Use of Lactobacillus johnsonii in broilers challenged with Salmonella sofia (original) (raw)
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The present experiment examined the influence of Bacillus subtilis PB6 and Bacillus cereus var. toyoi based probiotics, as an alternative to in-feed antibiotic, neoxyval, on broilers' performance during pre-and post-challenge with Salmonella enterica subsp. typhimurium. Ten cages of birds received one of the following treatments: T1 = positive control, unmedicated, unchallenged (+CONT); T2 = negative control, unmedicated, challenged (-CONT); T3 = medicated with antibiotic (neoxyval), challenged (NEOX); T4 = medicated with probiotic Toyocerin ® , challenged (TOYO); and T5 = medicated with probiotic CloSTAT TM , challenged (CLOS). Chicks in treatments T2 to T5 were challenged with (3 × 10 9 CFU/ml) of Salmonella enterica subsp. typhimurium on day 16. The results revealed that unchallenged bird (CONT) consumed more feed as compared to other treatments (P < 0.001). Furthermore, birds, which had received NEOX, CLOS and TOYO converted feed more efficiently as compared to those whic...
Effect of a Multi-Species Probiotic on the Colonisation of Salmonella in Broilers
Probiotics and Antimicrobial Proteins, 2019
Newly hatched broiler chickens are the most susceptible to Salmonella infections, especially during the first 24 h. At this age, the gut microbiome is not fully developed and offers little protection in the form of competitive exclusion. In this study, one group of newly hatched, Salmonella-free broilers were colonised with a multi-species probiotic (2.0 × 10 10 to 8.9 × 10 10 CFU per kg feed) for 28 days, consisting of Lactobacillus crispatus, Lactobacillus salivarius, Lactobacillus gallinarum, Lactobacillus johnsonii, Enterococcus faecalis and Bacillus amyloliquefaciens. Broilers in another group received oxytetracycline (200 mg/kg feed), instead of the probiotic, for 28 days. On days 9 and 10, broilers in both groups were gavaged with 9 × 10 7 CFU Salmonella Enteritidis A9, a pathogenic strain isolated from infected broilers. On day 14, Salmonella was detected in the ceca of 95% of broilers treated with the multi-species probiotic, but 2 weeks later, almost half of the birds (45%) had no Salmonella in their ceca. Similar results were recorded after 28 days of treatment with oxytetracycline. Only 10% of Salmonella-infected birds not treated were Salmonella-free on day 28. Growth performance, immune organ weight (spleen and bursa of Fabricius) and whole blood cell counts of birds treated with the multispecies probiotic and oxytetracycline, respectively, were similar to untreated and uninfected birds throughout the 28-day trial (p > 0.05). On day 14, serum lysozyme levels of broilers exposed to the probiotic were lower (8.0 μg/mL) compared with those of broilers treated with oxytetracycline (11.0 μg/mL). Although the multi-species probiotic and oxytetracycline stimulated the immune system, probiotics are safer to use than antibiotics and should be the preferred choice of treatment.
2016
This experiment was conducted to compare the effects of multi-strain probiotic inclusion levels on performance, intestinal morphology, gut microflora and hematological changes in Salmonella typhimurium (ST) infected broiler chickens. A total of 120 day old Ross 308 broiler chickens were distributed into 20 floor pens and reared for 10 days under 5 experimental treatments including a corn-soy basal diet with no probiotic (Control), or 0.5 g (0.05%), 1 g (0.1%), 1.5 g (0.15%) and 2 g (0.2%) probiotic/kg diet. Chickens were infected orally with ST at second day of experiment. Broilers under 0.15% of probiotic had higher body weight gain compared to other treatments. Probiotic supplementation, except at 0.05%, significantly improved feed conversion ratio. The use of 0.1 and 0.15% of probiotic reduced the population of Salmonella in ileum. The lowest heterophil:lymphocyte ratio was observed in 0.15% of probiotic, although 0.1 and 0.2% of probiotic significantly reduced this ratio compare...
Journal of Animal and Poultry Production, 2009
To study the effect of Probiotics and lactose on the Salmonella colonization and immunity in Inshas and Matrouh local broilers purebreds and crossbreds, ten groups of broilers chicks were categorized and offered different treatments of probiotics including Lactobacillus acidophilus, Bacillus subtilis and Enterococcus faeceium alone or accompanied by 2.5% Lactose in drinking water. Different parameters were evaluated including body weight, feed conversion, feed intake, daily gain, livability, caecal Salmonella count, caecal pH and antibody titer against Salmonella. Results showed that, Enterococcus faeceium had significant effects on body weight and daily gain while none of the used treatments had significant effects on livability of the examined chicks. Enterococcus faeceium and Bacillus subtilis had significant effects on feed intake only at 7 days of age while Bacillus subtilis showed a significant difference on feed conversion only at 28 days age. Inshas x Matrouh crossbred proved to be the most effective in reducing Salmonella count at 28 days. All treatments caused reduction of caecal pH and Lactobacillus acidophilus with lactose 2.5% had the highest effect. Matrouh x Inshas crossbred showed the strongest immunity reaction against Salmonella if compared with the other breeds. Enterococcus faeceium together with lactose gave also the strongest immune reaction against Salmonella if compared with the other breeds.
Journal of Veterinary Science and Animal Husbandry
Performance and productivity in the poultry industry lately is predicated on the use of antimicrobials, which has led to various negative impacts; among which include the emergence of a variety of pathogens and bacterial resistance including salmonella sp. This study was designed to investigate periodic evaluation of the protective properties of probiotic on commercial broilers experimentally infected with Salmonella Enteritidis; specifically on bacterial shedding and mortality pattern of birds throughout the rearing period. One hundred and eighty day old commercial broilers were allotted into four treatment groups, G1 (antibiotics, probiotic & S. Enteritidis infected), G2 (antibiotics & S. Enteritidis infected), G3 (probiotic & S. Enteritidis infected) and G4 (antibiotics) each replicated thrice, with 15 birds per replicate. Antibiotic and probiotics were administered day 1-5 and 6-56 days respectively; thereafter, cultured Salmonella Enteritidis was inoculated at the dose rate of 107cfu orally. This study lasted for eight (8) weeks, during which freshly voided faecal samples were collected from the respective replicates for isolation, biochemical test and salmonellae counts; for pre-infection (0), 7, 10, 15, 18 and 21 day post infection (dpi). Data generated were subjected to descriptive statistical analysis and significant differences (p<0.05) between mean values determined with student t-test. The numbers of dead birds over a period of 8weeks were calculated and expressed as percentage of allotted birds in each treatment group.
Poultry Science, 2008
We evaluated the effect of a Lactobacillusbased probiotic culture (FM-B11) for reduction of Salmonella Enteritidis in neonatal broiler chicks. In all experiments, chicks were challenged with approximately 10 4 cfu of Salmonella Enteritidis upon arrival at our laboratory, and the treatments were administered 1 h postchallenge. Cecal tonsil samples were obtained 24 h posttreatment and enriched for Salmonella Enteritidis recovery. The first experiment compared the effects of oral administration of doses of 10 4 , 10 6 , and 10 8 cfu/chick. In this experiment, doses of 10 6 and 10 8 both significantly reduced Salmonella Enteritidis recovery compared with controls (15 vs. 85% Salmonella Enteritidis positive), but 10 4 cfu did not significantly reduce Salmonella Enteritidis recovery. The second experiment compared the efficacy of oral administra
Approaches in Poultry, Dairy & Veterinary Sciences
The objective of the present study was to evaluate the effect of a lactic acid based probiotic on leaky gut and microbiome associated challenges with Salmonella Enteritidis infection and 24h feed restriction (FR) in broiler chickens. Chickens were orally gavaged with 2x10 4 cfu/chick of S. Enteritidis at 1 d of age and then were randomly assigned to one of four groups: 1) Control, 2) Probiotic control, 3) FR, and 4) Probiotic+FR. The probiotic was included in the drinking water for 16 days. Blood samples were collected for measuring leakage of FITC-d, and ceca content was also collected for microbiome evaluation. In the present study, the probiotic reduced FITC-d when compared with FR chickens without the probiotic. At Phylum level, both groups treated with probiotic had higher proportion of Firmicutes and Bacteroidetes. At the Class level, both control groups in this trial had an increase in Gammaproteobacteria. This study confirms that FR increases gut permeability in chickens, but these changes were prevented by the administration of a lactic acid based probiotic.
Avian Pathology, 2016
Two experiments were conducted to evaluate the effect of a lactic acid bacteria-based probiotic (FloraMax-B11 ®) against Salmonella enterica serovar Enteritidis intestinal colonization and intestinal permeability in broiler chickens. Experiment 1 consisted of two independent trials. In each trial, day-old broiler chicks were assigned to one of two groups: control + S. Enteritidis or probiotic + S. Enteritidis. At 72 h postS. Enteritidis challenge, haematology and caecal content were evaluated for S. Enteritidis colonization. In Experiment 2, day-old broiler chicks were assigned to one of four groups: negative control; probiotic; control + S. Enteritidis; or probiotic + S. Enteritidis. At 72 h postS. Enteritidis challenge, chickens in all groups were given an oral gavage dose of fluorescein isothiocyanate dextran (FITC-d). In both trials of Experiment 1, a significant reduction (P < 0.05) in colony-forming units/gram of S. Enteritidis in caecal content and a reduction in the incidence of S. Enteritidis enriched caecal samples were observed in probiotic + S. Enteritidis chickens. In addition, significant heterophilia and lymphopaenia were observed in control + S. Enteritidis chickens. In Experiment 2, a decrease in numbers of S. Enteritidis in caeca were observed in probiotic + S. Enteritidis chickens when compared to control + S. Enteritidis. Also, an increase in serum FITC-d concentration was detected in control + S. Enteritidis. These results suggest that early infection with S. Enteritidis can increase intestinal permeability, but the adverse effects can be prevented by the administration of the probiotic tested.
The Effect of a Lactobacillus-Based Probiotic for the Control of Necrotic Enteritis in Broilers
Food and Nutrition Sciences, 2013
Necrotic Enteritis (NE) caused by Clostridium perfringens (CP) in poultry is probably the most important bacterial disease in terms of economic implications. The disease is multi-factorial and is invariably associated with predisposing factors. The present study investigated the effect of a commercially available Lactobacillus-based probiotic (FM-B11) for the control of necrotic enteritis in broiler chickens. In experiment 1, one-day-of-hatch broiler chicks were randomly allocated to the following treatment groups: 1) Non-challenged (NC); 2) Challenged (C); 3) Challenged + probiotic (C+ FM-B11). Prior to placement, chicks in groups 2 and 3 received 0.25 mL of Salmonella typhimurium (ST) containing 10 5 cfu of viable cells by oral gavage. At 14, 15 and 16 days of age, all chicks in group 3 were treated with FM-B11 in the drinking water at a concentration of 10 6 cfu/ml. At 21d of age, all chicks in groups 2 and 3, were individually challenged with 5 × 10 4 sporulated oocysts of E. maxima by oral gavage. At 26d of age, all chicks in groups 2 and 3, were individually challenged with 10 8 cfu CP; body weight (BW) was recorded prior to challenge. The experiment was terminated at 29 days of age and the following parameters were evaluated: NE-associated mortality, CP lesion scores, CP concentrations in ileum, BW, and body weight gain (BWG). Chicks treated with FM-B11 had significantly (P < 0.05) higher body weight gain after challenge when compared to control challenge chickens. Total mortality was higher in the C group (48.8%) when compared to the C + FM-B11 (12.7%). Even though there was no significant (P > 0.05) difference in lesion score between C and C + FM-B11, group C + FM-B11 had significantly (P < 0.05) lower total number of cfu of CP recovered from the ileal mucosa and content samples when compared to group C. Experiment 2 was a unique and remarkable case report of a field outbreak of NE in a commercial broiler farm in Argentina. A reduction and control of the mortality associated with NE following 3 days of administration of FM-B11 was observed as compared with the control non treated house. These results imply that the commercially available Lactobacillus-based probiotic FM-B11 was able to reduce the severities of NE, as a secondary bacterial infection, in an experimental NE challenge model; as well as, in a commercial field outbreak of NE.
Food and Nutrition Sciences, 2013
Necrotic Enteritis (NE) caused by Clostridium perfringens (CP) in poultry is probably the most important bacterial disease in terms of economic implications. The disease is multi-factorial and is invariably associated with predisposing factors. The present study investigated the effect of a commercially available Lactobacillus-based probiotic (FM-B11) for the control of necrotic enteritis in broiler chickens. In experiment 1, one-day-of-hatch broiler chicks were randomly allocated to the following treatment groups: 1) Non-challenged (NC); 2) Challenged (C); 3) Challenged + probiotic (C+ FM-B11). Prior to placement, chicks in groups 2 and 3 received 0.25 mL of Salmonella typhimurium (ST) containing 10 5 cfu of viable cells by oral gavage. At 14, 15 and 16 days of age, all chicks in group 3 were treated with FM-B11 in the drinking water at a concentration of 10 6 cfu/ml. At 21d of age, all chicks in groups 2 and 3, were individually challenged with 5 × 10 4 sporulated oocysts of E. maxima by oral gavage. At 26d of age, all chicks in groups 2 and 3, were individually challenged with 10 8 cfu CP; body weight (BW) was recorded prior to challenge. The experiment was terminated at 29 days of age and the following parameters were evaluated: NE-associated mortality, CP lesion scores, CP concentrations in ileum, BW, and body weight gain (BWG). Chicks treated with FM-B11 had significantly (P < 0.05) higher body weight gain after challenge when compared to control challenge chickens. Total mortality was higher in the C group (48.8%) when compared to the C + FM-B11 (12.7%). Even though there was no significant (P > 0.05) difference in lesion score between C and C + FM-B11, group C + FM-B11 had significantly (P < 0.05) lower total number of cfu of CP recovered from the ileal mucosa and content samples when compared to group C. Experiment 2 was a unique and remarkable case report of a field outbreak of NE in a commercial broiler farm in Argentina. A reduction and control of the mortality associated with NE following 3 days of administration of FM-B11 was observed as compared with the control non treated house. These results imply that the commercially available Lactobacillus-based probiotic FM-B11 was able to reduce the severities of NE, as a secondary bacterial infection, in an experimental NE challenge model; as well as, in a commercial field outbreak of NE.