ISSR and RAPD analyses of species and their relationships in the genus Cirsium (Asteraceae) in Iran (original) (raw)

The species relationships of Cirsium species were studied by using RAPD and ISSR molecular markers, as well as other data provided by cytology, genome size and morphological studies. A total of 428 RAPD bands were obtained, with 236 polymorphic bands. Several specific RAPD bands were obtained for the studied species. Similarly, out of 10 ISSR primers used, six primers produced reproducible bands. A total of 90 ISSR bands were obtained, out of which 19 bands were specific bands and one band was present in all species, except one. The NJ and parsimony trees obtained on the basis of a combined data set separated the species of different sections according to the taxonomic treatment of the genus in Flora Iranica.

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