Identification of Romanian Vitis vinifera L. cultivars in must using nuclear microsatellite markers (original) (raw)
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Molecular characterisation of Romanian grapevine cultivars using nuclear microsatellite markers
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture, 2013
In the present study we use six nuclear SSR loci (SS2, MD5, MD7, MD27, ZAG 62 and ZAG 79) to characterize four autochthonous grapevine cultivars (BAƒbeasca neagrAƒ, Feteasca regalCŽ, FrA¢ncuAŸa, and Grasa de Cotnari), including four international comparative genotypes (Cabernet Sauvignon, Chardonnay Blanc, Riesling Italian and Merlot Noir). The DNA microsatellite analysis was used to construct a barcode system. The advantage of this mode of grouping data is a visual representation of the number and size of alleles, allowing easy detection of genotypic differences between analysed cultivars. Our results shows that this system of data grouping can be useful for characterisation of Romanian cultivars at molecular level. The integration of such DNA barcodes into nationally and internationally coordinated databases could increase the accuracy with which grapevine genetic resources are managed in Romania.
Genotyping of Bulgarian Vitis vinifera L. cultivars by microsatellite analysis
Vitis -Geilweilerhof-
A characterization of the Bulgarian grapevine genepool (Vitis vinifera L. cultivars) was initiated through microsatellite analysis. Seventy four wine and table grape-vine varieties from the National List of Cultivars, were analyzed at 9 microsatellite loci: VVS2, ssrVvUCH11, ssrVvUCH 29, ssrVrZAG21, ssrVrZAG47, ssrVrZAG62, ssrVrZAG64, ssrVrZAG79 and ssrVrZAG83. The high genetic diversity (78 %) allowed accurate identification and discrimination of the cultivars. The low PI value (1.201 x 10 -8) reflects the high discriminative power of the chosen set of markers for the investigated population. Based on the microsatellite allele data, two pairs of old native vari-eties, Misket Cherven and Misket Vrachanski; Tamyanka and Tamyanka tvarda, were considered distinct cultivars. The synonymy of (i) Tamyanka, Italian Moscato Bianco and Greek Moschato Kerkyras and (ii) Pamid and Greek Pamidi was verified, while the putative synonymy of Mavrud and Greek Mavroudi Arachovis was rejected. Further...
Analysis of grapevine ( Vitis vinifera L.) varieties by molecular markers
The aim of our work was to investigate the genome of grapevine with molecular markers (isoenzyme, SSR). The isoenzyme patterns of 4 enzyme systems (CO, GOT, AcP, PER) and the microsatellite profile in 6 loci (VVS2, VVS16, VVMD7, VMC4A1, VMC4G6, VrZag79) of 48 grapevine varieties were analysed. In the case of the CO, GOT, AcP and PER enzymes the results were reproducible and the patterns of the woody stems were independent from the time of sampling in the resting period of the grape. Based on the isoenzyme patterns of these 4 enzymes the most of the investigated 48 varieties (40 varieties) were identifiable. A correlation was found between the isoenzyme patterns and the pertain to convarietas of the varieties. It was established, that while the varieties of the convarietas pontica differentiate from the varieties of the convarietas orientalis and occidentalis, the last two groups don't differentiate strongly from each other. A special acid phosphatase isoenzyme banding pattern was identified, which is characteristic for the pontican cultivars, but it seldom appears in other two groups. Based on the microsatellite analyses 46 varieties from the 48 investigated ones were identifiable. It was possible to differentiate 2 cultivars of the Pinot conculta, the 'Pinot blanc' and the 'Pinot gris' by the microsatellite analyses in the VMC4A1 locus.
Genetic profiling of nine grapevine cultivars from Romania, based on SSR markers
Romanian …, 2010
In the present study, a genetic characterization of nine grapevine cultivars, from the National Institute for Biotechnologies in Horticulture, Ştefăneşti-Argeş collection, was carried out, based on 15 microsatellite markers. These are among the most important cultivars encountered in Romanian vineyards, four of them non-autochtonous -Muscat Ottonel, Italian Riesling, Cabernet Sauvignon, Sauvignon, and five of Romanian origin -Tămâioasă Românească, Negru aromat, Fetească albă, Fetească regală, Fetească neagră.
The Current State of Characterization of Romanian Vitis vinifera L. Germplasm by Molecular Markers
In recent years, the concerns of some research teams have focused in development an international strategy for the germplasm collections characterization of important horticultural species, as response to the European Union initiative for inventory and conservation of genetic resources. In this context, the European Union has developed international research projects having as main aim collecting the data and improving knowledge regarding conservation and sustainable use of Vitis genetic resources in Europe. Romania has a multimillenary tradition in grapevine cultivation and wine production. Considering this, it is necessary to use ef icient and reliable methods for the accurate identi ication of autochthonous and newly created grapevine cultivars included in national germplasm collections. The intensive renewals of grapevine plantations, implementation of EU regulations and reshaping of the national viticulture and wine industries, that take place at present in Romania, require application of modern methods for the accurate identi ication of valuable cultivars. In this paper, the advantages of using DNA markers in identi ication and characterisation of grape varieties included in Romanian germplasm collections were discussed. The aim of this review was to highlight the importance of molecular markers usage in the studies related the genetic diversity characterisation of this important species.
Molecular Biotechnology, 2014
The present work report the characterization of twenty-one table grapes candidate cultivars plus five registered ones included as reference, by means of 47 ampelographic traits, 23 ampelometric measurements and six microsatellite loci. The final goal of the research was to analyse the possibility of reducing the number of morphological and molecular tools required for a precise and effective description of a grape genotype or cultivar. This would be of great help for future biodiversity description on a larger sample of more than 300 table grapes accessions today grown at the 'Consiglio per la Ricerca e la sperimentazione in Agricoltura (C.R.A.)-Unità di ricerca per l'uva da tavola e la vitivinicoltura in ambiente mediterraneo (Bari-Italy)'. OIV ampelographic traits showed a clear distinction among all twenty-six genotypes analysed, suggesting the relevant morphological variability investigated. Principal component analysis based on ampelometric traits revealed main veins ON 3 , ON 4 and O 3 N 4 ; ratios between main veins; angles between main veins and of petiolar sinus, to be the most effective records in differentiating cultivars, for a total variation of 69.9 % described by the first three components. Molecular analysis based on six microsatellite loci was performed on all genotypes, providing a detailed molecular profile and a dendrogram of genetic similarity, in which all genotypes were clearly distinguishable. Finally, with the goal of using the minimum possible number of markers to differentiate genotypes, microsatellites VVMD5 and VVMD27 were selected to be sufficient to distinguish among all the candidate cultivars included in the analysis, representing a possible 'step by step' approach when a molecular characterization has to be undertaken on a large number of genotypes, by first testing few markers and increasing their number only if necessary. Consiglio per la Ricerca e la sperimentazione in Agricoltura -CRA, Unità di ricerca per l'uva da tavola e la vitivinicoltura in ambiente mediterraneo,
Microsatellite fingerprinting of grapevine (Vitis vinifera L.) varieties of the Carpathian Basin
2015
Altogether 101 Vitis vinifera L. genotypes were analysed at 6 microsatellite loci (Scu8vv, Scu10vv, VVMD21, VVMD36, ssrVRZAG64, ssrVRZAG79). Ninety-seven were autochthonous accessions of the Carpathian Basin and 4 were international cultivars. The allele composition and sizes obtained with the 6 microsatellite primer pairs were appropriate for discrimination of 95 cultivars. Berry colour-variants of cvs Gohér (Gohér fehér-white and Gohér piros-red), Lisztes (Lisztes fehér and Lisztes piros) as well as the cvs Bakator (Bakator piros and Bakator tüdőszín - light red) were exceptions.
Molecular characterization of old local grapevine varieties from South East European countries
Vitis -Geilweilerhof-
South East European (SEE) viticulture partially relies on native grapevine varieties, previously scarcely described. In order to characterize old local grapevine varieties and assess the level of synonymy and genetic diversity from SEE countries, we described and genotyped 122 accessions from Albania, Federation of Bosnia and Herzegovina (B&H), Croatia, Macedonia, Moldova, Montenegro, Republika Srpska (Bosnia and Herzegovina) and Romania on nine most commonly used microsatellite loci. As a result of the study a total of 86 different genotypes were identified. All loci were very polymorphic and a total of 96 alleles were detected, ranging from 8 to 14 alleles per locus, with an average allele number of 10.67. Overall observed heterozygosity was 0.759 and slightly lower than expected (0.789) while gene diversity per locus varied between 0.600 (VVMD27) and 0.906 (VVMD28). Eleven cases of synonymy and three of homonymy have been recorded for samples harvested from different countries. Cultivars with identical genotypes were mostly detected between neighboring countries. No clear differentiation between countries was detected although several specific alleles were detected. The integration of the obtained genetic data with ampelographic ones is very important for accurate identification of the SEE cultivars and provides a significant tool in cultivar preservation and utilization.
Tarım Bilimleri Dergisi, 2015
Thirty-six autochthonous grapevine cultivars from the Aegean region of Turkey in addition to standard cultivars Cabernet Sauvignon and Merlot (Vitis vinifera L.) were fingerprinted using SSR markers to assess their genetic relationships. Eleven SSR primers produced successful amplifications and yielded 37 polymorphic bands. The number of bands per primer changed between 2 and 6 while the number of polymorphic bands was between 2 and 3.6. Dice genetic similarity coefficients ranged between 0.296 and 0.882 among the genotypes. The UPGMA dendrogram showed two main groups. The first group was composed of Sultan Dimriti, Veyis and Güvercin Gözü cultivars. A large number of subgroups were placed in the second group which included the majority of autochthonous cultivars. The genetic differences among the autochthonous cultivars and reference cultivars were clearly observed. The results showed that cultivars Siyah Razakı and Parmak (0.96) were the most similar ones. Synonyms were identified for İnek Memesi and Ufak Dimrit cultivars. In addition, homonymous cultivars were detected. SSR markers have proved to be an efficient tool for fingerprinting grapevine cultivars and conducting genetic diversity studies.