Capacity of passively administered antibody to prevent establishment of Brucella abortus infection in mice (original) (raw)
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Veterinary World, 2018
Aim: The major objective of the investigation was to evaluate the hitherto uncharacterized potential of Brucella-specific antibodies to win the battle against virulent Brucella abortus infection. Materials and Methods: Brucella-specific immune serum was raised in mice. The antibody titer of serum was determined by standard tube agglutination test and indirect enzyme-linked immunosorbent assays (iELISA). Groups of mice and guinea pigs were passively immunized with serum containing specific agglutinin titers. 24 h after immunization, all animals along with unimmunized controls were challenged with B. abortus S544. Total B. abortus S544 counts in the spleen of each animal collected on the 7th day of challenge was determined to evaluate the protective index (PI) of anti-Brucella serum by statistical analysis. Results: A dose-dependent protective response to immune mice serum was observed in both experimental models though the values of PI of mice were higher than those obtained for guinea pigs. The PI values in mice passively immunized with 50 IU or 25 IU antibodies were 1.38 and 0.69, respectively. In guinea pigs, however, animals passively immunized with 50 IU or 25 IU antibodies showed PI values equivalent to 0.79 and 0.41, respectively. Conclusion: The observations support our hypothesis that the presence of antibodies inhibits the initial multiplication and eventual colonization of systemic organs by B. abortus. Therefore, a predominant antibody-mediated response induced by a vaccine is expected to protect the animal against the most severe clinical outcome of infection.
Protection of Mice against Brucella Abortus by Immunization with Polyclonal Anti-Idiotype Antibodies
Immunobiology, 1990
Polyclonal goat anti-idiotypic antibodies containing internal images which mimic Brucella abortus antigens were generated from rabbit polyclonal idiotypes specific for partially purified extract of B. abortus (PX III). The anti-idiotypic antibodies were purified using two-step immunoaffinity column chromatography. The presence of internal images was demonstrated by competitive inhibition analysis using an enzyme-linked immunosorbent assay. Several groups of BALB/c mice were vaccinated with the anti-idiotypic antibodies. The vaccinated mice showed a high serum titer of antibodies specific for B. abortus. When the vaccinated mice were challenged with a virulent B. abortus strain 2308, > 90 % reduction of bacteria in the spleen as compared to the unvaccinated control groups was seen. Immunoblotting experiments using antiserum from vaccinated mice demonstrate the ability to distinguish vaccinated mice from B. abortus infected mice. Our data indicate that the anti-idiotypic antibody containing internal images of B. abortus may be used as a vaccine and the induced antibody can be distinguished by immunoblotting from antibodies generated by natural infection with B. abortus.
Humoral Immunity in mice Mediated by Monoclonal Antibodies Against the A and M Antigens of Brucella
Journal of Medical Microbiology, 1989
All smooth strains of Brucella bear two lipopolysaccharide (LPS) antigens in a ratio that defines the classification of strains in serovars, A (A > M), M (M >A) and A.M (A = M). Anti-LPS-A monoclonal antibodies (MAb-A) were previously shown to convey protection to mice against B. abortus (A) strain 544, as shown by lower spleen counts than in controls at days 7 and 21 after challenge. Anti-LPS-M monoclonal antibodies (MAb-M) were obtained and tested for M-specificity with LPS from reference strains by ELISA, by agglutination of LPS-coated latex particles, and by inhibition of this agglutination. Antigens A and M of three strains were quantified by a homologous LPS-latex and MAb agglutination inhibition assay. Protection conferred by MAb-A and MAb-M against three strains, B. abortus 544 (A), B. abortus 292 (M) and B. melitensis H38 (M)
Journal of Medical Microbiology, 1992
The effect of monoclonal antibodies (MAbs) injected alone or in combination on brucella splenic infection in CD-1 mice was tested 7 and 21 days after a challenge with virulent Brucella abortus 544. Passive immunisation of mice with anti-25-27-kDa MAb alone, or mixed with protective anti-16.5 and anti-36-38-kDa MAbs, or with MAbs of the same specificity which were previously demonstrated to have no activity on CD-1 mice, produced a significant reduction of spleen counts of B. abortus (p < 0.01). Other combinations of MAbs did not reduce splenic infection in comparison with the untreated control group. BALB/c mice were used to test the possible interference of the immune response of CD-1 mice against MAbs that were produced in BALB/c mice. No reduction of splenic infection was shown with anti-25-27-or -36-38-kDa MAbs, whereas anti-lipopolysaccharide (LPS) MAb which was produced in CBA mice was effective. Combination of anti-protein MAbs with the anti-LPS MAb produced only the effect of the anti-LPS MAb at 7 and 21 days after challenge.
Veterinary world, 2018
The major objective of the investigation was to evaluate the hitherto uncharacterized potential of -specific antibodies to win the battle against virulent infection. -specific immune serum was raised in mice. The antibody titer of serum was determined by standard tube agglutination test and indirect enzyme-linked immunosorbent assays (iELISA). Groups of mice and guinea pigs were passively immunized with serum containing specific agglutinin titers. 24 h after immunization, all animals along with unimmunized controls were challenged with S544. Total S544 counts in the spleen of each animal collected on the 7 day of challenge was determined to evaluate the protective index (PI) of anti- serum by statistical analysis. A dose-dependent protective response to immune mice serum was observed in both experimental models though the values of PI of mice were higher than those obtained for guinea pigs. The PI values in mice passively immunized with 50 IU or 25 IU antibodies were 1.38 and 0.69, ...
Vaccine, 2015
The aim of the present study was to evaluate the use of different mouse strains (BALB/c, Swiss and CD-1 ®) and different challenge strains (Brucella abortus 544 and 2308) in the study of B. abortus vaccine (S19 and RB51) immunogenicity test in the murine model. No significant difference in B. abortus vaccine potency assay was found with the use of B. abortus 544 or B. abortus 2308 as challenge strain. Results of variance analysis showed an interaction between treatment and mouse strain; therefore these parameters could not be compared separately. When CD-1 ® groups were compared, those vaccinated showed significantly lower counts than non-vaccinated ones (P < 0.05), independently of the vaccine received (S19 or RB51). Similar results were observed on BALB/c groups. However, in Swiss mouse groups, S19 was more protective than RB51 (P <0.05), which showed protection when compared to the non-vaccinated group (P < 0.05). In summary, data from the present study showed that CD-1 ® , BALB/c and Swiss mice strains, as well as both challenge strains, B. abortus strains 544 and 2308, can be used in immunogenicity tests of S19 and RB51 vaccines.
Induction of Specific Cytotoxic Lymphocytes in Mice Vaccinated with Brucella abortus RB51
Infection and Immunity, 2001
A safe, more sensitive, nonradioactive, neutral red uptake assay was adopted to replace the traditional 51 Cr release assay for detection of Brucella-specific cytotoxic T lymphocyte (CTL) activity. Our studies indicated that Brucella abortus strain RB51 vaccination of mice induced specific CTLs against both strain RB51-and strain 2308-infected J774.A1 macrophages but not against Listeria monocytogenes-infected J774.A1 cells. The antigenspecific cytotoxic activity was exerted by T lymphocytes but not by NK cells. CD3 ؉ CD4 ؉ T cells secreted the highest level of gamma interferon (IFN-␥) and were able to exert a low but significant level of specific lysis of Brucella-infected macrophages. They also exerted a low level of nonspecific lysis of noninfected macrophages. In contrast, CD3 ؉ CD8 ؉ T cells secreted low levels of IFN-␥ but demonstrated high levels of specific lysis of Brucella-infected macrophages with no nonspecific lysis. These findings indicate that B. abortus strain RB51 vaccination of mice induces specific CTLs and suggest that CD3 ؉ CD4 ؉ and CD3 ؉ CD8 ؉ T cells play a synergistic role in the anti-Brucella activity.
Iraqi Journal of Veterinary Medicine, 2015
The aim of this study was to evaluate the cellular immune responses of salt-Extractable Brucella abortus S19 antigens with immunoadjuvant soluble βeta-glucan in BALB/C mice later challenged with B. abortus virulent strain. The 0.72mg/ml of SEBA was used according to the results obtained from experiment to determine the macrophages Nitric oxide production and delayed type hypersensitivity test. One hundred BALB/C mice were divided into four groups. G1 were injected i.p with 0.2 ml of saline, G2 were vaccinated S.C with 0.1ml (108 CFU/mouse) of B. abortus S19, G3 were vaccinated i.p. with 0.2 ml of salt-Extractable Brucella abortus S19 antigens and G4 were vaccinated i.p. with 0.2 ml of salt-Extractable Brucella abortus S19 antigens and 0.2ml βeta glucan. At 27 days after immunization the delayed type hypersensitivity test was conducted with the significant (P<0.05) an increase in the foot pad thickness of the G4 as compared to G3, G2 and G1. At day 30 of immunization all rema...