Crystallographic Investigation of A-DNA Conformations: The Effect of Hydration, Methylation and Spermine Binding / (original) (raw)

1995

Abstract

Hydration, methylation and spermine binding drastically affect the conformation and behavior of duplex DNA oligomers. Ciystallographic analysis of eight DNA octamers that crystallize isomorphously as A DNA in the tetragonal space group P43212, reveals a strong dependence of their major groove hydration on local helix parameters. Increased states of hydration are correlated with high twist angles, negative base rolls, and GC base pairs. Local helix geometry also has qualitative effects on the hydration patterns in the major grooves of the tetragonal A DNA octamers. A second regulator of DNA conformation is spermine, which has been found in the crystal lattices of the tightly packed A DNA decamers d(CCGGGCCCGG) and d(CCGGGCCMcCGG). Ordered spermines are located in regions of high phosphate density and, consequently, facilitate the condensation, bending and twisting of duplex DNA. The methylated derivative has been crystallized in three different conformational states and two crystal s...

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