Indole Acetic Acid Producing And Phosphate Solubilizing Bacteria Native To Kenyan Soils Promote Growth of Common Bean (Phaseolus Vulgaris L.) (original) (raw)
Related papers
International Letters of Natural Sciences, 2016
The effect of inoculating single cultures of plant growth-promoting rhizobacteria (PGPR) on development of white beans seedling (Phaseolus vulgaris) was analyzed. Five PGPR were isolated from the rhizosphere of Okra plants and were assessed for abilities to solubilise phosphate and produce indole acetic acid (IAA). The phosphate solubilising index ranged from 6-10 while the concentration of IAA ranged from 17.48mg/l to 27.43mg/l. Serratia sp. produced the highest concentration of IAA (27.43mg/l) and had the highest solubilisation index (10mm). Bacillus sp. produced the least amount of IAA (17.48mg/l) while Staphylococcus sp. had the least solubilisation index (6mm). The highest percentage germination of 83.3% was observed in the seedling inoculated with Bacillus sp. The influence of microbial inoculation on root and shoot elongation was studied hydroponically for 7 days. Significant increases (P< 0.05) in root elongation were observed. The highest seedling root length (18.47cm) a...
International Letters of Natural Sciences, 2016
The effect of inoculating single cultures of plant growth-promoting rhizobacteria (PGPR) on development of white beans seedling (Phaseolus vulgaris) was analyzed. Five PGPR were isolated from the rhizosphere of Okra plants and were assessed for abilities to solubilise phosphate and produce indole acetic acid (IAA). The phosphate solubilising index ranged from 6-10 while the concentration of IAA ranged from 17.48mg/l to 27.43mg/l. Serratia sp. produced the highest concentration of IAA (27.43mg/l) and had the highest solubilisation index (10mm). Bacillus sp. produced the least amount of IAA (17.48mg/l) while Staphylococcus sp. had the least solubilisation index (6mm). The highest percentage germination of 83.3% was observed in the seedling inoculated with Bacillus sp. The influence of microbial inoculation on root and shoot elongation was studied hydroponically for 7 days. Significant increases (P< 0.05) in root elongation were observed. The highest seedling root length (18.47cm) a...
Agronomía Colombiana
The use of phosphate fertilizers is generally required for normal growth and development of crops. The cost of manufactured phosphate fertilizers has increased drastically in recent years and these are becoming out of reach for low-income farmers. The objective of this research was to select phosphate-solubilizing and phytohormone-producing bacteria to improve the growth and P uptake by the common bean (IAC Alvorada) in Red-Yellow Oxisol without the addition of phosphate fertilizer. The experimental design was completely randomized with three replicates and the following treatments: non-inoculated bean plants and plants inoculated separately with seven bacterial isolates: K24, K36, K71, T30, T79, A24, and T22. The previously treated bean plants were grown in the presence and absence of phosphate fertilization. Only the plants inoculated with the isolates K36 and T79 showed higher values for the dry weight and the quantities of P and N uptake by the shoot when cultivated in the absen...
The effect of co-inoculating bacterial cultures of plant growth-promoting rhizobacteria (PGPR) on the development of white bean seedlings (Phaseolus vulgaris) and their germination potential were analyzed. Five PGPR were isolated from the rhizosphere of Okra plants and were initially assessed for their Indole acetic acid (IAA) producing ability. The IAA concentrations ranged from 17.48 mg/l to 27.43 mg/l for Serratia sp. having the highest concentration with 27.43mg/l and Bacillus sp. the least with 17.48mg/l. The bacteria species were co-inoculated under five treatments to assess germination potential. The treatments included BS + Klebsiella sp. + Micrococcus sp., Micrococcus sp. + Serratia sp., Staphylococcus sp. + Bacillus sp., Serratia sp.+ Staphylococcus sp. and Klebsiella sp. + Bacillus sp. Four treatments peaked at 50% germination potential except Klebsiella sp. + Bacillus sp. which recorded germination potential of 33.3%. The effect of these PGPR treatments on the root and shoot elongation were studied hydroponically for 7 days. Significant increases (P<0.05) in root and shoot elongation were observed. The highest seedling root length of 18.5cm was recorded in treatment Serratia sp. + Staphylococcus sp. while the highest seedling shoot length of 13.5cm was recorded in treatment Staphylococcus sp. + Bacillus sp. the use of mixed PGPR as bio-inoculants can be a contemporary sustainable practice to facilitate nutrient supply to white bean seedling.
In the greenhouse experiment, two Bacillus and two Pseudomonas strains were examined for their ability to promote common bean growth (Phaseolus vulgaris L.) when co-inoculated with Rhizobium phaseoli. Co-inoculation with Rhizobium and Pseudomonas sp. LG or Bacillus sp. Bx improved shoot dry weight, nitrogen and phosphorus contents in bean plants, compared to inoculation with Rhizobium alone. Pseudomonas sp. LG promoted bean growth and particularly P uptake more efficiently than Bacillus sp. Bx. In vitro screening for plant growth-promoting traits showed phosphate solubilization, IAA, ammonia and siderophore production by Pseudomonas sp. LG, as opposed to Bacillus sp. Bx, which showed only ammonia production. Multiple plant growth-promoting traits of Pseudomonas sp. LG may be associated with its ability to improve common bean growth and nutrient uptake efficiently.
Archives of Agronomy and Soil Science, 2011
Available phosphorus is limiting in most cultivable soils in several parts of India, including Rajasthan. Four phosphate-solubilizing bacterial strains viz. Pseudomonas fluorescens BAM-4, Burkholderia cepacia BAM-6, B. cepacia BAM-12 and Aeromonas vaga BAM-77 were isolated from the rhizosphere of pearl millet (Pennisetum glaucum, cv. Raj 171), mung bean (Phaseolus aureus, cv. RMG 492) and sesame (Sesamum indicum, cv. RT 46). To the best of our knowledge, this is the first report on phosphate solubilization by Aeromonas vaga. Seed inoculation of mung bean with or without tricalcium phosphate (TCP) was performed to study the effect of single and dual bacterial inoculations in pot trials having sterilized sandy loam soil, and was found to enhance the growth and yield of plants. The results were on a par with chemical fertilizer, single superphosphate (SSP) and commercial biofertilizers, PSB (Bacillus polymyxa) and MC (Pseudomonas striata), used as standard reference. Addition of TCP to soil gave better results and dual inoculation was more effective than single inoculation of bacteria. Among the four strains studied, A. vaga and P. fluorescens were found to be more valuable as single inoculants in terms of plant growth, whereas in combination treatments, P. fluorescens along with B. cepacia and A. vaga performed very well both in the presence and absence of TCP.
Plants
The use of phosphate-solubilizing bacteria (PSB) can be a sustainable strategy to increase phosphorus availability and promote satisfactory crop yields. The objective of this study was to evaluate whether inoculation with PSB in common bean increases (i) growth, (ii) nutrition, (iii) yield, and (iv) grain quality, and (v) reduces the chemical phosphorus application dose to obtain maximum yields. The experiment was conducted in an Oxisol using a randomized block design in a 4 × 4 factorial scheme, with four replicates, using the cultivar IAC 2051. The first factor was four doses of P2O5 (0, 20, 40 and 60 kg ha−1), and the second factor was four doses of PSB (0, 100, 200 and 300 mL ha−1). For leaf area and leaf chlorophyll content, the association of PSB inoculation with a P2O5 dose of 40 kg ha−1 promoted the best conditions for the common bean. P2O5 application increased yield by 79 kg ha−1 for each 10 kg ha−1 added. PSB inoculation at a dose of 192 mL ha−1 promoted P export of 15.3 ...
A pot experiment was carried out in sandy soil to investigate the effect of biofertilization by phosphate dissolving bacteria (PDB) and/or symbiotic N: fixer on soil microflora, N2-ase activity, nodulation, plant total nitrogen content and plant growth features. The obtained results revealed that biofertilization by a mixture of B. megaierium var . phosphaticum and R. leguminosarum and received 30 kg N/fed significantly increased N2-ase activity and nodulation, particularly at the 70th day after sowing. Data also show that inoculation of seeds and application of 30 kg N/fed was better than inoculation of seeds without inorgan,c nitrogen fertilization. Data revealed that application of GO kg N/fed gave better significant increase in all plant parameters in uninoculated treatment than application of 30 kg N/fed. INTRODUCTION Leguminous crops are of great importance for human feeding as they contain considerable amounts of plant protein and some of the essential amino acids, and for soil due to the self sufficiency for nitrogen supply. Broad bean is considered to be one of the most necessary leguminous crops, which could be cultivated in the new reclaimed soils. The cultivation of broad bean or otl1er leguminous crops in desert soil are ex-posed to great difficulties without application of R. leguminosarum. Many investigators revealed that the genus R. leguminosarum has the ability to fix atmospheric nitrogen, as well as production of growth regulators which affect to a great extent the plant growth and production (Datta and Basu 2126 Annals Of Agric. Sc., Moshtohor, Vol 38(4) 2000 1997; El-Desouky et a/., 1997). The application of moderate levels of nitrogen enhance the activity of R. leguminosarum while the higher ones hinder the production of nodule formation and activity. The abundance of mineral phosphorus in the soil cause higher activity of R. leguminosarum for nitrogen fixation and production of growth regulators. Phosphate dissolving bacteria (PDB) are considered to be the important source for increasing the solubility of phosphorus (Abd El-Magid eta/, 1992). The mixed inoculation by R. leguminosarum and PDB was found to be affected on the activity of R. leguminosarum. The aim of the present study is to examine a method for improving broad bean productivity grown in sandy soil by using biofertilization for minimizing mineral nitrogen applications for reducing environmental pollution with chemical N-fenilizers and for producing a higher yield.
Agronomy
Given the hypothesis that co-inoculation with plant growth-promoting bacteria (PGPB) enhances the beneficial effects of Rhizobium tropici with greater mineral nutrition, optimizes biological nitrogen fixation and reduces use of fertilizers in bean plants, the objective of this research was to evaluate the synergistic effects of Rhizobium tropici associated with Azospirillum brasilense, Bacillus subtilis, Pseudomonas fluorescens and their combinations, on increasing the efficiency of NPK fertilization to obtain high winter yields of the (irrigated) common bean in the Cerrado region. The experiment was carried out in the field over two years in a Rhodic Hapludox under a no-till system in Selvíria, Brazil. The experimental design comprised complete randomized blocks with four replications in a 3 × 7 factorial scheme. The treatments consisted of three doses of NPK fertilizer (control—0 kg ha−1 (control); 50% of the recommended dose; 100% of the recommended dose in two parts) and seven d...
Nitrogen (N) fixation through legume-Rhizobium symbiosis is important for enhancing agricultural productivity and is therefore of great economic interest. Growing evidence indicates that other soil beneficial bacteria can positively affect symbiotic performance of rhizobia. Nodule endophytic plant growth promoting rhizobacteria (PGPR) were isolated from common bean nodules from Nakuru County in Kenya and characterized 16S rDNA partial gene sequencing. The effect of co-inoculation of rhizobium and PGPR, on nodulation and growth of common bean (Phaseolus vulgaris L.) was also investigated using a low phosphorous soil under greenhouse conditions. Gram-positive nodule endophytic PGPR belonging to the genus Bacillus were successfully isolated and characterized. Two PGPR strains (Paenibacillus polymyxa and Bacillus megaterium), two rhizobia strains (IITA-PAU 987 and IITA-PAU 983) and one reference rhizobia strain (CIAT 899) were used in the co-inoculation study. Two common bean varieties were inoculated with Rhizobium strains singly or in a combination with PGPR to evaluate the effect on nodulation and growth parameters. Co-inoculation of IITA-PAU 987 + B. megaterium recorded the highest nodule weight (405.2 mg) compared to IITA-PAU 987 alone (324.8 mg), while CIAT 899 + B. megaterium (401.2 mg) compared to CIAT 899 alone (337.2 mg). CIAT 899 + B. megaterium recorded a significantly higher shoot dry weight (7.23 g) compared to CIAT 899 alone (5.80 g). However, there was no significant difference between CIAT 899 + P. polymyxa and CIAT 899 alone. Combination of IITA-PAU 987 and B. megaterium led to significantly higher shoot dry weight (6.84 g) compared to IITA-PAU 987 alone (5.32 g) but no significant difference was observed when co-inoculated with P. polymyxa. IITA-PAU 983 in combination with P. polymyxa led to significantly higher shoot dry weight (7.15 g) compared to IITA-PAU 983 alone (5.14 g). Plants inoculated with IITA-PAU 987 and B. megaterium received 24.0 % of their nitrogen demand from atmosphere, which showed a 31.1% increase compared to rhizobium alone. Contrast analysis confirmed the difference between the co-inoculation of rhizobia strains and PGPR compared to single rhizobia inoculation on the root dry weight. These results show that co-inoculation of PGPR and Rhizobia has a synergistic effect on bean growth. Use of PGPR may improve effectiveness of Rhizobium biofertilizers for common bean production. Testing of PGPR under field conditions will further elucidate their effectiveness on grain yields of common bean.