Evaluating the use of Lactobacillus acidophilus as a biocontrol agent against common pathogenic bacteria and the effects on the haematology parameters and … (original) (raw)

This study was carried out to evaluate the use of Lactobacillus acidophilus as a biocontrol agent against some common ¢sh pathogenic bacteria (Staphylococcus xylosus, Aeromonas hydrophila gr.2 and Streptococcus agalactiae) in African cat¢sh, Clarias gariepinus. Eight treatments were designed inclusive of 10 C. gariepinus juveniles (mean weight 190 g) per tank, each in triplicate. Four groups of ¢sh were fed a diet supplemented with L. acidophilus, comprising about 3.01 Â 10 7 colony-forming units per gram of diet (the probiotics diet), while the other four groups were fed a diet not supplemented with probiotics (the non-probiotics diet). In the ¢rst group, ¢sh were injected with 1mL physiological saline and fed the non-probiotic diet (non-probiotic control); in the second, third and fourth groups, ¢sh were injected with 1mL each of S. xylosus, A. hydrophila gr.2 and S. agalactiae, respectively, and were all fed the non-probiotic diet (designated as non-probiotic treatments; NPsx, NPah and NPsa respectively). In the ¢fth group, ¢sh were injected with 1mL physiological saline but fed the probiotic diet (probiotic control), while ¢sh in the sixth, seventh and eighth groups were each injected with 1mL of S. xylosus, A. hydrophila gr.2 and S. agalactiae, respectively, and were all fed the probiotic diet (and designated as probiotic treatments; Psx, Pah and Psa respectively). Blood samples were collected for haematology analysis, while samples of the liver and kidney were examined for pathohistology after 7 and 21 days of infection. The results showed that the haematology parameters, packed cell volume, haemoglobin, erythrocyte sedimentation rate, red blood cell, white blood cell, total serum protein, Mg 21 , Ca 21 , Cl À , glucose, cholesterol and total immunoglobulin concentrations and the pathohistology of the liver and kidney were better in the challenged ¢sh (infected) maintained on the probiotic diet than those in the groups fed the non-probiotic diet. It is concluded, based on these results, that L. acidophilus is useful as a probiotic agent in C. gariepinus against these pathogenic bacteria (S. xylosus, A. hydrophila gr.2 and S. agalactiae). , 42, 196^209 L. acidophilus as a probiotic in African cat¢sh M A Al-Dohail et al.