Identification and Molecular Characterization of Alpha Papillomavirus from Male Olive Baboons (Papio anubis) Maintained in a Captive Colony (original) (raw)

American Journal of Infectious Diseases and Microbiology, 2018

Abstract

Papillomaviruses (PVs) are ubiquitous highly diverse group of circular double stranded DNA viruses. Nearly all the human papillomavirus (HPVs) that cause cancer are clustered in Alphapapillomavirus (αPV) genera and have a common ancestor. The aim of the study was to isolate and perform molecular characterization of alphapapilloma virus from male olive baboons (Papio anubis) that are maintained in a captive colony at the Institute of Primate Research, Nairobi, Kenya and establish their evolutionary relationship with known strains responsible for various species causing cervical cancer in human. Twenty (n=20) different genital swabs from sexually active male olive baboons were collected. Positive samples for αPV by nested PCR were 9/20 (45%). The nested PCR primers targeted a conserved region of L1 major capsid gene and aided in generating amplicons of 134bp. Only three amplicons with good quality bands (1C, 2C, and 4C) were further sequenced and analysed using MEGA X, Clustal W algor...

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