Characterization of Purified Bacteriocin (Plantaricin and Acidocin) Produced from Lactobacillus Isolates and Study its Effects Against Growth Pathogenic Bacteria (original) (raw)

The plantaricin and acidocin production was induced by adding the mutagenic agent Mitomycin C. The physical characteristics were also studied and the results showed that the molecular weight of plantaricin and acidocin were 14.13, 31.62 KD .respectively by gel filtration chromatography. Plantaricin activity was stable at pH values (3-9) but 50% of its activity was lost at pH 10, while in acidocin activity was stable at pH values (3-7) but 50% of its activity was lost at pH 9. Plantaricin showed high thermostability at different temperatures (50-75)°C for (10-60) min , it remained active after being treated with 100°C for (10-30) min, but it retained only 50% of its activity after exposure to 100°C for (60) min and autoclaving at (121°C for 15 min), while acidocin showed high thermostability at different temperatures (50-100)°C for (10-60) min , but it retained only 50% of its activity after treatment at autoclaving at (121°C for 15 min).The antibacterial activity was observed that c...