A rapid TLC autographic method for the detection of glucosidase inhibitors (original) (raw)

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A rapid TLC autographic method for the detection of glucosidase inhibitors

Phytochemical Analysis, 2007

Abstract

A new bioautographic assay suitable for the localisation of beta-glucosidase inhibitors present in a complex matrix is described. Enzyme activity was detected using esculin as the substrate to produce esculetin, which reacts with ferric ion to form a brown complex.

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