Assessment of Phytochemical Analysis, Nutritional Composition and Antimicrobial Activity of Moringa oleifera (original) (raw)

Phytochemical and Antibacterial Activity of Moringa Oleifera Available in the Market of Mekelle

The aim of the present study was to evaluate the antibacterial activity of Moringa oleifera leaf extracts, Escherichia coli, Pseudomonas aeroginosa, Staphylococcus aureus, Proteus vulgaris, Streptococcus mutans, Bacillus subtilus, and Staphylococcus epidermidis bacteria were used. Phytochemical analyses of the leaf in solvents of varying polarity with aqueous, ethanol were also carried out. The phytochemical screening indicated the presence of flavonoids, tannins, steroid, alkaloid, saponins etc., in the both extracts. Well diffusion method was used to assess the antibacterial effect of the extracts on micro-organisms. The ethanolic and aqueous extract were active against all strains but the ethanol leaf extract showed maximum activity against Streptococcus mutant and aqueous extract shows maximum activity against Proteus vulgaris. The research showed that the higher reducing power of the aqueous extract could be due to the better solubility of the antioxidant components in water whereas the predominant antibacterial activity in organic solvent extracts as compared to aqueous extracts, indicates that the active components responsible for the bactericidal activity are more soluble in organic solvents. These studies provide an evidence to support traditional medicinal uses of the plant.

PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL ACTIVITY OF MORINGA OLEIFERA

TJPRC, 2014

The aim of the present study was to find out antibacterial property of Moringa oleifera, family Moringaceae. Moringa oleifera is a very useful tree in tropical countries. In ayurvedic all parts of the tree used in different healing procedures for different diseases. The plant leaves are very good nutrient supplement for malnutrition and also used as an antibiotic. To evaluate the antibacterial activity of Moringa oleifera leaf extracts, Escherichia coli, Pseudomonas aeroginosa, Staphylococcus aureus, Proteus vulgaris, Streptococcus mutans, Bacillus subtilus, and Staphylococcus epidermidis bacteria were used. Phytochemical analysis of the leaf in solvents of varying polarity; viz., aqueous, ethanol were also carried out. The phytochemical screening indicated the presence of flavonoids, tannins, steroid, alkaloid, saponins etc., in the both extracts. Well diffusion method was used to assess the antibacterial effect of the extracts on micro-organisms. The ethanolic and aqueous extract were active against all strains but the ethanol leaf extract showed maximumactivity against Streptococcus mutantandaqueous extract shows maximum activity against Proteus vulgaris.

PHYTOCHEMICAL AND ANTIBACTERIAL OF MORINGA OLEIFERA LEAVES

World Journal of Pharmaceutical Research

Background and Objective: The aim of this study was the determine in vitro antibacterial activity of different extracts of Moringa oleifera leaves against clinical isolates and standard bacteria, determine the Minimum Inhibitory Concentrations (MICs) and identify the major chemical components of the plants extracts. Material and Method: The cup agar plate diffusion method was used to screen the antibacterial activity of plant extracts and antibiotic. The Minimum Inhibitory Concentrations (MICs) of the most active extracts were determined using the agar plate dilution method. All data were expressed as mean ± standard Deviation mean. Analysis of variance was performed by ANOVA using the SPSS software. Significant differences between means were determined by Least Significant Difference (L.S.D) and student t-test. A significant difference was considered at the level of P <0.05. Results: The results indicated that most extracts exhibited inhibitory effect against stranded bacteria and isolates. The minimum inhibitory concentrations (MICs) ranged between 25 mg/ml and 50mg/ml for all the organisms. The methanolic extract of Moringa oleifera leaves is active against the tested gram positive and gram negative microorganism. The phytochemical screening reveal the presence of flavonoids, sterols, coumarins tannins, alkaloids, saponins, triterpenes and anthraquinones.

Antimicrobial Activities and Phytochemical Investigation of Moringa Oleifera Lam. Leaf Extracts

https://www.ijrrjournal.com/IJRR\_Vol.4\_Issue.4\_April2017/Abstract\_IJRR002.html, 2017

Phytochemicals investigation and Antimicrobial activities of water and methanol extracts of Moringa Oleifera leaf were investigated in an attempt to evaluate its antimicrobial potentials. The inhibition was determined using Ditch method against four selected bacterial species and three fungal species. The phytochemical screening indicated the presence of alkaloids, flavonoids, glycoside, saponin, steroids, tannins and volatile oil. Both water and methanol extracts showed highest antifungal activity against S. cerevisiae with zones of inhibition of 9, 12 and 14 mm at the concentration of 60, 90 and 120 mg/ml respectively in water extract; and inhibition zones of 11, 15, 16 and 18 mm at the concentration of 30, 60, 90 and 120 mg/ml respectively in methanol leaves extract of M. oleifera. A flavus on the other hand was the resistant fungal species to the M. Oleifera extracts showing no visible zone of inhibition in water extract; and exhibiting inhibition in methanol with 7 and 9.5 mm zones of inhibition at 90 and 120 mg/ml respectively. The antifungal standard drug ketoconazole exhibited highest activity against C. albicans with 19 mm zone of inhibition, followed by S. cerevisiae 15 mm zone of inhibition. In methanol extracts against Pseudomonas aeruginosa shows the highest inhibition of 15.00 mm at the highest concentration (120mg/ml), while Escherichia coli and Micrococcus species had 14.00 mm and Staphylococcus aureus with least inhibition of 8.33mm at the lowest concentration (30mg/ml). The zones of inhibition of tetracycline on test bacteria showed that P. aeruginosa was the most susceptible with the diameter of 21 mm, followed by S. aureus with 20 mm. The results obtained provide a support for the utilization of this plant in traditional medicine and recommend its further investigation of it phytochemicals.

Phytochemical screening of Moringa oleifera leaf extracts and their antimicrobial activities

Cellular and Molecular Biology, 2020

Moringa oleifera is a tree native to tropical and subtropical regions of South India and used in traditional medicine. The aim of this study was characterize the phytochemicals present in M. oleifera leaf extracts and study their antimicrobial activities. Solvent extractions with Soxhlet apparatus of leaves were obtained using hexane, benzene, isopropanol, methanol, and water. The crude extracts were concentrated and screened for qualitative phytochemical analysis, and the antibacterial, antifungal and antiviral activities of crude extracts were measured by in vitro methods. Alkaloids, carbohydrates, tannins, phenolic compounds, terpenoids, cardiac glycosides, amino acids, oils and fats were found in the different crude extracts analyzed. Water and methanol extracts showed antibacterial activity against all selected bacteria, hexane and benzene extracts showed antifungal activity against all fungi tested, and hexane, benzene and isopropanol extracts showed activity against Hepatitis...

Antimicrobial activities of Moringa oleifera Lam leaf extracts

AFRICAN JOURNAL OF BIOTECHNOLOGY, 2012

Plants have been reported to contain important preventative and curative compounds. Studies were conducted to determine the antimicrobial activities of Moringa oleifera extracts using in vitro antimicrobial screening methods. The acetone extract of M. oleifera leaves at a concentration of 5 mg/ml showed antibacterial activities against Escherichia coli, Enterobacter cloace, Proteus vulgaris, Staphylococcus aureus and Micrococcus kristinae. M. kristinae was the most susceptible as its growth was inhibited at 0.5 mg/ml. On the other hand, M. oleifera acetone extract did not exhibit any inhibition on Streptococcus faecalis, Bacillus pumilus, Klebsiela pneumonia, Bacillus cereus and Pseudomonas aeruginosa. The acetone extract was bactericidal on E. coli and M. kristinae. It was also bacteriostatic on S. aureus, E. cloace and P. vulgaris. However, the water extract showed no activity at the highest concentration (5 mg/ml) tested. Furthermore, both the acetone and aqueous extracts did not exhibit any antifungal activity against the fungal species of Candida albicans, Pennicillium notatum, Aspergillus flavus and Aspergillus niger even at the highest concentration of 10 mg/ml. The ability of acetone extract to inhibit the growth of some strains of bacteria is an indication of its antibacterial potential which may be employed in the management of microbial infections.

Phytochemical And Antimicrobial Studies On Moringa Oleifera Leaves Extracts

IOSR Journal of Environmental Science, Toxicology and Food Technology, 2014

The antimicrobial and medicinal properties of Moringa leaf extracts was examined through Phytochemical screening on the solvent extract and conducting bioassay on the target microorganisms: E. coli and Salmonella. The phytochemical screening of the plants constituents were assessed by using qualitative methods. Tests were conducted for the presence of the following active components: alkaloid, tannins, phlobatannins, phenol, flavonoids, glycoside, saponins, volatile oil, hydrolysable tannin and protein. All were present. The ethanol extract of the leaf was active against E. coli and salmonella measuring clear zones of inhibition 3.0mm and 14.0mm respectively. This indicate the presence of these bioactive components in Moringa, which proves its potency in the treatment of some bacterial infections

’Anti-Bacterial/Anti-Fungal Activity of Ethanolic and Aqueous Extracts of Moringa Oleifera Leaves

Moringa oleifera, an important medicinal plant is one of the most widely cultivated species of the family Moringaceae. It is highly valued from time immemorial because of its vast medicinal properties. The antibacterial and antifungal activity of leaves of Moringa oleifera was studied in this research work. Cold extraction method was carried out on grinded M. oleifera leaves to prepare the crude ethanolic and aqueous extracts. Five strains of Bacteria: Escherichia coli, Klebsiella, Staphylococcus aureus, Proteus, Pseudomonas aeruginosa and a fungal strain, Aspergillus niger were used for the experiment and maintained on suitable culture media at 37oc.

Biochemical Studies on Moringa Oleifera Leaves Extract

Phytochemical screening were determined in Moringa oleifera leaves. The total polyphenols and flavonoids contents of Moringa oleifera leaves have the highest of total polyphenols and flavonoids contents, which were 129.44mgGAE/g and 20.43mgGAE/g, respectively. Methanolic and aqueous extracts of plant leaves was antioxidant activity by used (FRAP, LPO, OH, DPPH and ABTS) The methanolic extract of Moringa oleifera leaves have the highest of reducing power which was ranged from 0.818 to 3.021 at the concentrations of 20 and 80 mg/ml, respectively. Also, by used (LPO, OH, DPPH and ABTS), were the highest antioxidants activity for methanolic extract respectively. Moreover, The methanolic extract of Moringa oleifera leaves produced the highest growth inhibition (20 and 17mm) for against Escherichia coli and Bacillus subtillis at 4mg/ml, respectively. While, the aqueous extracts highest growth inhibition (13mm) of against St.coccus aureus at 4mg/ml.