Conformation of human IgG subclasses in solution. Small-angle X-ray scattering and hydrodynamic studies (original) (raw)

1985, European Journal of Biochemistry

The structure of six human myeloma proteins: IgGl(Bal), IgG2(Klu), IgG3(Bak), IgG3(Het), IgG4(Kov) and IgG4(Pol), was studied in solution using small-angle X-ray scattering and hydrodynamic methods. For IgGl (Bal) and IgG3(Het) the experimental data, including radius of gyration (R",), radii of gyration of the cross-section (Rql, RqJ, intrinsic viscosity [q], sedimentation coefficient SO^^,^) and molecular mass, were interpreted in terms of structural models based on the Fab and Fc conformations, observed in crystal, by varying the relative positions of the Fab and Fc parts, i.e. their relative angles and distances.