Photo induced mechanistic activity of GO/Zn(Cu)O nanocomposite against infectious pathogens: Potential application in wound healing (original) (raw)

Photodiagnosis and Photodynamic Therapy, 2021

Abstract

Treating infection causing microorganisms is one of the major challenges in wound healing. They gain resistance due to the overuse of conventional antibiotics. Biocompatible nanomaterials with enhanced antibacterial activity are developed to overcome the bacterial resistance. Another promising technique called antimicrobial photodynamic therapy (aPDT) was used to selectively to cause damage infectious pathogenic cells via more generation of reactive oxygen species (ROS). GO/Zn(Cu)O nanocomposite was synthesized by co-precipitation method. Graphene Oxide (GO) is known for its high surface to volume ratio, excellent surface functionality and enhanced antimicrobial property. ZnO nanoparticle induces the generation of reactive oxygen species (ROS) under light irradiation and it leads to recombination of electron-hole pair. Nanocomposites of GO and Cu doped ZnO increases visible light absorption and enhances the photocatalytic property. It generates more ROS and increases the bacterial inhibition. GO/Zn(Cu)O nanocomposite is tested against Staphylococcus aureus (S. aureus), Enterococcus faecium (E. faecium), Escherichia coli (E. coli), Salmonella typhi (S. typhi), Shigella flexneri (S. flexneri) and Pseudomonas aeruginosa (P. aeruginosa) by well diffusion method, growth curve, colony count, biofilm formation under both dark and visible light condition. Reactive Oxygen Species assay (ROS), Lactate dehydrogenase leakage (LDH) assay, Protein estimation assay and membrane integrity study proves the mechanism of inhibition of bacteria. Inhibition kinetics shows the sensitivity between bacteria and GO/Zn(Cu)O nanocomposite.

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