Effects of Bene (Pistacia atlantica) on Histopathology of Testis, Sperm Chromatin Quality and Stress Oxidative in Busulfan-Induced Infertile Mice (original) (raw)
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Systems Biology in Reproductive Medicine
Here, we studied the protective effects of Satureja Khuzestanica essential oil (SKEO) as a potent anti-oxidant, against damage caused by chemotherapy with busulfan in testis and epididymal sperm of adult mice. The NMRI adult mice were assigned: G1: control, G2: was treated with busulfan (4 days, 3.2 mg/kg), G3: was treated with busulfan (4 days, 3.2 mg/kg) and SKEO (28 days, 225 mg/kg) at the same time, and G4: was pre-treated with SKEO (7 days, 225 mg/kg) and subsequently co-treated with busulfan (4 days, 3.2 mg/kg) and SKEO (28 days, 225 mg/kg). Apoptosis and Bcl-2 family gene expression were evaluated in sperm by the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) assay and Real-Time PCR, respectively. The level of oxidative stress was studied in sperm and testis by Superoxide Dismutase (SOD) and Glutathione Peroxidase (GPx) assays. Lactate Dehydrogenase (LDH), and Thiobarbituric assays were used for analyzing cytotoxicity and lipid peroxidation in testis and sperm of mice, (TBA) respectively. The results showed a significant decrease in the percentage of apoptotic sperm in G4 versus G2 and G3 (p < 0.05). SKEO pre-treatment potentially increased Bcl-2 expression and decreased BAX expression in sperm of G4 compared with G2 and G3. The activities of SOD and GPx were increased, also, LDH and TBA decreased significantly in testis and sperm of G4 compared with G2 and G3 (p < 0.05). SKEO pre-treatment had a notable role in reducing oxidative stress, apoptosis, cytotoxicity, and genotoxicity in sperm of busulfan-treated mice. In addition, cytotoxicity and oxidative stress were decreased significantly in testes of this group. Thereby, SKEO may inhibit busulfan-mediated apoptosis in sperm via decreasing oxidative stress and regulating Bcl-2 family genes expression. In conclusion, the beneficial properties of SKEO pretreatment and co-treatment by its herbal potent anti-oxidants may reduce adverse effects of chemotherapy in the reproductive system in a rodent system.
2009
It has been established that antioxidants such as Vitamins C and E are active enhancers of gonadal functions 1-3 . This is due to the fact that they scavenge free radicals (oxidants) in tissues and blood vessels neutralizing their damaging effects. Antioxidants increase gonadal functions by enhancing the hormonal secretions necessary for the proliferation of spermatogenic cells 3 Beniseed oil has been implicated to regulate blood pressure to normal in adult hypertensive patients. Upon withdrawal, the blood pressures were elevated 4 . This might be due to the fact that it contains antioxidants such as sesamin (0.34 to 1.13%), sesamolin (0.13 to 0.58%) and sesamol. Sudesh & Vicki 5 reasoned that antioxidants may achieve antihypertensive effects through one of three ways: reducing aldehyde conjugate/AGE formation and oxidative stress, normalizing calcium channels and peripheral vascular resistance, or by improving insulin resistance and endothelial function. In the presence of oxidants, constriction of the blood vessels and subsequent hypertension may result. In the same study, antioxidants (Vitamin C & E, b-carotene and reduced glutathione) were increased. These types of effects are pro-fertility in nature. We earlier argued that Beniseed enhances fertility by production of substances necessary for the proper function of 6 . Chang et al 7 stated that schisandrin B-sesamin mixture administered to male Sprague-Dawley rats exerted a hepatoprotective effects in CC4-induced oxidative stress. This plant seed was able to do this due to its antioxidant potency as earlier evaluated by Oluyemi et al 8 Among all reactive oxygen species, hydrogen peroxide is the most toxic to testicular functions. 9 . Treatment with superoxide dismutase and catalase reduced the degree of testicular damage in experimental acute torsion by scavenging the superoxide radicals and converting them into hydrogen superoxide and water and the catalase then converts the hydrogen peroxide produced into water and oxygen 10 . These reactive oxygen species are regularly formed during the process of normal respiration. However, the production is kept at physiologically low levels by intracellular free radical scavengers 11 .
Andrologia, 2020
Although semen cryopreservation has unfavourable effects on mammalian spermatozoa (Yeste, 2016), using antioxidants prior to these freezing procedures has significant advantages (Büyükleblebici, Büyükleblebici, Taşdemir, & Tuncer, 2016). Prior to chilling and freezing spermatozoa, treatment at room temperature may produce toxic oxidants and reactive oxygen species (ROS) in the extender (Aitken, Irvine, & Wu, 1991). ROS are unstable, chemically reactive species that contain hydrogen peroxide and superoxide radicals. They attack and damage biological molecules, such as lipids and proteins, resulting in oxidative stress (Shin, Yoo, Min, Lee, & Choim, 2010). Therefore, ROS may have negative effects by breaking the membrane of the sperm cell, thus damaging the energy sources necessary for its survival (Guthrie & Welch, 2012), while oxidative stress may also result in chromatin damage (Taşdemir et al., 2013). The effects of several plant extracts on fertility have been demonstrated in many animal species (Sharma, Goyal, & Bhat, 2013). However, it is known that the antioxidant property of these extracts is correlated with their free radical scavenging properties (Laura et al., 2011). The results of a recent study suggested that plant extracts may play an important role in enhancing endogenous antioxidant defence capacity (Zhang, Luo, Chang, Jiao, & Liu, 2017). Most herbs and herb-derived products offer a large variety of one or more active compounds (D'Cruz, Vaithinathan, Jubendradass, & Mathur, 2010). Recently, evaluation of plants to identify efficient and safe
Anatomical Sciences Journal, 2020
Introduction: Spermatogenesis is a process in which sperm is produced, and its disruption at any stage can lead to infertility. Plant extracts have strong phytochemicals, like s anthocyanin. Applying decoction of this plant’s leaves could relieve nausea, and its roots are used to treat dysentery. Methods: The Naval Medical Research Institute (NMRI) mice were used and grouped into two control and treatment groups. The control group received distilled water and, the treatment group was fed with 250 mg/kg.bw mixture of plants daily after the disruption with Carbon Tetrachloride (CCl4) for 60 days. After this period, the mice got unconscious, and their testicles were removed from the abdomen. After conducting the morphologic study, including measuring the samples’ dimensions and weight, their testicles were transcended and stained with eosin hematoxylin method. All data were analyzed by SPSS V. 22. The significance level was set at P<0.05. Results: The study results revealed significant differences between the testicular size and weight. Moreover, the number of spermatogonia, spermatocytes, spermatozoa, and Leydig cells increased in the experimental group, compared to the controls (P<0.01). Conclusion: The mixture of the plant caused a significant increase in spermatogenesis cells in male mice and increased their fertility.
Male Infertility: The Effect of Natural Antioxidants and Phytocompounds on Seminal Oxidative Stress
Diseases, 2017
Defective sperm function has been identified as the most common cause of infertility. The objective of this study was to review recent findings on the effects of various antioxidants on male fertility. High amounts of poly unsaturated fatty acid are found in the mammalian spermatozoa membranes, thereby making them susceptible to lipid peroxidation. Although, free radicals and reactive oxygen species (ROS) play major roles in reproduction, they are strongly associated with oxidative stress. Furthermore, factors such as obesity, inflammation, pollutants and cigarette smoking are negatively correlated with spermatogenesis. Endogenous antioxidants system exists to mediate these damages. In a normal physiological state, the seminal plasma contains antioxidant enzyme mechanism that is capable of quenching these ROS as well as protecting the spermatozoa against any likely damage. However, high level of ROS triggered by inflammatory cells and oxidation of fatty acid in obese subjects may down play antioxidant mechanism resulting in oxidative stress. Evaluation of such oxidative stress is the first step in the treatment of male infertility through administration of suitable antioxidant. Notably, antioxidant such as vitamin E and C, carotenoids and carnitine have been found beneficial in restoring a balance between ROS generation and scavenging activities. There are emerging evidences that herbal products can also boost male reproductive functions. Nonetheless, a good lifestyle, regular exercise, avoidance of stress and observing safety rules at work are habits that can reverse male infertility.
International Journal of Advance Research, Ideas and Innovations in Technology, 2018
One of the important concerns today is the problem of Overpopulation. Population explosion is creating worldwide problems. The population of the world is growing faster than the supplies of food, shelter and fuel. The increase in population is becoming a comprehensive dilemma, causing much pressure on economic, social and natural assets. In the present scenario world's population has amplified at an alarming rate and is the main cause of poverty. World Health Organization (WHO) noted that majority of the World's population depends on traditional medicine for primary healthcare. Population control is a significant issue worldwide especially in developing countries like India. Modern reproductive biomedicine has provided several preventive and effective methods of contraceptives for fertility control in male and female but none of which is very safe and without any serious side-effects. This overpopulation can be checked through biological means with special reference to modulation in the human fertility ability. Along with the advancement in the reproductive biomedicine difference hormonal contraceptive pills are developing but all have side effects. There is still a worldwide unmet need for more affordable, effective and practical contraceptives, indicating that further technological advancement or innovations to existing products are required. The oral contraceptive pill for women has had the significant impact on societal dynamics and socioeconomic benefits, while the development of male contraceptive options equivalent to female products has proven an elusive goal. The main reason for this is that while sperm production can be controlled by the administration of sex steroids, there is also a decrease in testosterone that requires "add-back" therapy. The World Health Organization suggested that practice of use of traditional medicine for the control of fertility, instead of synthetic drugs, as cost-effective management for birth control. For this World Health Organization has given great emphasis on folklore use of the anti-fertility herbs. In the recent years number of plants have been identified and evaluated for their anti-fertility activity. So, formulation of new herbal medicines has become a growing trend in modern ongoing experiments which includes the use of different plant parts extracts having anti-spermatogenic activities but their exact mechanism of action is not cleared. Initiative has been taken globally to find out the efficiency of herbal products for male contraceptive.
Nutrition and Cancer, 2016
The aim of this study was to investigate whether cinnamon bark oil (CBO) has protective effect on taxanes-induced adverse changes in sperm quality, testicular and epididymal oxidant/antioxidant balance, testicular apoptosis, and sperm DNA integrity. For this purpose, 88 adult male rats were equally divided into 8 groups: control, CBO, docetaxel (DTX), paclitaxel (PTX), DTXCPTX, DTXCCBO, PTXCCBO, and DTXCPTXCCBO. CBO was given by gavage daily for 10 weeks at the dose of 100 mg/kg. DTX and PTX were administered by intraperitoneal injection at the doses of 5 and 4 mg/ kg/week, respectively, for 10 weeks. DTXCPTX and DTXCPTXCCBO groups were treated with DTX during first 5 weeks and PTX during next 5 weeks. DTX, PTX, and their mixed administrations caused significant decreases in absolute and relative weights of all reproductive organs, testosterone level, sperm motility, concentration, glutathione level, and catalase activity in testicular and epididymal tissues. They also significantly increased abnormal sperm rate, testicular and epididymal malondialdehyde level, apoptotic germ cell number, and sperm DNA fragmentation and significantly damaged the histological structure of testes. CBO consumption by DTX-, PTX-, and DTXCPTX-treated rats provided significant ameliorations in decreased relative weights of reproductive organs, decreased testosterone, decreased sperm quality, imbalanced oxidant/ antioxidant system, increased apoptotic germ cell number, rate of sperm with fragmented DNA, and severity of testicular histopathological lesions induced by taxanes. In conclusion, taxanes cause impairments in sperm quality, testicular and epididymal oxidant/antioxidant balance, testicular histopathological structure, and sperm DNA integrity, and long-term CBO consumption protects male reproductive system of rats.
Malaysian Applied Biology, 2017
This study was carried out to compare the effects of ethanolic extract of Cosmos caudatus (CCEE), Piper sarmentosum (PSEE) and Premna cordifolia (PCEE) on sperm parameters of male mice, Mus musculus. Twenty four sexually matured male mice were used in this study. The mice were grouped into 4 groups of 6 mice each. The first group was given 0.9% saline solution and served as a control whereas groups 2-4 were daily administered with 500 mg/kg body weight of CCEE, PSEE and PCEE respectively, orally for 30 days daily. The body weight was recorded prior to, and after the treatments. At the end of the experimental period, the mice were sacrificed by cervical dislocation. The testis and epidydimis were collected and weighed. Then, the sperm suspension was prepared and subjected to sperm analysis (sperm motility, count and morphology). Bodyweight and reproductive organ weight (testis and epididymis) were not significantly different between control and mice treated with CCEE, PSEE and PCEE. Sperm count was increased in all groups compared to control but not significantly different. Sperm motility of mice treated with PSEE significantly (p<0.05) increased compared to control. Meanwhile sperm motility was not affected by CCEE and PCEE. PCEE significantly (p<0.05) decreased the percentage of normal sperm morphology compared to control. CCEE and PSEE also reduced the percentage of normal sperm morphology but the values were not significantly different. This study proves that all of the three plants affect the sperm quality, thus further study is suggested.
Urology Journal, 2021
Oilseeds and their related products are known to have various bioactive and healthpromoting ingredients. In this research, we investigated the effects of phytosterols and fatty acids of Pistacia vera on spermatogenesis process and testis histological changes in Wistar male rats for the first time. Materials and Methods: A total number of 64 adult male Wistar rats were divided randomly into eight groups including one control group, and seven test groups. Test groups received phytosterols, fatty acids, and pistachio oil orally for 30 days. Then, LH, FSH and serum testosterone levels were determined. Also, the spermatogenesis process and changes in testicular tissue in rats were investigated. Results: The results of this research suggest that phytosterols in doses 10 and 50 mg/kg reduce spermatogenesis process. Fatty acid in a low dose of 10 mg/kg increases spermatogenesis, but when a high dose of 50 mg/kg was used, it harmed the spermatogenesis process. When low levels of phytosterols and fatty acids are used simultaneously in dose 5 mg/kg, improvement in spermatogenesis process is observed but when these were used together in the dose of 25 mg/kg, the spermatogenesis process was disrupted. Using pistachio oil alone also improved spermatogenesis process. Conclusion: It seems that phytosterols reduce spermatogenesis at high and low doses, while fatty acids increase spermatogenesis when used in low doses and reduce this process when used in high doses. The use of fatty acids extracted from pistachios to treat infertility in men seems hopeful
International Journal of Medical and Biomedical Studies, 2020
Background: Several Ayurvedic textbooks describe Sida cordifolia Linn. and Glycyrrhiza glabra Linn. for their anti-inflammatory, antipyretic, antioxidant and sexual properties. After obtaining good results in treatment of male sexual disorders during pre-clinical studies, this clinical trial was taken up to assess the antioxidant properties responsible for spermatogenetic activity of aqueous extract of roots of these two plants. Methods: The study uses subjective evaluation of primary symptoms, estimation of Testosterone levels, sperm analysis and evaluation of oxidative stress levels for assessing the therapeutic efficacy of research formulation through placebo controlled clinical trials on 80 males having lack of sexual desire and non-satisfactory sexual life divided in four study groups. Group I was the control group while Group II received Sida cordifolia Linn., Group III Glycyrrhiza glabra Linn. and Group IV was administered both these plants mixed equally. The tests for asses...