Inhibition mode of promoter recognition of SigW by RsiW (original) (raw)

2017

Abstract

<p>(a) The ribbon model showing the interaction between σ^W₄ and RsiW. (b) The model of -35 element recognition by σ^W₄, generated by the superimposition with the structure of <i>E</i>.<i>coli</i> σ^E₄/-35 element (PDB ID: <b>2H27</b>). (a, b) σ^W₄ ribbon models are drawn in the same orientation. α1’ in RsiW interacts directly with the recognition helix for the -35 element binding. The N-terminal helical bundle (α1’-α3’) of RsiW sterically restricts the DNA recognition of SigW. (c) The ribbon model showing the interaction between σ^W₂ and RsiW. (d) The model of the -10 element recognition by σ^W₂, generated by the superimposition with the structure of <i>T</i>. <i>acuaticus</i> σ2/single strand -10 element (PDB ID: <b>3UGO</b>). (c, d) The σ^W₂ surface for the recognition of the -10 element is blocked by σ^W₄ binding in the SigW/RsiW complex, rather than being blocked directly by RsiW.</p

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