Pattern of dermatophytes among skin, hair, and nail specimens in a tertiary care hospital of Lahore (original) (raw)
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Trichophyton rubrum the predominant etiological agent in human dermatophytoses in Chennai, India
African Journal of Microbiology Research, 2007
The present study was undertaken to find the predominant etiological agent of dermatophytoses among the patients attending the out patient clinic of Mycology Section, Department of Dermatology, Madras Medical College and Hospital, Chennai. Specimens were collected from suspected patients with dermatophytoses and examined for the presence of fungal elements, cultured, isolated and identified. Among the 90 suspected patients with clinical symptoms of dermatophytoses, 71 (78.9%) were confirmed in culture. Trichophyton genus was accounted for 93% of dermatophytoses, which was shared by Trichophyton rubrum (73.3%) andTrichophyton mentagrophytes (19.7%), followed by Epidermophyton floccosum (4.2%) and Microsporum gypseum (2.8%). It was noted that tinea corporis (64.8%) is the most prevalent infection followed by tinea cruris (26.8%), tinea pedis (5.6%) and onychomycoses (2.8%). T. rubrum was the predominant species responsible for the dermatophytoses, especially tinea corporis in Chennai,...
Clinico-Mycological Profile of Dermatophytoses at a Tertiary Care Teaching Hospital of Central India
SVU-International Journal of Medical Sciences
Background: Dermatophytosis is a disease of hair, nails, and stratum corneum of the skin caused by dermatophytes. The prevalence of dermatophytosis in a geographical area depends on a variety of factors such as climate, personal hygiene, and individual susceptibility. The clinical importance of isolating and identifying dermatophytes is to start appropriate treatment & to detect probable infection sources. Also, identification is important for prognostic consideration. Objectives: Our study aims to know the clinico-mycological profile in suspected cases of dermatophytosis. Patients and Methods: A total of 110 suspected cases of dermatophytoses that were diagnosed clinically by a dermatologist were included in this study. Specimen of skin scrapings, hairs & nail clippings wherever appropriate were collected from these patients. Specimens collected were subjected to standard mycological procedures. Results: In our study, the most common age group affected was 21-30 years (31.82%). The majority of the cases were from the lower middle class (38%). The commonest clinical type was Tinea corporis (48%). In 72.73% of cases, we were able to detect fungi either by direct microscopy and/or culture. Out of 62 culture isolates, T.rubrum was found to be the commonest (59.7%), followed by T.mentagrophytes (24.2%), E.floccosum (6.5%), T.tonsurans (3.2%), M. gypseum (3.2%) and one isolate each of M. audouinii and M. canis. Conclusion: With proper techniques, various species of dermatophytes can be identified. But conventional methods are time-consuming and a week to a month is required for identification to species level. So the development of rapid molecular techniques is the need of the hour.
Trichophyton rubrum: The Commonest Isolate from Dermatophytosis
2012
Dermatophytosis is a trivial disease but has lot of psychological effect and a costly disease in terms of treatment. Though various species of dermatophytes produce clinically characteristic lesions, but a single species may produce variety of lesions depending upon site of infection. The present study was undertaken with following aim and objectives. Isolation and identification of different etiological agents causing dermatophytosis. Correlation between the site of involvement and the causative agents.
Fungal strains isolated from several cases of human dermatophytoses
Scientific Bulletin. Series F. Biotechnologies, 2013
Dermatophytosis has become one of the most common human infectious diseases in the world so it is of interest to dedicate more studies to its etiological agent's dermatophytes. These keratinophilic and keratinolytic filamentous fungi have the ability to invade and colonize keratinized layers of the skin and their appendages. Dermatophytes fungi group include three anamorphic genera namely Epidermophyton, Microsporum and Trichophyton. These three genera include geophilic, zoophilic and anthropophilic species. Usually these filamentous fungi are identified on the basis of conidia morphology and sometimes with specific physiological characters, such as the hair strand perforation and urea hydrolysis. The objective of present study was to isolate and to identify some filamentous dermatophytes fungi from human superficial mycoses. Isolated samples (scales, fragments of nails and subungual debris) were cultured on specific culture media during four months. After incubation time, morphological characters of cultured fungal were observed macroscopically and microscopically. The following cultural characteristics were analyzed: texture, surface and reverse color of the colony, the presence of pigmentation. Microscopic examination offered data on specific characters such as, the presence/absence of macroconidia and microconidia, theirs shape, their septa number, the presence of chlamydoconidia, spiral hyphae and nodular organs. The strains identification was completed with in vitro hair strand perforation and urea hydrolysis. The isolated microbial strains were identified as belonging to Trichophyton and Microsporum genera.
Current trends of Clinicomycological Profile of Dermatophytosis in Central India
Abstract: Background: Dermatophytosis is one of the most common cutaneous fungal infections of public health importance. Its prevalence differs from place to place and is influenced by environmental conditions, personal hygiene and habits. Aim: The present study was undertaken to assess the clinical and mycological profile of dermatophytic infection and identify the species of fungi using standard techniques. Materials and methods: A cross sectional study was conducted in 100 clinically diagnosed patients of dermatophytosis attending the dermatology outpatient department of our hospital. Proforma containing structured questionnaire was also filled. Skin scrapings, nail scrapes or nail clippings and infected hair stubs were collected. All specimens were screened for dermatophytes by direct microscopy using KOH DMSO preparation and confirmed by fungal culture. Results: Tinea unguium (52.0%) was predominant clinical condition. Males were affected more (79.0%) than females. Dermatophytosis was predominantly found in more than 60 years (32.0%) and 31-45 years (24.0%). Fungi were demonstrated in 55.0% cases by KOH mount and 46.0% cases were positive by culture. 16.0% cases were KOH negative and culture positive. Trichophyton rubrum (41.0%) was the predominant species. Conclusions: Males with age group above 60 years were most commonly affected in our area. Predominant clinical type was tinea unguium probably because most of them were farmers and labourers with poor hygiene. Trichophyton rubrum was the commonest dermatophyte isolated. KOH negative and culture positive cases indicate that culture is a gold standard for isolation and identification of dermatophytes. Key words: Dermatophyte, Dermatophytosis, Tinea, Trichophyton
Background: Dermatophyte, a keratinophilic fungus which can invade keratinized tissue cause dermatophytosis. It is one of the major superficial fungal infections. According to World Health Organization (WHO), 20-25% world population is affected by dermatophytes. The aim of this study was to identify different species of dermatophytes causing dermatophytosis at a tertiary care hospital in Bangladesh. Methods: Total 246 skin, nail and hair samples were collected from Dermatology and Venereology department of Dhaka Medical College Hospital (DMCH), Dhaka, Bangladesh. Dermatophytes were identified by microscopy, culture, biochemical test (urease test and hair perforation test) and polymerase chain reaction (PCR). Fungal elements were observed under microscope. Sabouraud Dextrose Agar (SDA) and Dermatophyte Test Medium (DTM) were used to culture the specimen. Species identification was confirmed by biochemical test and PCR. Results: Among 246 clinically diagnosed cases of dermatophytosis, 91 (36.98%) cases were observed among the age group of 21-30 years. Female were more affected than male (male-female ratio was1:1.34). Eighty-five (34.55%) cases were positive by microscopy and 76 (30.89%) cases were positive in culture. Trichophyton mentagrophyte was the common etiological dermatophyte species which was 59.21% followed by Trichophyton rubrum (40.79%). All culture isolates were further evaluated by biochemical test and PCR. Most of the tinea corporis was caused by Trichophyton mentagrophyte which was 64.81%. Conclusion: Dermatophytosis is a common infection in young adults specially females. Trichophyton mentagrophyte is more frequent type of dermatophyte. Majority of dermatophytes could be identified by both direct microscopy and culture methods.
Current Medical Mycology, 2021
Background and Purpose: The most common etiological agents of human dermatophytosis in various parts of the world are Trichophyton rubrum, Trichophyton interdigitale, and Epidermophyton floccosum. The main aim of this study was to design and evaluate a simple and straightforward multiplex polymerase chain reaction (PCR)assay for reliable identification/differentiation of these species in clinical isolates. Materials and Methods: The reliable sequences of several molecular targets of dermatophytes species were used to design a multiplex PCR for the identification of common pathogenic dermatophytes. The isolates and clinical specimens examined in this study included seven standard strains of dermatophytes, 101 isolates of dermatophytes and non-dermatophyte molds/yeasts which had already been identified by sequencing or PCR-restriction fragment length polymorphism (RFLP), and 155 clinical samples from patients suspected of cutaneous mycoses. Results: Species-specific primer pairs for T...
Prevalence and aetiology of dermatophytoses in Isfahan, Iran
Mycoses, 1997
In this study the prevalence and causative agents of dermatophytoses in Isfahan, a large province of Iran, were determined. Of 16 578 clinically suspected cases 13.3% were affected with dermatophytoses. Lesions of tinea capitis were the most prevalent clinical type of dermatophytoses (54.1 %), followed by tinea corporis (23.8%) and tinea pedis (8.9%). Trichophyton uerrucosum was the most frequent causative agent (32.8%), followed by Epidemophytonjoccosum (1 7.6%), T. mentagrophytes (16.2%) and Microsporum canis (12.3%). We found a relationship between the spread of dermatophytoses and livestock infected with dermatophytoses in Isfahan. Zusammenfassung. Pravalenz und Erregerspektrum von Dermatophytosen wurden in Isfahan untersucht, einer groBen Provinz in Iran. Von 16 578 klinischen Verdachtsfdlen waren 13,3% mit Dermatophytosen behaftet. Davon waren 54,l O/o Tinea capitis, 23,8% Tinea corporis und 8,9% Tinea pedis. Der haufigste Erreger war Trichophyton verrucosum (32,8%), gefolgt von Epidermophyton floccosum (17,6), 1. mentagropytes (16,2%) und Microsporum canis (1 2,3%). Zusammenhange zwischen der Dermatophytosen-Verbreitung und Dermatophytosen im Viehbestand konnten aufgezeigt werden.
Mycological Pattern of Dermatophytes and Non- Dermatophytes in a Tertiary Care Hospital
https://www.ijhsr.org/IJHSR\_Vol.10\_Issue.4\_April2020/IJHSR\_Abstract.05.html, 2020
Background: Dermatophytosis is currently a disease of worldwide importance and a public health problem in many parts of the world particularly in developing countries. Objective: This study was carried out to determine the pattern of dermatophytes in a tertiary care hospital from July 2015 to April 2019. Methods: Nail, skin, and scalp scrapings were received from 657 patients and were used for microscopy and culture study. Fungal pathogens were identified by studying the macroscopic and microscopic characteristics of their colonies. Results: Amongst 657 clinical samples received, 280 (42.62%) were found to be positive microbiologically. Out of 280 positive samples, 189 (28.76%) were KOH positive while 91 (13.85%) were culture positive. Amongst 91 fungal isolates obtained on culture, 53 were dermatophytes and 38 were non-dermatophyte fungi. Trichophyton mentagrophytes represented 60.38% of dermatophyte isolates. Conclusions: Coupling of clinical diagnosis with laboratory diagnosis appeared to be essential for better diagnosis as the cost and long duration of fungal therapy underline the significance of accurate diagnosis of the condition before starting therapy.