Yeast transformation and horisontal gene transfer (original) (raw)

1997

Abstract

In contrast to the vertical inheritance of genetic traits, from parents to their offspring, the acquisition of genetic material from neighbors may involve members of different species. The incoming genetic material has first to find a way to enter the recipient cell and then to become established there. Two examples of such horisontal gene transfer acquired much attention due to their great practical importance: plasmid shuttling between bacterial species and unidirectional transfer of genetic information from the procaryote Agrobacterium tumefaciens to the plant cells where the single-stranded DNA from bacterial origin integrates in the plant genome by illegitimate recombination. The exogenous DNA, coming from another species, is expected to have no homology, or only limited homology to the host genome. How could such heterologous DNA integrate into the genome of the yeast Saccharomyces cerevisiae, whose recombinational machinery is known to operate efficiently only with homologous...

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