Aerococcus sanguicola sp. nov., isolated from a human clinical source (original) (raw)
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Aerococcus urinaehominis sp. nov., isolated from human urine
International Journal of Systematic and Evolutionary Microbiology, 2001
Phenotypic and phylogenetic studies were performed on an unknown Grampositive catalase-negative coccus isolated from human urine. Comparative 16S rRNA gene sequencing demonstrated that the organism represents a new subline within the genus Aerococcus. The unknown bacterium was readily distinguished from the three currently recognized Aerococcus species, Aerococcus christensenii, Aerococcus urinae and Aerococcus viridans, by biochemical tests and electrophoretic analysis of whole-cell proteins. Based on phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium from urine be classified as Aerococcus urinaehominis sp. nov. The type strain of Aerococcus urinaehominis is CCUG 42038b T (l CIP 106675 T).
Aerococcus suis sp. nov., isolated from clinical specimens from swine
International Journal of Systematic and Evolutionary Microbiology, 2007
Biochemical and molecular genetic studies were performed for five isolates of unknown Gram-positive, catalase-negative, cocci-shaped micro-organisms obtained from clinical samples from pigs. The micro-organisms were tentatively identified as Aerococcus species on the basis of the results from cellular morphological and biochemical tests. 16S rRNA gene sequencing studies confirmed the provisional identification of the isolates as members of the genus Aerococcus, but the micro-organism did not correspond to any recognized species of this genus. The nearest phylogenetic relatives of these unknown cocci isolated from pigs were Aerococcus viridans (95.9 % 16S rRNA gene sequence similarity) and Aerococcus urinaeequi (95.8 %). The unknown bacterium, however, was distinguishable from these two species and from other animal aerococci by using biochemical tests. On the basis of both phenotypic and phylogenetic findings, the isolates represent a novel species of the genus Aerococcus, for which the name Aerococcus suis sp. nov. is proposed. The type strain is 1821/02 T (=CECT 7139 T =CCUG 52530 T ).
Aerococcus -like organisms: use of antibiograms for diagnostic and taxonomic purposes
Journal of Antimicrobial Chemotherapy, 1996
Recently, some Aerococcus-like organisms (ALOs), isolated from urine and blood of elderly patients with urinary tract infection, have been described. In this study ALOs and related taxons were tested for susceptibility by agar diffusion and agar dilution methods to 15 selected antimicrobial agents for diagnostic and taxonomic considerations. ALOs were susceptible to a wide range of antimicrobials including /?-lactams, but resistant to aminoglycosides, sulphonamides, trimethoprim and nalidixic acid. By using tablets containing vancomycin, furazolidone and bacitracin, it was possible to separate ALOs from related taxons. Clustering based on antibiotic susceptibilities showed that there is little similarity between Aerococcus viridans and ALOs.
Bacteremia with Aerococcus sanguinicola: Case Series with Characterization of Virulence Properties
Open forum infectious diseases, 2014
Since Aerococcus sanguinicola was designated as a species in 2001, only a few cases of bacteremia have been reported. The aim with this study was to describe the clinical presentation of A sanguinicola bacteremia and to determine the antibiotic susceptibility and the capacity of the bacteria to form biofilm and to induce platelet aggregation. Isolates of A sanguinicola from blood cultures were retrospectively identified from 2 clinical microbiology laboratories for 2006 to 2012. Species identity was confirmed through sequencing of the 16S rRNA gene. The medical charts of patients were reviewed. The minimum inhibitory concentration (MIC) for relevant antibiotics was determined. Biofilm formation was measured as the amount of crystal violet absorbed. Platelet aggregation was determined by aggregometry. Eleven cases of A sanguinicola bacteremia were identified. All patients were male and the median age was 82 years (range 67-93). Nine patients fulfilled criteria for severe sepsis, and ...
Microbial Pathogenesis
Bacterial adhesion gene homologs were identified in A. sanguinicola (htpB, fbpA, lmb, and ilpA) and A. urinae (htpB, lap, lmb, fbp54, and ilpA) genomes. Capsular polysaccharide (CPS) gene homologs were identified in A. sanguinicola (15 genes) and A. urinae (11-16 genes) strains, giving rise to one and five types of putative CPS loci, respectively. Marked differences were observed within A. urinae 1984-2004 and 2010-2015 strains in regards to genome sizes, core-genomes, proteome conservations, and phylogenetic analysis.
Urinary tract infection by Aerococcus sanguinicola. An emerging opportunistic pathogen
Revista Clínica Española (English Edition), 2018
Elderly patients with underlying urological disease have a greater risk of urinary tract infections due to uncommon pathogens. The disease caused by Aerococcus has been underestimated, but mass spectrometry could be a simple method for identifying this pathogen. In this study, we report 2 cases of urinary tract infection by Aerococcus sanguinicola. A descriptive clinical-microbiological study was conducted on the presence of A. sanguinicola causing urinary tract infections. The presence of A. sanguinicola occurred in elderly patients with previous urological disease and a significant count in urine obtained through bladder catheterization. Correct identification was achieved through mass spectrometry, and the clinical outcome of administering amoxicillin and cefuroxime was satisfactory. In this study, we also report the pathogenic capacity of A. sanguinicola. When there is a significant number of alpha-hemolytic microorganisms in the urine cultures, A. sanguinicola should be ruled out before reporting a result as urogenital microbiota.
Aerococcus-iike organisms: use of antibiograms for diagnostic and taxonomic purposes
2015
Recently, some Aerococcus-like organisms (ALOs), isolated from urine and blood of elderly patients with urinary tract infection, have been described. In this study ALOs and related taxons were tested for susceptibility by agar diffusion and agar dilution methods to 15 selected antimicrobial agents for diagnostic and taxonomic considerations. ALOs were susceptible to a wide range of antimicrobials including /?-lactams, but resistant to aminoglycosides, sulphonamides, trimethoprim and nalidixic acid. By using tablets containing vancomycin, furazolidone and bacitracin, it was possible to separate ALOs from related taxons. Clustering based on antibiotic susceptibilities showed that there is little similarity between Aerococcus viridans and ALOs.
Identification of Aerococcus urinae in urine samples
Clinical Microbiology and Infection, 2003
To evaluate procedures for the identi®cation of Aerococcus urinae, we examined 24 a-hemolytic non-enterococcal bacterial isolates from 4373 urine samples. Published procedures were compared with 16s rRNA sequencing and biochemical pro®ling (BBL-Crystal-GP). 16s rRNA sequencing and BBL-Crystal-GP identi®ed the same 13 isolates as A. urinae. Published tests failed to distinguish the 13 A. urinae isolates from eight non-A. urinae isolates; several tests exhibited no discrimination. Cipro¯oxacin and trimethoprim susceptibility and growth at 45 8C improved discrimination. For urinary isolates, standard procedures for identi®cation of A. urinae are redundant and insuf®ciently discriminatory, and may need revision. BBL-Crystal-GP is an accurate alternative.
Aerococcus urinae in urinary tract infections
Journal of clinical microbiology, 2000
Aerococcus urinae is a rarely reported pathogen, possibly due to difficulties in the identification of the organism. A. urinae is a gram-positive coccus that grows in pairs and clusters, produces alpha-hemolysis on blood agar, and is negative for catalase and pyrrolidonyl aminopeptidase. Some of these characteristics and its being absent from the databases of most commercial identification systems could allow A. urinae to be misidentified as a streptococcus, enterococcus, or staphylococcus. We report two cases of urinary tract infection (UTI) caused by A. urinae and characterize these isolates by morphology, biochemical testing, whole-cell fatty acid analysis, 16S rRNA gene sequencing, and antibiotic susceptibilities. Most patients infected with A. urinae are elderly males with predisposing conditions who present initially with UTI. Because A. urinae is resistant to sulfonamides, treatment could be inappropriate, with infections resulting in serious complications, including death. I...