Methodological adjustment of the accelerated aging test to evaluate the vigor of chia seeds (original) (raw)
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Methodology for germination test accomplishment in chia seeds
Agrarian
As sementes de chia são conhecidas por seus elevados teores nutricionais e por ser uma excelente fonte de energia. A chia ganhou notoriedade a partir da década de 90 com a crescente demanda por alimentos funcionais e saudáveis. Com o aumento expressivo da demanda por este produto, a produção de sementes de alta qualidade é imprescindível para alcançar elevados índices de produtividade. Além disso, existem poucos estudos sobre metodologias para análise de sementes dessa espécie, tornando este estudo relevante. Dessa forma, objetivou-se determinar a metodologia quanto à temperatura, substrato e fotoperíodo para o teste de germinação em sementes de chia. O experimento foi instalado em delineamento inteiramente casualizado, com tratamentos em esquema fatorial triplo 7 x 3 x 2 (temperaturas x substratos x fotoperíodo), com quatro repetições. Foram realizados os testes de massa de 1000 sementes, teor de água e teste de germinação com variações de temperaturas de 10, 15, 20, 25, 30, 35 e 4...
Matriconditioning effect on the physiological performance of chia seeds (Salvia hispanica L.)
Revista de Ciencias Agrícolas, 2022
Solid matrix priming (matriconditioning) permits slow seed hydration, and germination and emergence period reduction. In general, seeds need to be soaked to start their germination process. The objective of this study was to evaluate the effect of the matriconditioning under the imbibition curve and the physiological performance of two lots of chia seeds with different qualities. The study was performed with chia seeds harvested by small-scale farmers. To estimate the imbibition curve, seeds from both lots were started in the imbibition process on a stainless-steel screen placed in plastic boxes, containing 55.5 grams of sterilized vermiculite and moisturized with distilled water at 100% of their retention capability. The imbibition curve weights were made 0, 2, 4, 8, 16, and 32 hours after the process had started. Simultaneously, in each evaluation period, seeds were submitted to the following tests: germination, first count of germination, plantlet shoot, root and total lengths, emergence velocity index and average time, and accumulated emergence. A completely randomized experimental design was used. It was possible to conclude that the beginning of phase II of germination in matriconditioned seeds varies in function of the chia seeds' lot quality. The matriconditioning contributes to the physiological performance, increasing and accelerating plantlets' emergence.
Brazilian Journal of Biology Germination of chia seeds submitted to saline stress
Germination of chia seeds submitted to saline stress, 2019
Salinity, of both soil and water, is one of the main causes of crop yield decline. Within this context, the objective of this study was to evaluate the influence of different salts on the germination of chia seeds. The experiment was conducted in a BOD chamber at a constant temperature of 20 °C and in the presence of light. The seeds were placed on paper soaked with aqueous solutions of calcium chloride (CaCl 2), sodium chloride (NaCl), potassium chloride (KCl), and magnesium chloride (MgCl 2), at the osmotic potentials zero,-0.10,-0.20,-0.30, and-0.40 MPa. The effect of the salinity was evaluated using a germination test, with counts on days 7 and 14 after sowing. Based on the results, chia seeds tolerate concentrations of NaCl to-0.4 MPa and KCl to-0.20 MPa. The salts CaCl 2 and MgCl 2 had a negative effect on the germination and vigor of the chia seeds for the osmotic potentials-0.30 MPa and-0.20 MPa, respectively. Germinação de sementes de chia submetidas ao estresse salino Resumo A salinidade, tanto dos solos como das águas, é uma das principais causas da queda de rendimento das culturas. Neste contexto, o objetivo deste estudo foi avaliar a influência de diferentes sais na germinação de sementes de chia. O experimento foi conduzido em câmara BOD, na temperatura constante de 20 °C e em presença de luz. As sementes foram colocadas sobre papel embebido em soluções aquosas de cloreto de cálcio (CaCl 2), cloreto de sódio (NaCl), cloreto de potássio (KCl) e cloreto de magnésio (MgCl 2) nos potenciais osmóticos correspondentes a zero;-0,10;-0,20;-0,30 e-0,40 MPa. O efeito da salinidade foi avaliado através do teste de germinação com contagens aos 7 dias e 14 dias após a semeadura. De acordo com os resultados é possível concluir que as sementes de chia toleram concentrações de NaCl até-0,4 MPa e KCl até-0,20 MPa. Os sais CaCl 2 e MgCl 2 apresentam efeito negativo sobre a germinação e o vigor das sementes de chia a partir dos potenciais osmóticos de-0,30 MPa e-0,20 MPa, respectivamente. Palavras-chave: Salvia hispanica, salinidade, água.
Accelerated aging test and its relationship to physiological potential of soybean seeds
Journal of Seed Science, 2019
Although accelerated aging is one of the tests most used for evaluation of the physiological quality of soybean seeds, there are few studies that relate it to other physiological tests used for the crop. In this context, the objective of this study was to investigate the relationship of accelerated aging to the results of physiological performance obtained in the vigor tests most frequently used in the soybean quality control system (germination, first count, seedling vigor classification, accelerated aging, electrical conductivity, emergence speed index, final emergence in sand substrate, and field emergence). The experiment was conducted with seeds that had different vigor levels, adopting a completely randomized experimental design with four replications. Pearson simple correlation analysis (r) and simple linear regression analysis at both 1% and 5% probability were conducted on the data. The results showed that the seedling vigor classification test (r = 0.9160) and the field emergence test (r = 0.9198) were those that best correlated with accelerated aging, followed by the germination (r = 0.8690), first count of germination (r = 0.8460), and electrical conductivity (r = 0.8912) tests.
Physiological quality of popcorn seeds assessed by the accelerated aging test
Despite the insufficient internal supply of popcorn kernel cultivars, the Brazilian production is continuously increasing. Therefore, searching for tests that offer fast results and reliable information on the physiological potential of the seeds is very important. The objective of this study was to assess the efficiency of the accelerated aging test, by using different times and exposure temperatures, for the evaluation of the physiological quality of popcorn seed lots. Popcorn seeds from three lots of the hybrid AP 8203 were used. For the initial characterization of the lots, the following tests were conducted: seed moisture content, first and final germination count, and field emergence. The accelerated aging test was performed in a 3x4x2 factorial scheme. After aging, the seeds were tested for moisture content, germination, and electrical conductivity. The experiment was carried out in a completely randomized design. The characteristic vigor was affected during aging, as evidenced by the increase in the amount of leachate detected by the electrical conductivity test. Seed lot 3 was most vigorous in the aging test conducted for 48 h at 45 °C. The accelerated aging test at 42 ºC for 48 h provides consistent information to differentiate seed lots of popcorn. Index terms: vigor, germination, temperature. Qualidade fisiológica de sementes de milho pipoca avaliadas pelo teste de envelhecimento acelerado RESUMO-A oferta nacional de cultivares de milho pipoca é pequena, contudo a produção é crescente. Assim, a busca por testes que ofereçam rapidez e informações seguras sobre o potencial fisiológico das sementes torna-se de fundamental importância. Objetivou-se avaliar a eficiência do teste de envelhecimento acelerado na avaliação da qualidade fisiológica de lotes de sementes de milho pipoca, testando-se diferentes períodos e temperaturas de exposição. Foram utilizadas sementes de milho pipoca do híbrido AP 8203 provenientes de três lotes. Para a caracterização inicial dos lotes, determinou-se o teor de água, a primeira e a contagem final do teste de germinação e a emergência em canteiro. O teste de envelhecimento acelerado foi realizado em esquema fatorial 3x4x2. Após o envelhecimento, determinou-se o teor de água, e as sementes foram submetidas aos testes de germinação e condutividade elétrica. O delineamento experimental utilizado foi o inteiramente casualizado. O vigor foi afetado durante o envelhecimento devido ao aumento da quantidade de lixiviados detectado no teste de condutividade elétrica. O lote de sementes 3 foi o mais vigoroso pelo teste de envelhecimento conduzido por 48 h na temperatura de 45 ºC. O teste de envelhecimento acelerado utilizando a temperatura de 42 ºC, durante 48 h, fornece informações consistentes que permitem diferenciar lotes de sementes de milho pipoca. Termos para indexação: vigor, germinação, temperatura.
International Journal of GEOMATE
Chia (Salvia hispanica L.), a species of seeds with high essential fatty acids and nutraceutical content has encouraged increased crop production worldwide. Keeping value-added products in consideration, four experiments were conducted with the CRD method. In the first experiment, seed preparation was done by soaking the seeds in water, followed by sowing them in media. In the first treatment, T1, seeds were sown without soaking. In T2, seeds were sown after being soaked for 6 hours at room temperature (temperature) and, in T3 for 12 hours. In T4, after being soaked at 70-80°C, the seeds were allowed to cool down at room temperature for 6 hours and in T5 for 12 hours, before sowing. According to the results, T4 treatment produced the highest germination percentage, microgreen height and fresh weight. In the second experiment, seeds were sowed in 5 different seeding medias for 7 days. The mix of coconut coir with sand and rice husk ash (1:1:1) produced the highest percentage of germination, microgreen height and fresh weight. The third experiment was conducted to study the best seed sowing rate amongst 56, 93, 130, 167 and 204 g/m 2. The results showed that the seed sowing rate of 204 g/m 2 gave the highest fresh weight. The fourth experiment was to study the effect of harvesting period, by focusing on the harvesting periods of 5, 6, 7, 8 and 9 days, after sowing. The results showed that the period of 6-9 days after sowing gave the highest fresh weight.
Adequacy of the accelerated aging test for soybean seeds
Colloquim Agrariae, 2021
Seed companies use vigor tests in their internal seed quality control procedures. Therefore, it is necessary to choose effective methods to obtain quick responses for making decisions related to the handling, disposal and marketing of seed lots. Thus, the objective of this work was to adapt a methodology for the accelerated aging test in soybean seeds. Sixty-nine lots were used and the experimental design used was completely randomized, with four replications. For the initial characterization of the lots, water content was determined, germination test and field emergence. The soybean seed lots selected based on the initial characterization, were submitted to the standard accelerated aging methodology and twenty-one adapted methodologies. After the physiological characterization of the 69 lots, 24 lots with similar germination with different levels of vigor were selected. For adapted methodologies that used only distilled water inside the gerbox box, there was an increase in the wate...
Germination of chia seeds submitted to saline stress
Brazilian Journal of Biology, 2019
Salinity, of both soil and water, is one of the main causes of crop yield decline. Within this context, the objective of this study was to evaluate the influence of different salts on the germination of chia seeds. The experiment was conducted in a BOD chamber at a constant temperature of 20 °C and in the presence of light. The seeds were placed on paper soaked with aqueous solutions of calcium chloride (CaCl2), sodium chloride (NaCl), potassium chloride (KCl), and magnesium chloride (MgCl2), at the osmotic potentials zero, -0.10, -0.20, -0.30, and -0.40 MPa. The effect of the salinity was evaluated using a germination test, with counts on days 7 and 14 after sowing. Based on the results, chia seeds tolerate concentrations of NaCl to -0.4 MPa and KCl to -0.20 MPa. The salts CaCl2 and MgCl2 had a negative effect on the germination and vigor of the chia seeds for the osmotic potentials -0.30 MPa and -0.20 MPa, respectively.
Effect of the Storage Period on the Physiological Quality of Cagaita Seeds (Eugenia dysenterica DC.)
Journal of Agriculture and Ecology Research International
Cagaita seeds quickly lose their germination power when stored in the natural environmental conditions of the Cerrado. Like most native Cerrado species, data on more favorable seed viability conservation conditions are still quite scarce. The experiment was carried out in the greenhouse of the Federal Institute of Education, Science and Technology of the Tocantins in the city of Gurupi - TO, between November 5, 2019, and March 7, 2020. Cagaita seeds (Eugenia dysenterica DC.) were used to perform it. The treatments applied to the seeds consisted of five sowing times, at intervals of three days. In general, the evaluated characteristics showed sensitivity by indicating differences between sowing times, where the highest values, root, and shoot length were obtained when the seeds were sown on 11/05/2019 (12.8 cm; 10.2 cm), respectively, and lower at sowing of 11/17/2019 (7.8 cm; 7.0 cm), respectively. The values obtained from the first emergency count allowed us to differentiate the so...