Distribution of serovars and antibiotic resistance genes of Salmonella isolated from chicken gizzards in Abidjan, Côte d'Ivoire (original) (raw)

2015, International Journal of Innovation and Applied Studies

Salmonella enterica is a pathogen of humans and animals, and is the most frequent causes of foodborne illness worldwide. The increasing prevalence of multidrug resistance among Salmonella isolates from retail meat such as chicken gizzards has been an emerging problem in Côte d'Ivoire. 66 batches of raw chicken gizzard were collected to determine Salmonella prevalence. Serotyping, antimicrobial susceptibility, presence of tet(A), qnr(A, B and S), Sul1, bla CTX-M 1 genes were researched. Salmonella strains obtained by the method of culture were 104 (77.27 %). Distinct serovars obtained was: Derby (18.9 %), Budapest (17 %), Essen and Kentucky (11.3 %), Hadar (9.4 %), Agona (7.5 %), Chester, Riuru and Schwarzengrund (3.8 %), Aoto, Bargny, Elisabethville, Fortune, Poeselderf and Santiago (1.9 %). All isolates, exhibited resistance to one or more antimicrobial agents used. The antibiotic resistance was mainly detected against, Cefotaxim (0.96 %), Cefalotin (9.62 %), Amoxicillin/Clavulanic acid (7.69 %), Amoxicillin (7.69 %), Gentamycin (10.58 %), Ciprofloxacin (30.77 %), Chloramphenicol (29.81 %), Nalidixic acid (35.58 %), Tircacillin (46.15 %), Tetracyclin (73.08 %) and Cotrimoxazol (93.37 %). 65 isolates (63.1 %) were resistant to more than 3 antibiotic families (ß-lactams, quinolones, cyclines, sulfonamides). The detection by PCR of 20 isolates highlighted the presence of 3 different genes (tet (A), bla CTX-Mgroup1 and sul 1 ) . Our findings showed that raw chicken gizzards could be considered as an important source of transmission of salmonellosis to human. Furthermore, the presence of resistance genes exhibited by the strains observed in this study could be a public health problem.