Generation of antibody activity from immunoglobulin polypeptide chains produced in Escherichia coli (original) (raw)

Properties of a human immunoglobulin epsilon-chain fragment synthesized in Escherichia coli

Peter Cattini

Proceedings of the National Academy of Sciences, 1984

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Determination of the Relative Affinities of Antibody Fragments Expressed inEscherichia coliby Enzyme-Linked Immunosorbent Assay

Jeffry Watkins

Analytical Biochemistry, 1997

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Antibodies from Escherichia coli

Andreas Plückthun

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Expression of full-length immunoglobulins in Escherichia coli: rapid and efficient production of aglycosylated antibodies

Paul Sims

Journal of Immunological Methods, 2002

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Strategies for the expression of antibody fragments in Escherichia coli

Andreas Plückthun

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Generation of a recombinant mouse-human chimaeric monoclonal antibody directed against human carcinoembryonic antigen

Lee Gill

International Journal of Cancer, 1989

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Screening and kinetic analysis of recombinant anti-CEA antibody fragments

Sarah Buxbaum

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Cloning and Expression of the Variable Regions of Anti-EGFR Monoclonal Antibody in E. coli for Production of a Single Chain Antibody

s.farajnia farajnia

Iranian Journal of Biotechnology

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Isolation of engineered, full-length antibodies from libraries expressed in Escherichia coli

Robert Mabry

Nature Biotechnology, 2007

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Expressing antibodies in Escherichia coli

Andreas Plückthun

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Immunological cross-reactivity of antibodies to a synthetic undecapeptide analogous to the amino terminal segment of carcinoembryonic antigen, with the intact protein and with human sera

Samario Chaitchik, Joseph Haimovich

Proceedings of the National Academy of Sciences, 1976

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Cloning of the immunological repertoire in Escherichia coli for generation of monoclonal catalytic antibodies: construction of a heavy chain variable region-specific cDNA library

Michelle Alting-Mees, Jay Short

Proceedings of the National Academy of Sciences, 1989

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Biotechnological Aspects of Antibody Production in E. coli

Andreas Plückthun

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Engineering and heterologous expression of antibodies in Escherichia coli

Andreas Plückthun

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Effective production of carcinoembryonic antigen by conversion of the membrane-bound into a recombinant secretory protein by site-specific mutagenesis

abbas pakdel

Pathology & Oncology Research, 2005

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Production of Antibody Fab Fragments in Escherichia coli

Hiroshi Tachibana

Cell Engineering, 2011

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Mono- and bivalent antibody fragments produced in Escherichia coli: Engineering, folding and antigen binding

Andreas Plückthun

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Identification of factors impeding the production of a single-chain antibody fragment in Escherichia coli by comparing in vivo and in vitro expression

Martin Siemann

Applied Microbiology and Biotechnology, 2003

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Synthesis and expression in Escherichia coli of cistronic DNA encoding an antibody fragment specific for a Salmonella serotype B O-antigen

David Bundle

Gene, 1991

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Expression of functional antibody Fv and Fab fragments in Escherichia coli

Andreas Plückthun

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Expression of human immunoglobulin E epsilon chain cDNA in E. coli

Masaharu Seno

Nucleic acids research, 1983

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Specificities and binding properties of eight monoclonal antibodies against carcinoembryonic antigen

Sten Hammarström

Molecular Immunology, 1982

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A multivalent recombinant antibody fragment specific for carcinoembryonic antigen

Lourdes Roque

Biotechnology and Applied Biochemistry, 2006

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In vitro characterization of a recombinant 32 P-phosphorylated anti-(carcinoembryonic antigen) single-chain antibody

Geoffrey Pietersz

Cancer Immunology, Immunotherapy, 1998

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Molecular cloning of a proteolytic antibody light chain

Sudhir Paul

The Journal of biological chemistry, 1995

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Human antibody libraries in Escherichia coli

Stefan Dubel

Journal of Biotechnology, 1995

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Antibody engineering: Advances from the use of Escherichia coli expression systems

Andreas Plückthun

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Evolution of Escherichia coli Expression System in Producing Antibody Recombinant Fragments

Annamaria Sandomenico

International Journal of Molecular Sciences

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Escherichia coli producing recombinant antibodies

Andreas Plückthun

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Humanisation and characterisation of PR1A3, a monoclonal antibody specific for cell-bound carcinoembryonic antigen

Susan Young

Cancer Immunology, Immunotherapy, 1999

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Transient expression and purification of chimeric heavy chain antibodies

Yves Durocher

Protein Expression and Purification, 2009

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Monoclonal antibodies specific for carcinoembryonic antigen and produced by two hybrid cell lines

Ernesto D'Orio

1980

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