Renal Accumulation of 99m TC-Dmsa in the Artificially Perfused Isolated Rat Kidney (original) (raw)

Abstract

I~ _o:der to investigate aspects of the renal handling of 99 mTc-DMSA, 68 isolated rat kidneys were artificially perfused. The experimental groups were: Group 1 (no. = 32)-oxygenated filtering kidneys; Group 2 (no. = 29)-oxygenated non-filtering kidneys; Group 3 (no. = 7)-anaerobic non-filtering kidneys. We conclude that the 99 mTc-DMSA complex is strongly bound to albumin is not filtered and is removed from perfusion fluid through the renal peritubular capillary rout~ and that this occurs by an active process which depends upon aerobic metabolism. This process has a high capacity and is not inhibited by probenecid.

Loading...

Loading Preview

Sorry, preview is currently unavailable. You can download the paper by clicking the button above.

References (24)

  1. Dahl, D. S., O'Connor, V. J., Jr., Walker, C. D. and Simon, N. M.: The morbidity of differential renal function studies. JAMA, 202: 857, 1967.
  2. Lin, T. H., Khentigan, A. and Winchell, H. S.: A 99 mTc-chelate substitute for organoradiomercurial renal agents. J. Nucl. Med., 15: 34, 1974.
  3. Erlander, D., Weber, P. M. and dos Remedios, L. V.: Renal cortical imaging in 35 patients: superior quality with 99 mTc-DMSA. J. Nucl. Med., 15: 743, 1974.
  4. Kawamura, J., Hosokawa, S., Yoshida, 0., Fujita, T., Ishii, Y. and Torizuka, K.: Validity of 99 mTc dimercaptosuccinic acid renal uptake for an assessment of individual kidney function. J. Urol., 119: 305, 1978.
  5. Daly, M. J. and Henry, R. E.: Defining renal anatomy and function with 99 m technetium dimercaptosuccinic acid: clinical and reno- graphic correlation. J. Urol., 126: 5, 1981.
  6. Goldraich, N. P., Goldraich, I. H., Anselmi, 0. E., Ludwig, E. B. and Ramos, 0. L.: Assessment of individual renal function with DMSA renal scan in children with primary vesicoureteric reflux. Eur. J. Pediatr., 140: 212, 1983.
  7. Handmaker, H., Young, B. W. and Lowenstein, J. M.: Clinical experience with 99 mTc-DMSA (dimercaptosuccinic acid), a new renal-imaging agent. J. Nucl. Med., 16: 28, 1975.
  8. McAfee, J. G .: A review of radiopharmaceuticals in nephrourology. In: Radionuclides in Nephrology. Edited by A. M. Joekes, A. R. Constable, N. J. G. Brown and W. N. Tauxe. London, Academic Press, s.l. pp. 3-9, 1982.
  9. Taylor, A., Jr.: Quantitation of renal function with static imaging agents. Semin. Nucl. Med., 12: 330, 1982.
  10. Taylor, A. Jr., Lallone, R. L. and Hagan, P. L.: Optimal handling of dimercaptosuccinic acid for quantitative renal scanning. J. Nucl. Med., 21: 1190, 1980.
  11. Raynaud, C., Ricard, S. and Knipper, M.: Etude du taux de la fixation renale du 99 mTc-DMSA. In: La Fixation Renale due Hg (1 97 HgCb) et du DMSA ( 99 mTc-DMSA). Comptes Rendus du Ileme Collogue International sur la Fixation Renale due Hg Radioactif. Edited by C. Raynaud. Paris, pp 233-245, 1975.
  12. Yee, C. A., Lee, H.B. and Blaufox, M. D.: Tc-99m DMSA renal uptake; influence of biochemical and physiologic factors. J. Nucl. Med., 22: 1054, 1981.
  13. Bowman, R. H. and Maack, T.: Glucose transport by the isolated perfused rat kidney. Am. J. Physiol., 222: 1499, 1972.
  14. Fray, J. C. S.: Stimulation of renin release in perfused kidney by low calcium and high magnesium. Am. J. Physiol., 232: 377, 1977.
  15. Nishiitsutsuji-Uwo, J.M., Ross, B. D. and Krebs, H. A.: Metabolic activities of the isolated perfused rat kidney. Biochem. J., 103: 852, 1967.
  16. Sigulem, D., Saito, H., Ajzen, H. and Ramos, 0. L.: The influence of magnesium on renin secretion by the artificially perfused rat kidney. In: Magnesium in Health and Disease. Proceedings of the 2nd International Symposium on Magnesium, Montreal, Oxford Press, p 79-82, 1976.
  17. Krebs, H. A. and Henseleit, K.: Untersichungen uber die harn- stoffbildung in tierkorper. Z. Physiol. Chem., 210: 33, 1932.
  18. Henry, R. J., Cannon, D. C. and Winkelman, J. W.: Nonprotein nitrogenous constituents. In: Clinical Chemistry Principles and Technics. 2nd ed. New York, Harper and Row, c. 17, pp. 543- 552, 1974.
  19. O'Mara, R. E., Capps, S. J. and Hall, J. N.: Changes in the distribution of short-lived radionuclides in the kidney: studies by autoradiography. J. Nucl. Med., 16: 554, 1975.
  20. Irish, J. M. III. and Grantham, J. J.: Renal handling of organic anions and cations. In: The Kidney. 2nd Ed. Edited by B. M. Brenner and F. C. Reactor, Jr. Philadelphia, W. B. Saunders, V. 1, C. 13,pp. 619-649, 1981.
  21. Maack, T., Johnson, V., Kan, S. T., Figueiredo, F. J. and Sigulem, D.: Renal filtration, transport and metabolism of low-molecular weight proteins: a review. Kidney Int., 16: 251, 1979.
  22. Willis, K. W., Martinez, D. A., Hedley-Whyte, E. T., Davis, M. A., Judy, P. F. and Treves, S.: Renal localization of 99 mTc-stannous glucoheptonate and 99 mTc-stannous dimercaptosuccinate in the rat by frozen section autoradiography. Radio!. Res., 69: 475, 1977.
  23. Moretti, J. L., Rapin, J. R., Le Poncin, M., Lageron, A., Saccavini, J. C. and Bardy, A.: Dimercapto-succinic acid complexes: their structure, biological behaviour and renal localization. In: Radio- nuclides in Nephrology. Edited by A. M. Joekes, A. R. Constable, N. J. G. Brown and W. N. Tauxe, London, Academic Press, s.l pp. 25-31, 1982.
  24. Vanlic-Razumenic, N. and Petrovic, J.: Biochemical studies of the renal radiopharmaceutical compound dimercaptosuccinate. II. Subcellular localization of 99 mTc-DMS complex in the rat kidney in vivo. Eur. J. Nucl. Med., 7: 304, 1982.