Puerarin 6″‐O‐xyloside suppresses growth, self‐renewal and invasion of lung cancer stem‐like cells derived from A549 cells via regulating Akt/c‐Myc signalling (original) (raw)

Clinical and Experimental Pharmacology and Physiology, 2020

Abstract

Cancer stem cells have been identified as the major cause of cancer initiation and progression. To investigate the effects of puerarin 6″‐O‐xyloside (PXY), derived from Pueraria lobata (Willd.) Ohwi, on lung cancer stem cells, we enriched and identified a subpopulation of lung cancer stem‐like cells (LCSLCs) derived from lung adenocarcinoma A549 cells with traits including high self‐renewal and invasive capability in vitro, elevated tumourigenicity in vivo, and high expression of stem cell markers CD44, CD133 and aldehyde dehydrogenase 1 (ALDH1). We found that PXY could impair cell viability, suppress self‐renewal and invasive capability, and decrease CD133, CD44 and ALDH1 mRNA expression in LCSLCs in a dose‐dependent manner. Furthermore, we showed that PXY suppressed the self‐renewal and invasive capability of LCSLCs at least in part through suppressing the activation of Akt/c‐Myc signalling. In conclusion, PXY can block the traits of LCSLCs, indicating that PXY may be a candidate compound for lung adenocarcinoma therapy via eliminating LCSLCs.

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