Effects of Vernonia amygdalina methanol leaf extract and fractions on Ascaridia galli in experimentally infected birds with regard to its pathological effect (original) (raw)

Bulletin of the National Research Centre

Background Helminthiasis, usually a chronic problem in poultry, is the major cause of economic losses in the poultry industry. This study was undertaken with the aim of evaluating the in vitro and in vivo anthelmintic effects of Vernonia amygdalina methanol leaf extract and fractions on Ascaridia galli in experimentally infected birds. Standard protocols for extraction, phytochemical screening, isolation and infection of parasite and histological examination were followed. Result At 50 mg/ml albendazole (ALB) caused the highest inhibition of embryonation (97.3%) followed by crude methanol extract (CME) (94.5%), ethyl acetate fraction (EAF) (81.3%) and butanol fraction (BF) (80.3%). The lowest faecal egg counts (FEC) and highest FEC at 21 days post-treatment were observed in the group treated with albendazole (99.2%) and CME at 800 mg/kg (96.9%). Deparasitization at 200 mg/kg caused by ALB was (93.8%), while at 200, 400 and 800 mg/kg, CME was (77.5%, 87.5% and 91.86%), BF was (75.51%...