
Journal of AOAC INTERNATIONAL, 2003
In herbal medicinal products the entire herbal drug or an herbal drug preparation is regarded as the active pharmaceutical ingredient, regardless of whether constituents with defined therapeutic activity are known. In quality control and stability testing of herbal medicinal products, fingerprint chromatograms are used as powerful tools to evaluate and compare the composition of compounds in such products. To fulfill the International Conference on Harmonization and Good Manufacturing Practice-based regulatory requirements in pharmaceutical quality control, chromatographic fingerprint analysis needs to be validated. Based on a standardized methodology, this paper provides a comprehensive concept for evaluating validation parameters for planar chromatographic fingerprinting by considering the stationary phase, sample application, developing solvent, chromatogram development, plate labeling, derivatization, documentation, and chromatographic equipment. Validation parameters addressed ...
Chromatography Research International
As traditional Chinese medicine (TCM) is gradually accepted by many countries, people pay much attention to the quality of herbal medicines. Because of the significant variation in active components in them, the quality control of herbal medicines is a very important issue. Nowadays, high-performance liquid chromatography (HPLC) fingerprint spectra (FPS) are widely used in identification and quality control of herbal medicines. This paper will analyze the methodology and their application in identifying and evaluating herbal medicines by means of HPLC FPS, which includes simple comparing, clustering, principal component analysis (PCA), and similarity analysis methods.
HPTLC' an important tool in standardization of herbal medical product: A review
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The analysis and quality control of herbal medicines are moving a step ahead towards an integrative and comprehensive direction, in order to tackle the complex nature of herbal medicines. High-performance thin layer chromatography (HPTLC) is one of the sophisticated instrumental techniques for qualitative and quantitative analysis of the herbs and herbal drugs. This article emphasize on HPTLC based analytical method development and evaluation of validation characteristics.
'HPTLC’ an Important Tool for Quantification of Herbal Product: A Case Study
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Large number of population prefer the Ayurvedic or herbal medicines to treat the various ailments. Herbal medicines are available in three forms as raw plant material or processed plant material or medicinal herbal product but mixtures have several chemical components. Therefore, it is very important to know the specific component having potential for treatment. For qualitative and quantitative analysis of the herbs and herbal drugs, Highperformance thin layer chromatography (HPTLC) is one of the best techniques. Hence, a case study has performed to quantify vasicinone component from Adhatoda zeylanica.
Critical Factor That Governs a Successful TLC/ HPTLC Analysis of Herbal Medicinal Products (HMPS)
2013
Chromatography is one of the fast emerging tools by which the quality control and fingerprint of herbs can be maintained. Using this technique, the identification of various chemical markers of the herbal drugs can be easily done and it also helps to identify the same herbs in combination. Application of TLC/HPTLC methodology in testing of phytoconstituents from individual herbal drugs and fingerprint characteristic of the herbal plants are reviewed in this paper. Popularity of TLC/HPTLC analytical method for analysis of herbal drugs due to economic, rapid, simultaneously screening of large number of herbal samples and less time consuming methods. The different mobile phase, spraying reagent, property of herbal drugs and its phytoconstituents, TLC/HPTLC plates, trouble shooting of HPTLC, different developing solvents and chromatograms are pointed out in this paper.
HPTLC in Herbal Drug Quantification
2011
For the past few decades, compounds from natural sources have been gaining importance because of the vast chemical diversity they offer. This has led to phenomenal increase in the demand for herbal medicines in the last two decades and need has been felt for ensuring the quality, safety, and efficacy of herbal drugs. Phytochemical evaluation is one of the tools for the quality assessment, which include preliminary phytochemical screening, chemoprofiling, and marker compound analysis using modern analytical techniques. High-performance thin-layer chromatography (HPTLC) has been emerged as an important tool for the qualitative, semiquantitative, and quantitative phytochemical analysis of the herbal drugs and formulations. This includes developing TLC fingerprinting profiles and estimation of biomarkers. This review has an attempt to focus on the theoretical considerations of HPTLC and some examples of herbal drugs and formulations analyzed by HPTLC.