Matrix Matalloproteinase-8 Gene Polymorphism in Chronic Periapical Lesions (original) (raw)
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Gene Polymorphism of Matrix Metalloproteinase-1 in Chronic Periapical Lesions
Dentistry, 2017
in aberrant connective tissue destruction. Expression of MMPs is regulated primarily at the transcriptional level where the promoter of the genes responds to different regulators [8]. Some functional polymorphisms have been described in the regulatory region of MMPs genes [5]. Because destructive enzymes of the MMPs family are involved in this process, allele polymorphisms of MMPs genes are interesting candidates for analyses of the influence, susceptibility and severity of chronic periapical lesions (CPLs). Subfamily of human colagenases has three members, from which MMP-1 (fibroblast type of colgenase, colagenase-1) is one of our interest [9]. Gen of the MMP-1 is localized on chromosome 11q22 and its expression was shown in various types of healthy cells (stromal fibroblasts, macrophage, endothelial and epithelial cells), as well as in different inflammatory and carcinoma cells [10]. Level of expression of MMP-1 can be under the influence of different single nucleotide polymorphism in promontory region. Insertion or deletion of the guanine from the position-1607 can be identified in humans in promoter of the gen of MMP-1 developing two
+17 C/G polymorphism in matrix metalloproteinase (MMP)-8 gene and its association with periodontitis
Journal of Stomatology
Introduction: Matrix metalloproteinase-8 (MMP-8), encoded by MMP-8 gene (chromosomal location, 11q22.3), is a proteolytic enzyme that is involved in the pathogenesis of periodontitis. Polymorphism of the encoding MMP-8 gene can affect the risk of the disease. Objectives: This study aimed to evaluate the relationship between +17 C/G polymorphism in MMP-8 and periodontitis in an Indonesian sample population. Material and methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the +17 C/G polymorphism in MMP-8 was conducted in stored genomic DNA samples from 100 subjects with periodontitis and 100 healthy controls. Patients were examined in a previous study by a periodontics and diagnosed as periodontitis based on anamnesis and clinical examination (periodontal pocket depth ≥ 4 mm and the presence of clinical attachment loss. Results: The frequencies of the genotypes or alleles in the periodontitis and control groups were not significantly different (genotype, p = 0.283; alleles, p = 0.11). Any remaining trend toward higher frequencies of the polymorphic genotypes or alleles in the periodontitis group was explained by the adjustment for age. Conclusions: The results showed no significant association between the +17 C/G polymorphism in MMP-8 and the risk of periodontitis in the Indonesian population. No change in the significance is expected even with a considerably larger sample.
www.scielo.br/jaos http://dx. I ncreased matrix metalloproteinases (MMPs) activity is a hallmark of periapical granulomas. However, the factors underlying the MMPs expression modulation in healthy and diseased periapical tissues remains to be determined. Objective: In this study, we evaluated the milieu elements with MMP-1 mRNA levels in vivo. Material and Methods: MMP1-1607 SNP and the mRNA levels of MMP-1, TNF-, IFN-, IL-17A, IL-21, IL-10, IL-4, IL-9, and FOXp3 were determined via RealTimePCR in DNA/RNA samples from patients presenting periapical granulomas (N=111, for both genotyping and expression analysis) and control subjects (N=214 for genotyping and N=26 for expression analysis). The Shapiro-Wilk, Fisher, Pearson, Chi-square ordinal least squares regression tests were used for data controls, comprising a risk factor for periapical lesions development. MMP-1 mRNA levels were higher in periapical lesions than in healthy periodontal ligament samples, as well as higher in active than in inactive lesions. The polymorphic allele 2G carriers presented a genetic polymorphism and the expression levels of MMP-1. Additionally, the pro-and anti-, TNF-as complementary explanatory variables of MMP-1 expression. Conclusion: The MMP1-1607 association with increased MMP-1 mRNA levels in periapical lesions. The MMP-1 expression , IL-21, IL-17A, and IFN-g associated with increased MMP-1 levels in periapical lesions, while IL-10, IL-9, or IL-4 presented an inverse association.
Journal of Clinical Periodontology, 2008
Aim: Matrix metalloproteinase-1 (MMP-1) is a proteolytic enzyme that degrades extracellular matrix and plays a fundamental role during destruction of periodontal tissues. The aim of this study was to examine the association between MMP-1 À 1607 1G/2G polymorphism and chronic periodontitis susceptibility in a Turkish population. Material and Methods: A total of 180 subjects were enrolled in this study. All the subjects received a periodontal examination including full-mouth clinical attachment loss measurements, probing depths, plaque index scores, gingival index scores and radiographic bone loss ratios. Three groups formed according to periodontal conditions were healthy, moderate periodontitis and severe periodontitis groups. MMP-1 À 1607 1G/2G gene promoter polymorphism was genotyped using a polymerase chain reaction-restriction fragment length polymorphism method. Results: Analysis of the polymorphism showed no differences in distribution of the MMP-1 À 1607 1G/2G polymorphism among healthy, moderate periodontitis and severe periodontitis groups (p40.05). When the groups were further stratified by smoking status, we found no significant differences in genotype distributions, allele frequencies and carriage rates among any groups either (p40.05). Conclusions: On the basis of the results, no significant association is found for the MMP-1 À 1607 1G/2G polymorphism with susceptibility to periodontitis. Moreover, smoking status did not seem to affect this result.
Journal of Applied Oral Science, 2016
I ncreased matrix metalloproteinases (MMPs) activity is a hallmark of periapical granulomas. However, the factors underlying the MMPs expression modulation in healthy and diseased periapical tissues remains to be determined. Objective: In this study, we evaluated the milieu elements with MMP-1 mRNA levels in vivo. Material and Methods: MMP1-1607 SNP and the mRNA levels of MMP-1, TNF-, IFN-, IL-17A, IL-21, IL-10, IL-4, IL-9, and FOXp3 were determined via RealTimePCR in DNA/RNA samples from patients presenting periapical granulomas (N=111, for both genotyping and expression analysis) and control subjects (N=214 for genotyping and N=26 for expression analysis). The Shapiro-Wilk, Fisher, Pearson, Chi-square ordinal least squares regression tests were used for data controls, comprising a risk factor for periapical lesions development. MMP-1 mRNA levels were higher in periapical lesions than in healthy periodontal ligament samples, as well as higher in active than in inactive lesions. The polymorphic allele 2G carriers presented a genetic polymorphism and the expression levels of MMP-1. Additionally, the pro-and anti-, TNFas complementary explanatory variables of MMP-1 expression. Conclusion: The MMP1-1607 association with increased MMP-1 mRNA levels in periapical lesions. The MMP-1 expression , IL-21, IL-17A, and IFN-g associated with increased MMP-1 levels in periapical lesions, while IL-10, IL-9, or IL-4 presented an inverse association.
Pathogens, 2021
Introduction: Periodontitis is characterized by the destruction of tooth-supporting tissues. Matrix metalloproteinases (MMPs) play a significant part in the degradation of collagen structure. The gingival crevicular fluid (GCF) levels of MMPs increase with the progression of periodontal inflammation. Polymorphisms can be responsible for high expression of MMPs and can exacerbate the breakdown of collagen structure. This study aims to investigate the effect of MMP-3 -1171 5A/6A polymorphism and the GCF levels of MMP-3 in a group of Turkish periodontitis patients. Materials and Methods: Non-smoking, stage II grade A periodontitis (S II-Gr A) (n = 68) and stage II grade B periodontitis (S II-Gr C) (n = 64) patients were recruited. Healthy individuals (H) (n = 72) without signs of gingivitis or periodontitis served as the control. Venous blood was collected from participants to obtain DNA, and the polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) method was u...
International Journal of Applied Pharmaceutics
Objective: This study aimed to identify the distribution of matrix metalloproteinase (MMP)-1 −1607 1G/2G alleles and genotypes in subjects withchronic periodontitis and healthy subjects in a sample of Indonesian population and assess the possible association of this polymorphism withsusceptibility to chronic periodontitis.Methods: Genomic DNA samples were obtained from 200 Indonesian males aged 33–78 years old, comprising 100 chronic periodontitis patientsand 100 healthy controls. DNA fragments were amplified by a polymerase chain reaction and analyzed by restriction fragment length polymorphism.Results were analyzed by Chi-square test.Results: The frequency of the 2G allele was high both in subjects with periodontitis (87%) and in controls (91%). Analysis of MMP-1 genotype(−1607 1G/2G) showed no significant difference between the chronic periodontitis and healthy groups (p>0.05).Conclusion: The result found no association between MMP-1 −1607 1G/2G polymorphism and susceptibility...
2017
The aim of this research was to compare the distribution of the MMP-9 -1562 C/T (rs3918242) polymorphism in Indonesian males with and without periodontitis. This descriptive study used 100 stored biological samples. The variation in the MMP-9 (-1562 C/T) polymorphism was investigated by the polymerase chain reaction (PCR) – restriction fragment length (RFLP) method with SphI restriction enzyme digestion and electrophoresis of the resulting fragments. A significant difference was found for MMP-9 -1562 C/T genotypes and alleles between subjects with periodontitis and healthy controls (p < 0.05). The T allele was relatively uncommon, as it appeared in 2% of healthy controls, with no TT genotype observed in subjects with or without periodontitis. Nevertheless, the T allele and CT genotype appeared to be significantly associated with the risk of periodontitis. The results suggested that the T allele of the MMP-9 -1562 C/T polymorphism is significantly associated with periodontitis. . ...
Iranian Red Crescent Medical Journal, 2019
Background: An imbalance in the expression of matrix metalloproteinases (MMPs) and tissue inhibitor metalloproteinases initiate the destructive process in chronic periodontitis (CP). C-reactive protein (CRP) is a systemic inflammatory mediator that reflects an acute immune response. Objectives: The purpose of this investigation was to analyze the association between the MMP-1-1607 1G/2G (rs1799750) and CRP 717 A/G (rs2794521) gene polymorphisms and chronic periodontitis in Iran. Methods: This analytical case-control study was performed among 141 participants including 63 CP cases and 78 matched healthy individuals. Five milliliters of peripheral blood was collected for DNA isolation. Restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) was performed for single-nucleotide polymorphism (SNP) analysis. The frequencies were analyzed by chi-squared test (95% CI, P < 0.05). In addition, genetic data were assessed by the Hardy-Weinberg principle, linkage disequilibrium, and haplotype analysis. Results: Our findings presented no significant relationship between genotype/alleles of MMP-1-1607 1G/2G (rs1799750) (0.73: 0.27-1.95, P = 0.48) or CRP 717 A/G (rs2794521) (0.384: 0.104-1.414, P = 0.303) and the presence of CP (P = 0.47 and P = 0.30, respectively). The analysis of genetic distribution among various severities of CP and controls revealed no significant association between various severities of CP and MMP-1-1607 1G/2G (rs1799750) (P = 0.52) and CRP 717 A/G (rs2794521) (P = 0.67). Conclusions: Our results suggest no association between the occurrence or severity of chronic periodontitis and MMP-1-1607 1G/2G (rs1799750) and CRP 717 A/G (rs2794521) polymorphisms. Further studies with larger sample sizes may provide a more generalizable evidence-based overview of the relationship between these gene polymorphisms and periodontitis.