Long-Term Stability of Air-Dried Enzyme Electrodes with Selective Enzymic Collagen Membranes (original) (raw)

Enzyme Engineering, 1982

Abstract

Enzyme collagen membranes have been prepared with the previously described coupling procedure developed in this laboratory (1, 2). Surface binding is obtained on both faces, allowing for asymmetric coupling with bienzyme systems, each enzyme being bound on only one face of the membrane (3). In our electrodes these membranes are associated with a platinum anode for the amperometric detection of H2O2, leading to very reliable enzyme electrodes for the detection of various species with selected mono or multienzyme systems that lead to hydrogen peroxide (4, 5). At present, the storage conditions of the enzyme electrodes need to be simplified for more practical use. Previously, the sensors were kept in buffer at 4°C, and later at room temperature. Under these conditions the sensors were still usable after more than two years, provided a calibration was made for each set of measurements.

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