Description of Gluconacetobacter sacchari sp. nov., a new species of acetic acid bacterium isolated from the leaf sheath of sugar cane and from the pink sugar-cane mealy bug (original) (raw)

1999, International Journal of Systematic and Evolutionary Microbiology

Bureau of Sugar A new species of the genus Gluconacefobacfer, for which the name Gluconacefobacfer sacchari sp. nov. is proposed, was isolated from the leaf sheath of sugar cane and from the pink sugar-cane mealy bug, Saccharicoccus sacchari, found on sugar cane growing in Queensland and northern New South Wales, Australia. The nearest phylogenetic relatives in the a-subclass of the Pro f eobacferia a re Glucona ce f obacter lique faciens and Gluconace tobacf er diazotrophicus, which have 988-993 O/ O and 97.9-98-5 O/ O 165 rDNA sequence similarity, respectively, to members of Gluconacetobacfer sacchari. On the basis of the phylogenetic positioning of the strains, DNA reassociation studies, phenotypic tests and the presence of the Q l O ubiquinone, this new species was assigned to the genus Gluconacefobacfer. No single phenotypic characteristic is unique to the species, but the species can be differentiated phenotypically from closely related members of the acetic acid bacteria by growth in the presence of 0 0 1 YO malachite green, growth on 30% glucose, an inability to f i x nitrogen and an inability to grow with the L-amino acids asparagine, glycine, glutamine, threonine and tryptophan when D-mannitol was supplied as the sole carbon and energy source. The type strain of this species is strain SRI 1794T (= DSM 127173.